Establishing community-wide DNA barcode references for conserving mangrove forests in China

Abstract Background Mangrove ecosystems have been the focus of global attention for their crucial role in sheltering coastal communities and retarding global climate change by sequestering ‘blue carbon’. China is relatively rich in mangrove diversity, with one-third of the ca. 70 true mangrove speci...

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Autores principales:	Xiaomeng Mao, Wei Xie, Xinnian Li, Suhua Shi, Zixiao Guo
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Mangroves DNA barcoding Genetic diversity ITS2 rbcL trnH-psbA Botany QK1-989
Acceso en línea:	https://doaj.org/article/6db654341dd045758ff64133d0ee2076
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spelling	oai:doaj.org-article:6db654341dd045758ff64133d0ee20762021-12-05T12:06:17ZEstablishing community-wide DNA barcode references for conserving mangrove forests in China10.1186/s12870-021-03349-z1471-2229https://doaj.org/article/6db654341dd045758ff64133d0ee20762021-12-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03349-zhttps://doaj.org/toc/1471-2229Abstract Background Mangrove ecosystems have been the focus of global attention for their crucial role in sheltering coastal communities and retarding global climate change by sequestering ‘blue carbon’. China is relatively rich in mangrove diversity, with one-third of the ca. 70 true mangrove species and a number of mangrove associate species occurring naturally along the country’s coasts. Mangrove ecosystems, however, are widely threatened by intensifying human disturbances and rising sea levels. DNA barcoding technology may help protect mangrove ecosystems by providing rapid species identification. Results To investigate this potential, 898 plant specimens were collected from 33 major mangrove sites in China. Based on the morphologic diagnosis, the specimens were assigned to 72 species, including all 28 true mangrove species and all 12 mangrove associate species recorded in China. Three chloroplast DNA markers rbcL, trnH-psbA, matK, and one nuclear marker ITS2 were chosen to investigate the utility of using barcoding to identify these species. According to the criteria of barcoding gaps in genetic distance, sequence similarity, and phylogenetic monophyly, we propose that a single marker, ITS2, is sufficient to barcode the species of mangroves and their associates in China. Furthermore, rbcL or trnH-psbA can also be used to gather supplement confirming data. In using these barcodes, we revealed a very low level of genetic variation among geographic locations in the mangrove species, which is an alert to their vulnerability to climate and anthropogenic disturbances. Conclusion We suggest using ITS2 to barcode mangrove species and terrestrial coastal plants in South China. The DNA barcode sequences we obtained would be valuable in monitoring biodiversity and the restoration of ecosystems, which are essential for mangrove conservation.Xiaomeng MaoWei XieXinnian LiSuhua ShiZixiao GuoBMCarticleMangrovesDNA barcodingGenetic diversityITS2rbcLtrnH-psbABotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mangroves DNA barcoding Genetic diversity ITS2 rbcL trnH-psbA Botany QK1-989
spellingShingle	Mangroves DNA barcoding Genetic diversity ITS2 rbcL trnH-psbA Botany QK1-989 Xiaomeng Mao Wei Xie Xinnian Li Suhua Shi Zixiao Guo Establishing community-wide DNA barcode references for conserving mangrove forests in China
description	Abstract Background Mangrove ecosystems have been the focus of global attention for their crucial role in sheltering coastal communities and retarding global climate change by sequestering ‘blue carbon’. China is relatively rich in mangrove diversity, with one-third of the ca. 70 true mangrove species and a number of mangrove associate species occurring naturally along the country’s coasts. Mangrove ecosystems, however, are widely threatened by intensifying human disturbances and rising sea levels. DNA barcoding technology may help protect mangrove ecosystems by providing rapid species identification. Results To investigate this potential, 898 plant specimens were collected from 33 major mangrove sites in China. Based on the morphologic diagnosis, the specimens were assigned to 72 species, including all 28 true mangrove species and all 12 mangrove associate species recorded in China. Three chloroplast DNA markers rbcL, trnH-psbA, matK, and one nuclear marker ITS2 were chosen to investigate the utility of using barcoding to identify these species. According to the criteria of barcoding gaps in genetic distance, sequence similarity, and phylogenetic monophyly, we propose that a single marker, ITS2, is sufficient to barcode the species of mangroves and their associates in China. Furthermore, rbcL or trnH-psbA can also be used to gather supplement confirming data. In using these barcodes, we revealed a very low level of genetic variation among geographic locations in the mangrove species, which is an alert to their vulnerability to climate and anthropogenic disturbances. Conclusion We suggest using ITS2 to barcode mangrove species and terrestrial coastal plants in South China. The DNA barcode sequences we obtained would be valuable in monitoring biodiversity and the restoration of ecosystems, which are essential for mangrove conservation.
format	article
author	Xiaomeng Mao Wei Xie Xinnian Li Suhua Shi Zixiao Guo
author_facet	Xiaomeng Mao Wei Xie Xinnian Li Suhua Shi Zixiao Guo
author_sort	Xiaomeng Mao
title	Establishing community-wide DNA barcode references for conserving mangrove forests in China
title_short	Establishing community-wide DNA barcode references for conserving mangrove forests in China
title_full	Establishing community-wide DNA barcode references for conserving mangrove forests in China
title_fullStr	Establishing community-wide DNA barcode references for conserving mangrove forests in China
title_full_unstemmed	Establishing community-wide DNA barcode references for conserving mangrove forests in China
title_sort	establishing community-wide dna barcode references for conserving mangrove forests in china
publisher	BMC
publishDate	2021
url	https://doaj.org/article/6db654341dd045758ff64133d0ee2076
work_keys_str_mv	AT xiaomengmao establishingcommunitywidednabarcodereferencesforconservingmangroveforestsinchina AT weixie establishingcommunitywidednabarcodereferencesforconservingmangroveforestsinchina AT xinnianli establishingcommunitywidednabarcodereferencesforconservingmangroveforestsinchina AT suhuashi establishingcommunitywidednabarcodereferencesforconservingmangroveforestsinchina AT zixiaoguo establishingcommunitywidednabarcodereferencesforconservingmangroveforestsinchina
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Establishing community-wide DNA barcode references for conserving mangrove forests in China

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