Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum

Abstract In this study, a new type of field-effect transistor (FET)-based biosensor is demonstrated to be able to overcome the problem of severe charge-screening effect caused by high ionic strength in solution and detect proteins in physiological environment. Antibody or aptamer-immobilized AlGaN/G...

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Autores principales:	Chia-Ho Chu, Indu Sarangadharan, Abiral Regmi, Yen-Wen Chen, Chen-Pin Hsu, Wen-Hsin Chang, Geng-Yen Lee, Jen-Inn Chyi, Chih-Chen Chen, Shu-Chu Shiesh, Gwo-Bin Lee, Yu-Lin Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6da60cdd46894422bf680ada3a777a0d
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spelling	oai:doaj.org-article:6da60cdd46894422bf680ada3a777a0d2021-12-02T11:52:23ZBeyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum10.1038/s41598-017-05426-62045-2322https://doaj.org/article/6da60cdd46894422bf680ada3a777a0d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05426-6https://doaj.org/toc/2045-2322Abstract In this study, a new type of field-effect transistor (FET)-based biosensor is demonstrated to be able to overcome the problem of severe charge-screening effect caused by high ionic strength in solution and detect proteins in physiological environment. Antibody or aptamer-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) are used to directly detect proteins, including HIV-1 RT, CEA, NT-proBNP and CRP, in 1X PBS (with 1%BSA) or human sera. The samples do not need any dilution or washing process to reduce the ionic strength. The sensor shows high sensitivity and the detection takes only 5 minutes. The designs of the sensor, the methodology of the measurement, and the working mechanism of the sensor are discussed and investigated. A theoretical model is proposed based on the finding of the experiments. This sensor is promising for point-of-care, home healthcare, and mobile diagnostic device.Chia-Ho ChuIndu SarangadharanAbiral RegmiYen-Wen ChenChen-Pin HsuWen-Hsin ChangGeng-Yen LeeJen-Inn ChyiChih-Chen ChenShu-Chu ShieshGwo-Bin LeeYu-Lin WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chia-Ho Chu Indu Sarangadharan Abiral Regmi Yen-Wen Chen Chen-Pin Hsu Wen-Hsin Chang Geng-Yen Lee Jen-Inn Chyi Chih-Chen Chen Shu-Chu Shiesh Gwo-Bin Lee Yu-Lin Wang Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum
description	Abstract In this study, a new type of field-effect transistor (FET)-based biosensor is demonstrated to be able to overcome the problem of severe charge-screening effect caused by high ionic strength in solution and detect proteins in physiological environment. Antibody or aptamer-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) are used to directly detect proteins, including HIV-1 RT, CEA, NT-proBNP and CRP, in 1X PBS (with 1%BSA) or human sera. The samples do not need any dilution or washing process to reduce the ionic strength. The sensor shows high sensitivity and the detection takes only 5 minutes. The designs of the sensor, the methodology of the measurement, and the working mechanism of the sensor are discussed and investigated. A theoretical model is proposed based on the finding of the experiments. This sensor is promising for point-of-care, home healthcare, and mobile diagnostic device.
format	article
author	Chia-Ho Chu Indu Sarangadharan Abiral Regmi Yen-Wen Chen Chen-Pin Hsu Wen-Hsin Chang Geng-Yen Lee Jen-Inn Chyi Chih-Chen Chen Shu-Chu Shiesh Gwo-Bin Lee Yu-Lin Wang
author_facet	Chia-Ho Chu Indu Sarangadharan Abiral Regmi Yen-Wen Chen Chen-Pin Hsu Wen-Hsin Chang Geng-Yen Lee Jen-Inn Chyi Chih-Chen Chen Shu-Chu Shiesh Gwo-Bin Lee Yu-Lin Wang
author_sort	Chia-Ho Chu
title	Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum
title_short	Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum
title_full	Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum
title_fullStr	Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum
title_full_unstemmed	Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum
title_sort	beyond the debye length in high ionic strength solution: direct protein detection with field-effect transistors (fets) in human serum
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6da60cdd46894422bf680ada3a777a0d
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Beyond the Debye length in high ionic strength solution: direct protein detection with field-effect transistors (FETs) in human serum

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