Rapid single-wavelength lightsheet localization microscopy for clarified tissue

It has been challenging to perform super-resolution imaging in large volumes due to aberrations encountered. Here, the authors combine single-wavelength Bessel lightsheet localization microscopy with tissue clearing techniques and image neurons across the whole brain of adult fruit flies.

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Detalles Bibliográficos
Autores principales: Li-An Chu, Chieh-Han Lu, Shun-Min Yang, Yen-Ting Liu, Kuan-Lin Feng, Yun-Chi Tsai, Wei-Kun Chang, Wen-Cheng Wang, Shu-Wei Chang, Peilin Chen, Ting-Kuo Lee, Yeu-Kuang Hwu, Ann-Shyn Chiang, Bi-Chang Chen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/6d9a7b48ec084346b8e85b93cccb6cfe
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Sumario:It has been challenging to perform super-resolution imaging in large volumes due to aberrations encountered. Here, the authors combine single-wavelength Bessel lightsheet localization microscopy with tissue clearing techniques and image neurons across the whole brain of adult fruit flies.