Salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry

Objectives: Ovulation is such a critical physiological process that its noninvasive detection based on salivary constituents has several advantages in humans. Hence, the present study is proposed to identify the ovulatory-specific proteins in saliva in order to detect ovulation phase.Materials and M...

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Autores principales:	Ganesan Saibaba, Durairaj Rajesh, Subramanian Muthukumar, Ganesan Sathiyanarayanan, Archunan Priya Aarthy, Govindaraju Archunan
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	biomarker cystatin gel electrophoresis liquid chromatography-mass spectrometry/mass spectrometry ovulation protein saliva sodium dodecyl sulfate polyacrylamide gel electrophoresis Gynecology and obstetrics RG1-991
Acceso en línea:	https://doaj.org/article/6d998949792f44cbbef5896a20b83282
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spelling	oai:doaj.org-article:6d998949792f44cbbef5896a20b832822021-11-12T10:10:38ZSalivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry2213-307010.4103/GMIT.GMIT_78_20https://doaj.org/article/6d998949792f44cbbef5896a20b832822021-01-01T00:00:00Zhttp://www.e-gmit.com/article.asp?issn=2213-3070;year=2021;volume=10;issue=4;spage=226;epage=234;aulast=Saibabahttps://doaj.org/toc/2213-3070Objectives: Ovulation is such a critical physiological process that its noninvasive detection based on salivary constituents has several advantages in humans. Hence, the present study is proposed to identify the ovulatory-specific proteins in saliva in order to detect ovulation phase.Materials and Methods: Samples were collected from women volunteers. The procedure adopted was approved by the Institutional Human Ethical Committee (DM/2014/101/38), Bharathidasan University. The saliva samples were collected from thirty healthy female volunteers, with a prior written consent. One-way analysis of variance was used to calculate protein concentration and band intensity using SPSS 16 software (SPSS Inc., Cary, NC, USA). The salivary protein expression pattern during different phases of menstrual cycle was analyzed using gel-based high resolution-liquid chromatography-mass spectrometry/mass spectrometry and matrix-assisted laser desorption ionization-time of flight/time of flight. Further, bioinformatics tools were adopted to annotate the proteins identified at various phases of menstrual cycle.Results: As many as 530 proteins showed up in the saliva during ovulatory phase, whereas there were only 251 proteins identified during postovulatory phase. The functional annotation of salivary proteins revealed that the proteins got assigned to the class of “extracellular proteins” which are concerned with regulatory functions. The 16 unique and/or differentially expressed protein spots appeared during ovulatory phase, among which Cystatin-S, Prolactin-inducible protein, Cystatin-A, Cystatin-SN, BPI fold-containing family A member 2, Alpha-tubulin N-acetyltransferase 1, Carbonic anhydrase-6, Protein LEG1 homolog, Hemoglobin subunit beta, and Pancreatic alpha-amylase were identified.Conclusion: Total salivary proteome profile has been listed with respect to various phases of menstrual cycle. Among the protein listed, Cystatin-S offers a biomarker protein and/or indicator of ovulatory phase. However, extensive validation is required before arriving to a candidate bio-marker protein.Ganesan SaibabaDurairaj RajeshSubramanian MuthukumarGanesan SathiyanarayananArchunan Priya AarthyGovindaraju ArchunanWolters Kluwer Medknow Publicationsarticlebiomarkercystatingel electrophoresisliquid chromatography-mass spectrometry/mass spectrometryovulationproteinsalivasodium dodecyl sulfate polyacrylamide gel electrophoresisGynecology and obstetricsRG1-991ENGynecology and Minimally Invasive Therapy, Vol 10, Iss 4, Pp 226-234 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biomarker cystatin gel electrophoresis liquid chromatography-mass spectrometry/mass spectrometry ovulation protein saliva sodium dodecyl sulfate polyacrylamide gel electrophoresis Gynecology and obstetrics RG1-991
spellingShingle	biomarker cystatin gel electrophoresis liquid chromatography-mass spectrometry/mass spectrometry ovulation protein saliva sodium dodecyl sulfate polyacrylamide gel electrophoresis Gynecology and obstetrics RG1-991 Ganesan Saibaba Durairaj Rajesh Subramanian Muthukumar Ganesan Sathiyanarayanan Archunan Priya Aarthy Govindaraju Archunan Salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry
description	Objectives: Ovulation is such a critical physiological process that its noninvasive detection based on salivary constituents has several advantages in humans. Hence, the present study is proposed to identify the ovulatory-specific proteins in saliva in order to detect ovulation phase.Materials and Methods: Samples were collected from women volunteers. The procedure adopted was approved by the Institutional Human Ethical Committee (DM/2014/101/38), Bharathidasan University. The saliva samples were collected from thirty healthy female volunteers, with a prior written consent. One-way analysis of variance was used to calculate protein concentration and band intensity using SPSS 16 software (SPSS Inc., Cary, NC, USA). The salivary protein expression pattern during different phases of menstrual cycle was analyzed using gel-based high resolution-liquid chromatography-mass spectrometry/mass spectrometry and matrix-assisted laser desorption ionization-time of flight/time of flight. Further, bioinformatics tools were adopted to annotate the proteins identified at various phases of menstrual cycle.Results: As many as 530 proteins showed up in the saliva during ovulatory phase, whereas there were only 251 proteins identified during postovulatory phase. The functional annotation of salivary proteins revealed that the proteins got assigned to the class of “extracellular proteins” which are concerned with regulatory functions. The 16 unique and/or differentially expressed protein spots appeared during ovulatory phase, among which Cystatin-S, Prolactin-inducible protein, Cystatin-A, Cystatin-SN, BPI fold-containing family A member 2, Alpha-tubulin N-acetyltransferase 1, Carbonic anhydrase-6, Protein LEG1 homolog, Hemoglobin subunit beta, and Pancreatic alpha-amylase were identified.Conclusion: Total salivary proteome profile has been listed with respect to various phases of menstrual cycle. Among the protein listed, Cystatin-S offers a biomarker protein and/or indicator of ovulatory phase. However, extensive validation is required before arriving to a candidate bio-marker protein.
format	article
author	Ganesan Saibaba Durairaj Rajesh Subramanian Muthukumar Ganesan Sathiyanarayanan Archunan Priya Aarthy Govindaraju Archunan
author_facet	Ganesan Saibaba Durairaj Rajesh Subramanian Muthukumar Ganesan Sathiyanarayanan Archunan Priya Aarthy Govindaraju Archunan
author_sort	Ganesan Saibaba
title	Salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry
title_short	Salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry
title_full	Salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry
title_fullStr	Salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry
title_full_unstemmed	Salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry
title_sort	salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/6d998949792f44cbbef5896a20b83282
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Salivary proteome profile of women during fertile phase of menstrual cycle as characterized by mass spectrometry

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