Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes

Abstract. Maharsiwi W, Astuti RI, Meryandini A, Wahyudi AT. 2020. Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes. Biodiversitas 21: 975-981. Exploration of new enzymes from an extreme environment is important to impr...

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Autores principales:	WENANG MAHARSIWI, RIKA INDRI ASTUTI, ANJA MERYANDINI, Aris Tri Wahyudi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	16s-rrna, marine, cellulase, laccase, sponge-associated bacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6d991a3431c14eb5a476b3903c68664d
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spelling	oai:doaj.org-article:6d991a3431c14eb5a476b3903c68664d2021-11-22T00:28:56ZScreening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes1412-033X2085-472210.13057/biodiv/d210317https://doaj.org/article/6d991a3431c14eb5a476b3903c68664d2020-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5098https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Maharsiwi W, Astuti RI, Meryandini A, Wahyudi AT. 2020. Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes. Biodiversitas 21: 975-981. Exploration of new enzymes from an extreme environment is important to improve industrial efficiency. This study aimed to get sponge-associated bacteria from Seribu Island with the capability to produce cellulase and laccase. These enzyme activities were indicated by the clear zones on CMC medium for cellulase and the reddish-brown zone on Guaiacol medium for laccase. About 100 of sponge-associated bacteria have been isolated from 5 marine sponges used SWC and NA modified media. As screened, one isolate (AGN89) could produce both enzymes and 11 isolates could produce cellulase. Quantitative analysis was performed using the DNS method and obtained the activities of 4 best cellulolytic isolates ranged from 0.04-0.06 UmL-1 and 0.70-1.18 UmL-1 in enzyme and specific activities, respectively. Gene-based determination for the isolate producing laccase resulted in a ±1100 bp amplicon fragment which identified as multicopper oxidase family protein. Based on the 16S-rRNA gene, AGN89 and these 4 cellulolytic isolates were identified as Pseudomonas luteola strain NBRC 103146, Bacillus aerius strain 24k, Pseudomonas aeruginosa strain DSM 50071, Mycobacterium maritypicum strain DSM 20578, and Brachybacterium conglomeratum strain J 1015. This result suggests that the sponge-associated bacteria from Seribu Island could become new enzymes producer for further applications in industry.WENANG MAHARSIWIRIKA INDRI ASTUTIANJA MERYANDINIAris Tri WahyudiMBI & UNS Soloarticle16s-rrna, marine, cellulase, laccase, sponge-associated bacteriaBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s-rrna, marine, cellulase, laccase, sponge-associated bacteria Biology (General) QH301-705.5
spellingShingle	16s-rrna, marine, cellulase, laccase, sponge-associated bacteria Biology (General) QH301-705.5 WENANG MAHARSIWI RIKA INDRI ASTUTI ANJA MERYANDINI Aris Tri Wahyudi Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes
description	Abstract. Maharsiwi W, Astuti RI, Meryandini A, Wahyudi AT. 2020. Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes. Biodiversitas 21: 975-981. Exploration of new enzymes from an extreme environment is important to improve industrial efficiency. This study aimed to get sponge-associated bacteria from Seribu Island with the capability to produce cellulase and laccase. These enzyme activities were indicated by the clear zones on CMC medium for cellulase and the reddish-brown zone on Guaiacol medium for laccase. About 100 of sponge-associated bacteria have been isolated from 5 marine sponges used SWC and NA modified media. As screened, one isolate (AGN89) could produce both enzymes and 11 isolates could produce cellulase. Quantitative analysis was performed using the DNS method and obtained the activities of 4 best cellulolytic isolates ranged from 0.04-0.06 UmL-1 and 0.70-1.18 UmL-1 in enzyme and specific activities, respectively. Gene-based determination for the isolate producing laccase resulted in a ±1100 bp amplicon fragment which identified as multicopper oxidase family protein. Based on the 16S-rRNA gene, AGN89 and these 4 cellulolytic isolates were identified as Pseudomonas luteola strain NBRC 103146, Bacillus aerius strain 24k, Pseudomonas aeruginosa strain DSM 50071, Mycobacterium maritypicum strain DSM 20578, and Brachybacterium conglomeratum strain J 1015. This result suggests that the sponge-associated bacteria from Seribu Island could become new enzymes producer for further applications in industry.
format	article
author	WENANG MAHARSIWI RIKA INDRI ASTUTI ANJA MERYANDINI Aris Tri Wahyudi
author_facet	WENANG MAHARSIWI RIKA INDRI ASTUTI ANJA MERYANDINI Aris Tri Wahyudi
author_sort	WENANG MAHARSIWI
title	Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes
title_short	Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes
title_full	Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes
title_fullStr	Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes
title_full_unstemmed	Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes
title_sort	screening and characterization of sponge-associated bacteria from seribu island, indonesia producing cellulase and laccase enzymes
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/6d991a3431c14eb5a476b3903c68664d
work_keys_str_mv	AT wenangmaharsiwi screeningandcharacterizationofspongeassociatedbacteriafromseribuislandindonesiaproducingcellulaseandlaccaseenzymes AT rikaindriastuti screeningandcharacterizationofspongeassociatedbacteriafromseribuislandindonesiaproducingcellulaseandlaccaseenzymes AT anjameryandini screeningandcharacterizationofspongeassociatedbacteriafromseribuislandindonesiaproducingcellulaseandlaccaseenzymes AT aristriwahyudi screeningandcharacterizationofspongeassociatedbacteriafromseribuislandindonesiaproducingcellulaseandlaccaseenzymes
_version_	1718418587207598080

Screening and characterization of sponge-associated bacteria from Seribu Island, Indonesia producing cellulase and laccase enzymes

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