Discovery of exolytic heparinases and their catalytic mechanism and potential application

Exolytic heparinases are needed for sequencing of heparin and heparan sulfate (HP), but have not yet been reported. Here, the authors identify exolytic heparinases from different bacteria and show that the heparinases preferentially digest HP, determine the crystal structure of the exoheparinase Ble...

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Autores principales:	Qingdong Zhang, Hai-Yan Cao, Lin Wei, Danrong Lu, Min Du, Min Yuan, Deling Shi, Xiangxue Chen, Peng Wang, Xiu-Lan Chen, Lianli Chi, Yu-Zhong Zhang, Fuchuan Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6d8a8b43feb24d34a0e24a72e67a1fa4
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spelling	oai:doaj.org-article:6d8a8b43feb24d34a0e24a72e67a1fa42021-12-02T15:52:44ZDiscovery of exolytic heparinases and their catalytic mechanism and potential application10.1038/s41467-021-21441-82041-1723https://doaj.org/article/6d8a8b43feb24d34a0e24a72e67a1fa42021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21441-8https://doaj.org/toc/2041-1723Exolytic heparinases are needed for sequencing of heparin and heparan sulfate (HP), but have not yet been reported. Here, the authors identify exolytic heparinases from different bacteria and show that the heparinases preferentially digest HP, determine the crystal structure of the exoheparinase BlexoHep and perform sequencing of HP octasaccharides using the enzyme.Qingdong ZhangHai-Yan CaoLin WeiDanrong LuMin DuMin YuanDeling ShiXiangxue ChenPeng WangXiu-Lan ChenLianli ChiYu-Zhong ZhangFuchuan LiNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Qingdong Zhang Hai-Yan Cao Lin Wei Danrong Lu Min Du Min Yuan Deling Shi Xiangxue Chen Peng Wang Xiu-Lan Chen Lianli Chi Yu-Zhong Zhang Fuchuan Li Discovery of exolytic heparinases and their catalytic mechanism and potential application
description	Exolytic heparinases are needed for sequencing of heparin and heparan sulfate (HP), but have not yet been reported. Here, the authors identify exolytic heparinases from different bacteria and show that the heparinases preferentially digest HP, determine the crystal structure of the exoheparinase BlexoHep and perform sequencing of HP octasaccharides using the enzyme.
format	article
author	Qingdong Zhang Hai-Yan Cao Lin Wei Danrong Lu Min Du Min Yuan Deling Shi Xiangxue Chen Peng Wang Xiu-Lan Chen Lianli Chi Yu-Zhong Zhang Fuchuan Li
author_facet	Qingdong Zhang Hai-Yan Cao Lin Wei Danrong Lu Min Du Min Yuan Deling Shi Xiangxue Chen Peng Wang Xiu-Lan Chen Lianli Chi Yu-Zhong Zhang Fuchuan Li
author_sort	Qingdong Zhang
title	Discovery of exolytic heparinases and their catalytic mechanism and potential application
title_short	Discovery of exolytic heparinases and their catalytic mechanism and potential application
title_full	Discovery of exolytic heparinases and their catalytic mechanism and potential application
title_fullStr	Discovery of exolytic heparinases and their catalytic mechanism and potential application
title_full_unstemmed	Discovery of exolytic heparinases and their catalytic mechanism and potential application
title_sort	discovery of exolytic heparinases and their catalytic mechanism and potential application
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6d8a8b43feb24d34a0e24a72e67a1fa4
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Discovery of exolytic heparinases and their catalytic mechanism and potential application

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