High-throughput automated microfluidic sample preparation for accurate microbial genomics

Shotgun DNA sequencing experiments for microbial genomic analysis are often impractical due to minimum sample input requirements. Here the authors develop a microfluidic sample preparation platform that reduces sample input requirements 100-fold and enables high throughput sequencing from low number...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Soohong Kim, Joachim De Jonghe, Anthony B. Kulesa, David Feldman, Tommi Vatanen, Roby P. Bhattacharyya, Brittany Berdy, James Gomez, Jill Nolan, Slava Epstein, Paul C. Blainey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6d75a655946e42d5bd25262a8dafd41e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6d75a655946e42d5bd25262a8dafd41e
record_format	dspace
spelling	oai:doaj.org-article:6d75a655946e42d5bd25262a8dafd41e2021-12-02T14:42:05ZHigh-throughput automated microfluidic sample preparation for accurate microbial genomics10.1038/ncomms139192041-1723https://doaj.org/article/6d75a655946e42d5bd25262a8dafd41e2017-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms13919https://doaj.org/toc/2041-1723Shotgun DNA sequencing experiments for microbial genomic analysis are often impractical due to minimum sample input requirements. Here the authors develop a microfluidic sample preparation platform that reduces sample input requirements 100-fold and enables high throughput sequencing from low numbers of cells.Soohong KimJoachim De JongheAnthony B. KulesaDavid FeldmanTommi VatanenRoby P. BhattacharyyaBrittany BerdyJames GomezJill NolanSlava EpsteinPaul C. BlaineyNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Soohong Kim Joachim De Jonghe Anthony B. Kulesa David Feldman Tommi Vatanen Roby P. Bhattacharyya Brittany Berdy James Gomez Jill Nolan Slava Epstein Paul C. Blainey High-throughput automated microfluidic sample preparation for accurate microbial genomics
description	Shotgun DNA sequencing experiments for microbial genomic analysis are often impractical due to minimum sample input requirements. Here the authors develop a microfluidic sample preparation platform that reduces sample input requirements 100-fold and enables high throughput sequencing from low numbers of cells.
format	article
author	Soohong Kim Joachim De Jonghe Anthony B. Kulesa David Feldman Tommi Vatanen Roby P. Bhattacharyya Brittany Berdy James Gomez Jill Nolan Slava Epstein Paul C. Blainey
author_facet	Soohong Kim Joachim De Jonghe Anthony B. Kulesa David Feldman Tommi Vatanen Roby P. Bhattacharyya Brittany Berdy James Gomez Jill Nolan Slava Epstein Paul C. Blainey
author_sort	Soohong Kim
title	High-throughput automated microfluidic sample preparation for accurate microbial genomics
title_short	High-throughput automated microfluidic sample preparation for accurate microbial genomics
title_full	High-throughput automated microfluidic sample preparation for accurate microbial genomics
title_fullStr	High-throughput automated microfluidic sample preparation for accurate microbial genomics
title_full_unstemmed	High-throughput automated microfluidic sample preparation for accurate microbial genomics
title_sort	high-throughput automated microfluidic sample preparation for accurate microbial genomics
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6d75a655946e42d5bd25262a8dafd41e
work_keys_str_mv	AT soohongkim highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT joachimdejonghe highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT anthonybkulesa highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT davidfeldman highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT tommivatanen highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT robypbhattacharyya highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT brittanyberdy highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT jamesgomez highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT jillnolan highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT slavaepstein highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics AT paulcblainey highthroughputautomatedmicrofluidicsamplepreparationforaccuratemicrobialgenomics
_version_	1718389813361508352

High-throughput automated microfluidic sample preparation for accurate microbial genomics

Ejemplares similares