Systemic delivery and activation of the TRAIL gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field

Cynthia A Alvizo-Baez,1 Itza E Luna-Cruz,1 Natalia Vilches-Cisneros,2 Cristina Rodríguez-Padilla,1 Juan M Alcocer-González1 1Laboratory of Immunology and Virology, Biological Sciences Faculty, University Autonomous of Nuevo León, San Nicolás de los Garza,...

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Autores principales:	Alvizo-Baez CA, Luna-Cruz IE, Vilches-Cisneros N, Rodríguez-Padilla C, Alcocer-González JM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	magnetic nanoparticles magnetoection TRAIL chitosan apoptosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6d990cec17884724b2235cf411990690
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spelling	oai:doaj.org-article:6d990cec17884724b2235cf4119906902021-12-02T03:47:47ZSystemic delivery and activation of the TRAIL gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field1178-2013https://doaj.org/article/6d990cec17884724b2235cf4119906902016-12-01T00:00:00Zhttps://www.dovepress.com/systemic-delivery-and-activation-of-the-trail-gene-in-lungs--with-magn-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Cynthia A Alvizo-Baez,1 Itza E Luna-Cruz,1 Natalia Vilches-Cisneros,2 Cristina Rodríguez-Padilla,1 Juan M Alcocer-González1 1Laboratory of Immunology and Virology, Biological Sciences Faculty, University Autonomous of Nuevo León, San Nicolás de los Garza, 2Pahologic Anatomy and Cytopathology Service of the University Hospital, University Autonomous of Nuevo León, Monterrey, Mexico Abstract: Recently, functional therapies targeting a specific organ without affecting normal tissues have been designed. The use of magnetic force to reach this goal is studied in this work. Previously, we demonstrated that nanocarriers based on magnetic nanoparticles could be directed and retained in the lungs, with their gene expression under the control of a promoter activated by a magnetic field. Magnetic nanoparticles containing the TRAIL gene and chitosan were constructed using the ionic gelation method as a nanosystem for magnetofection and were characterized by microscopy, ζ-potential, and retention analysis. Magnetofection in the mouse melanoma cell line B16F10 in vitro induced TRAIL-protein expression and was associated with morphological changes indicative of apoptosis. Systemic administration of the nanosystem in the tail vein of mice with melanoma B16F10 at the lungs produced a very significant increase in apoptosis in tumoral cells that correlated with the number of melanoma tumor foci observed in the lungs. The high levels of apoptosis detected in the lungs were partially related to mouse survival. The data presented demonstrate that the magnetofection nanosystem described here efficiently induces apoptosis and growth inhibition of melanoma B16F10 in the lungs. This new approach for systemic delivery and activation of a gene based in a nanocomplex offers a potential application in magnetic gene delivery for cancer. Keywords: magnetic nanoparticles, magnetofection, TRAIL, chitosan, apoptosisAlvizo-Baez CALuna-Cruz IEVilches-Cisneros NRodríguez-Padilla CAlcocer-González JMDove Medical Pressarticlemagnetic nanoparticlesmagnetoectionTRAILchitosanapoptosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6449-6458 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	magnetic nanoparticles magnetoection TRAIL chitosan apoptosis Medicine (General) R5-920
spellingShingle	magnetic nanoparticles magnetoection TRAIL chitosan apoptosis Medicine (General) R5-920 Alvizo-Baez CA Luna-Cruz IE Vilches-Cisneros N Rodríguez-Padilla C Alcocer-González JM Systemic delivery and activation of the TRAIL gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field
description	Cynthia A Alvizo-Baez,1 Itza E Luna-Cruz,1 Natalia Vilches-Cisneros,2 Cristina Rodríguez-Padilla,1 Juan M Alcocer-González1 1Laboratory of Immunology and Virology, Biological Sciences Faculty, University Autonomous of Nuevo León, San Nicolás de los Garza, 2Pahologic Anatomy and Cytopathology Service of the University Hospital, University Autonomous of Nuevo León, Monterrey, Mexico Abstract: Recently, functional therapies targeting a specific organ without affecting normal tissues have been designed. The use of magnetic force to reach this goal is studied in this work. Previously, we demonstrated that nanocarriers based on magnetic nanoparticles could be directed and retained in the lungs, with their gene expression under the control of a promoter activated by a magnetic field. Magnetic nanoparticles containing the TRAIL gene and chitosan were constructed using the ionic gelation method as a nanosystem for magnetofection and were characterized by microscopy, ζ-potential, and retention analysis. Magnetofection in the mouse melanoma cell line B16F10 in vitro induced TRAIL-protein expression and was associated with morphological changes indicative of apoptosis. Systemic administration of the nanosystem in the tail vein of mice with melanoma B16F10 at the lungs produced a very significant increase in apoptosis in tumoral cells that correlated with the number of melanoma tumor foci observed in the lungs. The high levels of apoptosis detected in the lungs were partially related to mouse survival. The data presented demonstrate that the magnetofection nanosystem described here efficiently induces apoptosis and growth inhibition of melanoma B16F10 in the lungs. This new approach for systemic delivery and activation of a gene based in a nanocomplex offers a potential application in magnetic gene delivery for cancer. Keywords: magnetic nanoparticles, magnetofection, TRAIL, chitosan, apoptosis
format	article
author	Alvizo-Baez CA Luna-Cruz IE Vilches-Cisneros N Rodríguez-Padilla C Alcocer-González JM
author_facet	Alvizo-Baez CA Luna-Cruz IE Vilches-Cisneros N Rodríguez-Padilla C Alcocer-González JM
author_sort	Alvizo-Baez CA
title	Systemic delivery and activation of the TRAIL gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field
title_short	Systemic delivery and activation of the TRAIL gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field
title_full	Systemic delivery and activation of the TRAIL gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field
title_fullStr	Systemic delivery and activation of the TRAIL gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field
title_full_unstemmed	Systemic delivery and activation of the TRAIL gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field
title_sort	systemic delivery and activation of the trail gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/6d990cec17884724b2235cf411990690
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Systemic delivery and activation of the TRAIL gene in lungs, with magnetic nanoparticles of chitosan controlled by an external magnetic field

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