QTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing

Abstract Mechanisms that regulate silk protein synthesis provide the basis for silkworm variety breeding and silk gland bioreactor optimization. Here, using the pooling sequencing-based methodology, we deciphered the genetic basis for the varied silk production in different silkworm strains. We iden...

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Autores principales:	Chunlin Li, Xiaoling Tong, Weidong Zuo, Yue Luan, Rui Gao, Minjin Han, Gao Xiong, Tingting Gai, Hai Hu, Fangyin Dai, Cheng Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d78b6ff97744f10b7c15d3652a99421
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spelling	oai:doaj.org-article:6d78b6ff97744f10b7c15d3652a994212021-12-02T15:05:20ZQTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing10.1038/s41598-017-18277-y2045-2322https://doaj.org/article/6d78b6ff97744f10b7c15d3652a994212017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-18277-yhttps://doaj.org/toc/2045-2322Abstract Mechanisms that regulate silk protein synthesis provide the basis for silkworm variety breeding and silk gland bioreactor optimization. Here, using the pooling sequencing-based methodology, we deciphered the genetic basis for the varied silk production in different silkworm strains. We identified 8 SNPs, with 6 on chromosome 11 and 1 each on chromosomes 22 and 23, that were linked with silk production. After conducting an association analysis between gene expression pattern, silk gland development and cocoon shell weight (CSW), BMGN011620 was found to be regulating silk production. BMGN011620 encodes the 60S ribosomal protein, L18, which is an indispensable component of the 60S ribosomal subunit; therefore we named it BmRPL18. Moreover, the clustering of linked SNPs on chromosome 11 and the analysis of differentially expressed genes reported in previous Omics studies indicated that the genes regulating silk protein synthesis may exhibit a clustering distribution in the silkworm genome. These results collectively advance our understanding of the regulation of silk production, including the role of ribosomal proteins and the clustered distribution of genes involved in silk protein synthesis.Chunlin LiXiaoling TongWeidong ZuoYue LuanRui GaoMinjin HanGao XiongTingting GaiHai HuFangyin DaiCheng LuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chunlin Li Xiaoling Tong Weidong Zuo Yue Luan Rui Gao Minjin Han Gao Xiong Tingting Gai Hai Hu Fangyin Dai Cheng Lu QTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing
description	Abstract Mechanisms that regulate silk protein synthesis provide the basis for silkworm variety breeding and silk gland bioreactor optimization. Here, using the pooling sequencing-based methodology, we deciphered the genetic basis for the varied silk production in different silkworm strains. We identified 8 SNPs, with 6 on chromosome 11 and 1 each on chromosomes 22 and 23, that were linked with silk production. After conducting an association analysis between gene expression pattern, silk gland development and cocoon shell weight (CSW), BMGN011620 was found to be regulating silk production. BMGN011620 encodes the 60S ribosomal protein, L18, which is an indispensable component of the 60S ribosomal subunit; therefore we named it BmRPL18. Moreover, the clustering of linked SNPs on chromosome 11 and the analysis of differentially expressed genes reported in previous Omics studies indicated that the genes regulating silk protein synthesis may exhibit a clustering distribution in the silkworm genome. These results collectively advance our understanding of the regulation of silk production, including the role of ribosomal proteins and the clustered distribution of genes involved in silk protein synthesis.
format	article
author	Chunlin Li Xiaoling Tong Weidong Zuo Yue Luan Rui Gao Minjin Han Gao Xiong Tingting Gai Hai Hu Fangyin Dai Cheng Lu
author_facet	Chunlin Li Xiaoling Tong Weidong Zuo Yue Luan Rui Gao Minjin Han Gao Xiong Tingting Gai Hai Hu Fangyin Dai Cheng Lu
author_sort	Chunlin Li
title	QTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing
title_short	QTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing
title_full	QTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing
title_fullStr	QTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing
title_full_unstemmed	QTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing
title_sort	qtl analysis of cocoon shell weight identifies bmrpl18 associated with silk protein synthesis in silkworm by pooling sequencing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6d78b6ff97744f10b7c15d3652a99421
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QTL analysis of cocoon shell weight identifies BmRPL18 associated with silk protein synthesis in silkworm by pooling sequencing

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