Seed Cryopreservation and Germination of Rhus glabra and the Critically Endangered Species Rhus michauxii

Rhus michauxii is a perennial rhizomatous shrub native to the southeastern United States that is found mainly in sunny, dry, open rocky or sandy woodlands. Moreover, it is found on ridges or river bluffs in the inner coastal plane and lower piedmont of Virginia, Georgia, and the C...

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Autores principales:	Gerald S. Pullman, Kylie Bucalo, Ron O. Determann, Jennifer M. Cruse-Sanders
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	conservation cryopreservation endangered species micropropagation shoot culture <i>Rhus michauxii</i> Botany QK1-989
Acceso en línea:	https://doaj.org/article/6da005e5c6884252a5363c4759957158
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spelling	oai:doaj.org-article:6da005e5c6884252a5363c47599571582021-11-25T18:44:41ZSeed Cryopreservation and Germination of <i>Rhus glabra</i> and the Critically Endangered Species <i>Rhus michauxii</i>10.3390/plants101122772223-7747https://doaj.org/article/6da005e5c6884252a5363c47599571582021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2277https://doaj.org/toc/2223-7747<i>Rhus michauxii</i> is a perennial rhizomatous shrub native to the southeastern United States that is found mainly in sunny, dry, open rocky or sandy woodlands. Moreover, it is found on ridges or river bluffs in the inner coastal plane and lower piedmont of Virginia, Georgia, and the Carolinas. Habitat conversion to agriculture, suppression of fires, and low reproduction have caused <i>R. michauxii</i> to become rare and it is now federally listed as threatened. Methods are needed to multiply and conserve <i>R. michauxii</i>. Protocols were developed for seed cryopreservation, in vitro germination, and micropropagation for <i>R. glabra</i> and <i>R. michauxii</i>. Seed scarification in concentrated sulfuric acid for 6 h and germination on ½ MS medium resulted in germination up to 96% for control and cryopreserved seeds of <i>R. glabra</i> and 70 and 40% for control and cryopreserved seeds of <i>R. michauxii</i>. Shortly after germination in vitro, young seedlings were established in a greenhouse potting mix providing new plants from the endemic Georgia <i>R. michauxii</i> populations. Several of the findings meet goals within the <i>R. michauxii</i> recovery plan by providing methods for sexual and asexual multiplication and long-term seed storage under cryogenic conditions. The protocols developed will assist in the safeguarding and conservation of dwindling natural <i>R. michauxii</i> populations.Gerald S. PullmanKylie BucaloRon O. DetermannJennifer M. Cruse-SandersMDPI AGarticleconservationcryopreservationendangered speciesmicropropagationshoot culture<i>Rhus michauxii</i>BotanyQK1-989ENPlants, Vol 10, Iss 2277, p 2277 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	conservation cryopreservation endangered species micropropagation shoot culture <i>Rhus michauxii</i> Botany QK1-989
spellingShingle	conservation cryopreservation endangered species micropropagation shoot culture <i>Rhus michauxii</i> Botany QK1-989 Gerald S. Pullman Kylie Bucalo Ron O. Determann Jennifer M. Cruse-Sanders Seed Cryopreservation and Germination of <i>Rhus glabra</i> and the Critically Endangered Species <i>Rhus michauxii</i>
description	<i>Rhus michauxii</i> is a perennial rhizomatous shrub native to the southeastern United States that is found mainly in sunny, dry, open rocky or sandy woodlands. Moreover, it is found on ridges or river bluffs in the inner coastal plane and lower piedmont of Virginia, Georgia, and the Carolinas. Habitat conversion to agriculture, suppression of fires, and low reproduction have caused <i>R. michauxii</i> to become rare and it is now federally listed as threatened. Methods are needed to multiply and conserve <i>R. michauxii</i>. Protocols were developed for seed cryopreservation, in vitro germination, and micropropagation for <i>R. glabra</i> and <i>R. michauxii</i>. Seed scarification in concentrated sulfuric acid for 6 h and germination on ½ MS medium resulted in germination up to 96% for control and cryopreserved seeds of <i>R. glabra</i> and 70 and 40% for control and cryopreserved seeds of <i>R. michauxii</i>. Shortly after germination in vitro, young seedlings were established in a greenhouse potting mix providing new plants from the endemic Georgia <i>R. michauxii</i> populations. Several of the findings meet goals within the <i>R. michauxii</i> recovery plan by providing methods for sexual and asexual multiplication and long-term seed storage under cryogenic conditions. The protocols developed will assist in the safeguarding and conservation of dwindling natural <i>R. michauxii</i> populations.
format	article
author	Gerald S. Pullman Kylie Bucalo Ron O. Determann Jennifer M. Cruse-Sanders
author_facet	Gerald S. Pullman Kylie Bucalo Ron O. Determann Jennifer M. Cruse-Sanders
author_sort	Gerald S. Pullman
title	Seed Cryopreservation and Germination of <i>Rhus glabra</i> and the Critically Endangered Species <i>Rhus michauxii</i>
title_short	Seed Cryopreservation and Germination of <i>Rhus glabra</i> and the Critically Endangered Species <i>Rhus michauxii</i>
title_full	Seed Cryopreservation and Germination of <i>Rhus glabra</i> and the Critically Endangered Species <i>Rhus michauxii</i>
title_fullStr	Seed Cryopreservation and Germination of <i>Rhus glabra</i> and the Critically Endangered Species <i>Rhus michauxii</i>
title_full_unstemmed	Seed Cryopreservation and Germination of <i>Rhus glabra</i> and the Critically Endangered Species <i>Rhus michauxii</i>
title_sort	seed cryopreservation and germination of <i>rhus glabra</i> and the critically endangered species <i>rhus michauxii</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6da005e5c6884252a5363c4759957158
work_keys_str_mv	AT geraldspullman seedcryopreservationandgerminationofirhusglabraiandthecriticallyendangeredspeciesirhusmichauxiii AT kyliebucalo seedcryopreservationandgerminationofirhusglabraiandthecriticallyendangeredspeciesirhusmichauxiii AT ronodetermann seedcryopreservationandgerminationofirhusglabraiandthecriticallyendangeredspeciesirhusmichauxiii AT jennifermcrusesanders seedcryopreservationandgerminationofirhusglabraiandthecriticallyendangeredspeciesirhusmichauxiii
_version_	1718410777320226816

Seed Cryopreservation and Germination of <i>Rhus glabra</i> and the Critically Endangered Species <i>Rhus michauxii</i>

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