Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)

Abstract White-nose syndrome (WNS) caused by the fungus, Pseudogymnoascus destructans (Pd) has killed millions of North American hibernating bats. Currently, methods to prevent the disease are limited. We conducted two trials to assess potential WNS vaccine candidates in wild-caught Myotis lucifugus...

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Autores principales:	Tonie E. Rocke, Brock Kingstad-Bakke, Marcel Wüthrich, Ben Stading, Rachel C. Abbott, Marcos Isidoro-Ayza, Hannah E. Dobson, Lucas dos Santos Dias, Kevin Galles, Julia S. Lankton, Elizabeth A. Falendysz, Jeffrey M. Lorch, J. Scott Fites, Jaime Lopera-Madrid, J. Paul White, Bruce Klein, Jorge E. Osorio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d8f3b480a084707931474796061c861
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spelling	oai:doaj.org-article:6d8f3b480a084707931474796061c8612021-12-02T15:09:28ZVirally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)10.1038/s41598-019-43210-w2045-2322https://doaj.org/article/6d8f3b480a084707931474796061c8612019-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-43210-whttps://doaj.org/toc/2045-2322Abstract White-nose syndrome (WNS) caused by the fungus, Pseudogymnoascus destructans (Pd) has killed millions of North American hibernating bats. Currently, methods to prevent the disease are limited. We conducted two trials to assess potential WNS vaccine candidates in wild-caught Myotis lucifugus. In a pilot study, we immunized bats with one of four vaccine treatments or phosphate-buffered saline (PBS) as a control and challenged them with Pd upon transfer into hibernation chambers. Bats in one vaccine-treated group, that received raccoon poxviruses (RCN) expressing Pd calnexin (CAL) and serine protease (SP), developed WNS at a lower rate (1/10) than other treatments combined (14/23), although samples sizes were small. The results of a second similar trial provided additional support for this observation. Bats vaccinated orally or by injection with RCN-CAL and RCN-SP survived Pd challenge at a significantly higher rate (P = 0.01) than controls. Using RT-PCR and flow cytometry, combined with fluorescent in situ hybridization, we determined that expression of IFN-γ transcripts and the number of CD4 + T-helper cells transcribing this gene were elevated (P < 0.10) in stimulated lymphocytes from surviving vaccinees (n = 15) compared to controls (n = 3). We conclude that vaccination with virally-vectored Pd antigens induced antifungal immunity that could potentially protect bats against WNS.Tonie E. RockeBrock Kingstad-BakkeMarcel WüthrichBen StadingRachel C. AbbottMarcos Isidoro-AyzaHannah E. DobsonLucas dos Santos DiasKevin GallesJulia S. LanktonElizabeth A. FalendyszJeffrey M. LorchJ. Scott FitesJaime Lopera-MadridJ. Paul WhiteBruce KleinJorge E. OsorioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tonie E. Rocke Brock Kingstad-Bakke Marcel Wüthrich Ben Stading Rachel C. Abbott Marcos Isidoro-Ayza Hannah E. Dobson Lucas dos Santos Dias Kevin Galles Julia S. Lankton Elizabeth A. Falendysz Jeffrey M. Lorch J. Scott Fites Jaime Lopera-Madrid J. Paul White Bruce Klein Jorge E. Osorio Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)
description	Abstract White-nose syndrome (WNS) caused by the fungus, Pseudogymnoascus destructans (Pd) has killed millions of North American hibernating bats. Currently, methods to prevent the disease are limited. We conducted two trials to assess potential WNS vaccine candidates in wild-caught Myotis lucifugus. In a pilot study, we immunized bats with one of four vaccine treatments or phosphate-buffered saline (PBS) as a control and challenged them with Pd upon transfer into hibernation chambers. Bats in one vaccine-treated group, that received raccoon poxviruses (RCN) expressing Pd calnexin (CAL) and serine protease (SP), developed WNS at a lower rate (1/10) than other treatments combined (14/23), although samples sizes were small. The results of a second similar trial provided additional support for this observation. Bats vaccinated orally or by injection with RCN-CAL and RCN-SP survived Pd challenge at a significantly higher rate (P = 0.01) than controls. Using RT-PCR and flow cytometry, combined with fluorescent in situ hybridization, we determined that expression of IFN-γ transcripts and the number of CD4 + T-helper cells transcribing this gene were elevated (P < 0.10) in stimulated lymphocytes from surviving vaccinees (n = 15) compared to controls (n = 3). We conclude that vaccination with virally-vectored Pd antigens induced antifungal immunity that could potentially protect bats against WNS.
format	article
author	Tonie E. Rocke Brock Kingstad-Bakke Marcel Wüthrich Ben Stading Rachel C. Abbott Marcos Isidoro-Ayza Hannah E. Dobson Lucas dos Santos Dias Kevin Galles Julia S. Lankton Elizabeth A. Falendysz Jeffrey M. Lorch J. Scott Fites Jaime Lopera-Madrid J. Paul White Bruce Klein Jorge E. Osorio
author_facet	Tonie E. Rocke Brock Kingstad-Bakke Marcel Wüthrich Ben Stading Rachel C. Abbott Marcos Isidoro-Ayza Hannah E. Dobson Lucas dos Santos Dias Kevin Galles Julia S. Lankton Elizabeth A. Falendysz Jeffrey M. Lorch J. Scott Fites Jaime Lopera-Madrid J. Paul White Bruce Klein Jorge E. Osorio
author_sort	Tonie E. Rocke
title	Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)
title_short	Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)
title_full	Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)
title_fullStr	Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)
title_full_unstemmed	Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)
title_sort	virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (myotis lucifugus)
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/6d8f3b480a084707931474796061c861
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Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)

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