A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery

Munson et al. develop a high-throughput Galectin-9 reporter based endosomal escape imaging assay to probe correlations between nanoparticle-mediated uptake, endosomal escape frequency, and mRNA translation. By screening various delivery systems, modulators and combinations of nanoparticle components...

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Autores principales:	Michael J. Munson, Gwen O’Driscoll, Andreia M. Silva, Elisa Lázaro-Ibáñez, Audrey Gallud, John T. Wilson, Anna Collén, Elin K. Esbjörner, Alan Sabirsh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6d71f1a55da4454e8e456d8390871e01
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spelling	oai:doaj.org-article:6d71f1a55da4454e8e456d8390871e012021-12-02T10:54:22ZA high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery10.1038/s42003-021-01728-82399-3642https://doaj.org/article/6d71f1a55da4454e8e456d8390871e012021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01728-8https://doaj.org/toc/2399-3642Munson et al. develop a high-throughput Galectin-9 reporter based endosomal escape imaging assay to probe correlations between nanoparticle-mediated uptake, endosomal escape frequency, and mRNA translation. By screening various delivery systems, modulators and combinations of nanoparticle components, the authors demonstrate how nanomedicines can be optimized for superior escape properties and efficacy in specific applications.Michael J. MunsonGwen O’DriscollAndreia M. SilvaElisa Lázaro-IbáñezAudrey GalludJohn T. WilsonAnna CollénElin K. EsbjörnerAlan SabirshNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Michael J. Munson Gwen O’Driscoll Andreia M. Silva Elisa Lázaro-Ibáñez Audrey Gallud John T. Wilson Anna Collén Elin K. Esbjörner Alan Sabirsh A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery
description	Munson et al. develop a high-throughput Galectin-9 reporter based endosomal escape imaging assay to probe correlations between nanoparticle-mediated uptake, endosomal escape frequency, and mRNA translation. By screening various delivery systems, modulators and combinations of nanoparticle components, the authors demonstrate how nanomedicines can be optimized for superior escape properties and efficacy in specific applications.
format	article
author	Michael J. Munson Gwen O’Driscoll Andreia M. Silva Elisa Lázaro-Ibáñez Audrey Gallud John T. Wilson Anna Collén Elin K. Esbjörner Alan Sabirsh
author_facet	Michael J. Munson Gwen O’Driscoll Andreia M. Silva Elisa Lázaro-Ibáñez Audrey Gallud John T. Wilson Anna Collén Elin K. Esbjörner Alan Sabirsh
author_sort	Michael J. Munson
title	A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery
title_short	A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery
title_full	A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery
title_fullStr	A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery
title_full_unstemmed	A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery
title_sort	high-throughput galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional rna delivery
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6d71f1a55da4454e8e456d8390871e01
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A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery

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