Rapid single-wavelength lightsheet localization microscopy for clarified tissue

It has been challenging to perform super-resolution imaging in large volumes due to aberrations encountered. Here, the authors combine single-wavelength Bessel lightsheet localization microscopy with tissue clearing techniques and image neurons across the whole brain of adult fruit flies.

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Autores principales: Li-An Chu, Chieh-Han Lu, Shun-Min Yang, Yen-Ting Liu, Kuan-Lin Feng, Yun-Chi Tsai, Wei-Kun Chang, Wen-Cheng Wang, Shu-Wei Chang, Peilin Chen, Ting-Kuo Lee, Yeu-Kuang Hwu, Ann-Shyn Chiang, Bi-Chang Chen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/6d9a7b48ec084346b8e85b93cccb6cfe
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spelling oai:doaj.org-article:6d9a7b48ec084346b8e85b93cccb6cfe2021-12-02T16:58:17ZRapid single-wavelength lightsheet localization microscopy for clarified tissue10.1038/s41467-019-12715-32041-1723https://doaj.org/article/6d9a7b48ec084346b8e85b93cccb6cfe2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12715-3https://doaj.org/toc/2041-1723It has been challenging to perform super-resolution imaging in large volumes due to aberrations encountered. Here, the authors combine single-wavelength Bessel lightsheet localization microscopy with tissue clearing techniques and image neurons across the whole brain of adult fruit flies.Li-An ChuChieh-Han LuShun-Min YangYen-Ting LiuKuan-Lin FengYun-Chi TsaiWei-Kun ChangWen-Cheng WangShu-Wei ChangPeilin ChenTing-Kuo LeeYeu-Kuang HwuAnn-Shyn ChiangBi-Chang ChenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Li-An Chu
Chieh-Han Lu
Shun-Min Yang
Yen-Ting Liu
Kuan-Lin Feng
Yun-Chi Tsai
Wei-Kun Chang
Wen-Cheng Wang
Shu-Wei Chang
Peilin Chen
Ting-Kuo Lee
Yeu-Kuang Hwu
Ann-Shyn Chiang
Bi-Chang Chen
Rapid single-wavelength lightsheet localization microscopy for clarified tissue
description It has been challenging to perform super-resolution imaging in large volumes due to aberrations encountered. Here, the authors combine single-wavelength Bessel lightsheet localization microscopy with tissue clearing techniques and image neurons across the whole brain of adult fruit flies.
format article
author Li-An Chu
Chieh-Han Lu
Shun-Min Yang
Yen-Ting Liu
Kuan-Lin Feng
Yun-Chi Tsai
Wei-Kun Chang
Wen-Cheng Wang
Shu-Wei Chang
Peilin Chen
Ting-Kuo Lee
Yeu-Kuang Hwu
Ann-Shyn Chiang
Bi-Chang Chen
author_facet Li-An Chu
Chieh-Han Lu
Shun-Min Yang
Yen-Ting Liu
Kuan-Lin Feng
Yun-Chi Tsai
Wei-Kun Chang
Wen-Cheng Wang
Shu-Wei Chang
Peilin Chen
Ting-Kuo Lee
Yeu-Kuang Hwu
Ann-Shyn Chiang
Bi-Chang Chen
author_sort Li-An Chu
title Rapid single-wavelength lightsheet localization microscopy for clarified tissue
title_short Rapid single-wavelength lightsheet localization microscopy for clarified tissue
title_full Rapid single-wavelength lightsheet localization microscopy for clarified tissue
title_fullStr Rapid single-wavelength lightsheet localization microscopy for clarified tissue
title_full_unstemmed Rapid single-wavelength lightsheet localization microscopy for clarified tissue
title_sort rapid single-wavelength lightsheet localization microscopy for clarified tissue
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/6d9a7b48ec084346b8e85b93cccb6cfe
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