Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold

Background and Objectives: Cardiovascular disease is one of the leading causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources of cell-based therapies in heart regeneration. There are several approaches to differentiate MSCs into cardiac-like cells, such as genet...

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Autores principales:	Samaneh Khazaei, Masoud Soleimani, Zohreh Hojati, Seyyed hossein Ahmaditafti
Formato:	article
Lenguaje:	FA
Publicado:	Qom University of Medical Sciences 2021
Materias:	cardiovascular diseases mesenchymal stem cells mir-1 organ culture techniques cell differentiation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6d6faa7f3bb8436f83dbe2103751b6ff
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spelling	oai:doaj.org-article:6d6faa7f3bb8436f83dbe2103751b6ff2021-12-05T11:40:10ZDifferentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold1735-77992008-1375https://doaj.org/article/6d6faa7f3bb8436f83dbe2103751b6ff2021-08-01T00:00:00Zhttp://journal.muq.ac.ir/article-1-3193-en.htmlhttps://doaj.org/toc/1735-7799https://doaj.org/toc/2008-1375Background and Objectives: Cardiovascular disease is one of the leading causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources of cell-based therapies in heart regeneration. There are several approaches to differentiate MSCs into cardiac-like cells, such as genetic modification. In addition, using of 3D culture, such as hydrogels, increases the efficiency of differentiation. Methods: In the present study, lentiviruses containing microRNA 1 (miR- 1) and myocardium (Myocd) were co-transducted to mouse adipose-derived MSCs. Three days after, transduced MSCs were transferred to a hydrogel containing chitosan and collagen. After 21 days, the differentiation of encapsulated cells was evaluated. In this regard, the expression of cardiac markers such as NK2 homeobox 5 (Nkx2-5), GATA binding protein 4 (Gata4) and troponin T type 2 (Tnnt2) at the level of gene and protein were investigated. Results: The results of real-time quantitative polymerase chain reaction (qRT-PCR) and immunocytochemistry showed that co-induction of miR-1 and Myocd in MSCs followed by transfer to composite hydrogel increased the expression of cardiac markers. Conclusion: The use of 3D culture such as chitosan/collagen hydrogel improves the differentiation of MSCs and subsequently obtains more mature cells for use in cell-based regenerative medicineSamaneh KhazaeiMasoud SoleimaniZohreh HojatiSeyyed hossein AhmaditaftiQom University of Medical Sciencesarticlecardiovascular diseasesmesenchymal stem cellsmir-1organ culture techniquescell differentiationMedicine (General)R5-920FAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 15, Iss 5, Pp 368-377 (2021)
institution	DOAJ
collection	DOAJ
language	FA
topic	cardiovascular diseases mesenchymal stem cells mir-1 organ culture techniques cell differentiation Medicine (General) R5-920
spellingShingle	cardiovascular diseases mesenchymal stem cells mir-1 organ culture techniques cell differentiation Medicine (General) R5-920 Samaneh Khazaei Masoud Soleimani Zohreh Hojati Seyyed hossein Ahmaditafti Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold
description	Background and Objectives: Cardiovascular disease is one of the leading causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources of cell-based therapies in heart regeneration. There are several approaches to differentiate MSCs into cardiac-like cells, such as genetic modification. In addition, using of 3D culture, such as hydrogels, increases the efficiency of differentiation. Methods: In the present study, lentiviruses containing microRNA 1 (miR- 1) and myocardium (Myocd) were co-transducted to mouse adipose-derived MSCs. Three days after, transduced MSCs were transferred to a hydrogel containing chitosan and collagen. After 21 days, the differentiation of encapsulated cells was evaluated. In this regard, the expression of cardiac markers such as NK2 homeobox 5 (Nkx2-5), GATA binding protein 4 (Gata4) and troponin T type 2 (Tnnt2) at the level of gene and protein were investigated. Results: The results of real-time quantitative polymerase chain reaction (qRT-PCR) and immunocytochemistry showed that co-induction of miR-1 and Myocd in MSCs followed by transfer to composite hydrogel increased the expression of cardiac markers. Conclusion: The use of 3D culture such as chitosan/collagen hydrogel improves the differentiation of MSCs and subsequently obtains more mature cells for use in cell-based regenerative medicine
format	article
author	Samaneh Khazaei Masoud Soleimani Zohreh Hojati Seyyed hossein Ahmaditafti
author_facet	Samaneh Khazaei Masoud Soleimani Zohreh Hojati Seyyed hossein Ahmaditafti
author_sort	Samaneh Khazaei
title	Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold
title_short	Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold
title_full	Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold
title_fullStr	Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold
title_full_unstemmed	Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold
title_sort	differentiation of mesenchymal stem cells into cardiac-like cells by co-induction of lentiviruses containing mir-1 and myocd in chitosan collagen hydrogel scaffold
publisher	Qom University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/6d6faa7f3bb8436f83dbe2103751b6ff
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Differentiation of Mesenchymal Stem Cells Into Cardiac-like Cells by Co-induction of Lentiviruses Containing Mir-1 and Myocd in Chitosan Collagen Hydrogel Scaffold

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