The healing of alveolar bone defects with novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds
Abstract Cells, scaffolds, and growth factors play important roles in bone regeneration. Bone morphogenetic protein 9 (BMP9), a member of BMP family, could facilitate osteogenesis by regulating growth factors and promoting angiogenesis. Similar to other stem cells, rat dental follicle stem cells (rD...
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2017
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oai:doaj.org-article:6d86eb6e8025464e843bf9756d3c4a1d2021-12-02T12:31:52ZThe healing of alveolar bone defects with novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds10.1038/s41598-017-06548-72045-2322https://doaj.org/article/6d86eb6e8025464e843bf9756d3c4a1d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06548-7https://doaj.org/toc/2045-2322Abstract Cells, scaffolds, and growth factors play important roles in bone regeneration. Bone morphogenetic protein 9 (BMP9), a member of BMP family, could facilitate osteogenesis by regulating growth factors and promoting angiogenesis. Similar to other stem cells, rat dental follicle stem cells (rDFCs), the precursor cells of cementoblasts, osteoblasts and periodontal ligament cells, can self-renew and exhibit multipotential capacity. Coralline hydroxyapatite (CHA) has good biocompatibility and conductivity required for bone tissue engineering. Here, we reported that BMP9 could enhance the osteogenic differentiation of rDFCs in cell culture. Moreover, our results suggested that BMP9 acted through the Smad1/5/8 signaling pathway. We also produced a novel scaffold that encompasses bio-degradable CHA seeded with recombinant adenoviruses expressing BMP9-transfected rDFCs (Ad-BMP9-transfected rDFCs). With this implant, we achieved more alveolar bone regeneration in the alveolar bone defect compared to blank group, CHA group and rDFCs group. Our results provided a novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds and its mechanism is regarding the activation of Smad1/5/8 signaling pathway in BMP9-induced rDFCs osteogenesis.Li NieXia YangLiang DuanEnyi HuangZhou PengfeiWenping LuoYan ZhangXingqi ZengYe QiuTing CaiConghua LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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Medicine R Science Q Li Nie Xia Yang Liang Duan Enyi Huang Zhou Pengfei Wenping Luo Yan Zhang Xingqi Zeng Ye Qiu Ting Cai Conghua Li The healing of alveolar bone defects with novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds |
| description |
Abstract Cells, scaffolds, and growth factors play important roles in bone regeneration. Bone morphogenetic protein 9 (BMP9), a member of BMP family, could facilitate osteogenesis by regulating growth factors and promoting angiogenesis. Similar to other stem cells, rat dental follicle stem cells (rDFCs), the precursor cells of cementoblasts, osteoblasts and periodontal ligament cells, can self-renew and exhibit multipotential capacity. Coralline hydroxyapatite (CHA) has good biocompatibility and conductivity required for bone tissue engineering. Here, we reported that BMP9 could enhance the osteogenic differentiation of rDFCs in cell culture. Moreover, our results suggested that BMP9 acted through the Smad1/5/8 signaling pathway. We also produced a novel scaffold that encompasses bio-degradable CHA seeded with recombinant adenoviruses expressing BMP9-transfected rDFCs (Ad-BMP9-transfected rDFCs). With this implant, we achieved more alveolar bone regeneration in the alveolar bone defect compared to blank group, CHA group and rDFCs group. Our results provided a novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds and its mechanism is regarding the activation of Smad1/5/8 signaling pathway in BMP9-induced rDFCs osteogenesis. |
| format |
article |
| author |
Li Nie Xia Yang Liang Duan Enyi Huang Zhou Pengfei Wenping Luo Yan Zhang Xingqi Zeng Ye Qiu Ting Cai Conghua Li |
| author_facet |
Li Nie Xia Yang Liang Duan Enyi Huang Zhou Pengfei Wenping Luo Yan Zhang Xingqi Zeng Ye Qiu Ting Cai Conghua Li |
| author_sort |
Li Nie |
| title |
The healing of alveolar bone defects with novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds |
| title_short |
The healing of alveolar bone defects with novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds |
| title_full |
The healing of alveolar bone defects with novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds |
| title_fullStr |
The healing of alveolar bone defects with novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds |
| title_full_unstemmed |
The healing of alveolar bone defects with novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds |
| title_sort |
healing of alveolar bone defects with novel bio-implants composed of ad-bmp9-transfected rdfcs and cha scaffolds |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/6d86eb6e8025464e843bf9756d3c4a1d |
| work_keys_str_mv |
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