Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography

Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography

Abstract Optical diffraction tomography (ODT) enables imaging of unlabeled intracellular components by measuring the three-dimensional (3D) refractive index (RI). We aimed to detect intracellular monosodium urate (MSU) crystals in synovial leukocytes derived from gout patients using ODT. The 3D RI v...

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Autores principales: Sangwoo Park, Lucy Eunju Lee, Hanna Kim, Ji Eun Kim, Seung Jun Lee, Sunggyu Yoon, Seungwoo Shin, Heemin Kang, YongKeun Park, Jason Jungsik Song, Seongsoo Lee
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Science
Q
Acceso en línea:https://doaj.org/article/6dca21b9171d472a882f4b6f51d445b5
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spelling oai:doaj.org-article:6dca21b9171d472a882f4b6f51d445b52021-12-02T16:58:20ZDetection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography10.1038/s41598-021-89337-72045-2322https://doaj.org/article/6dca21b9171d472a882f4b6f51d445b52021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89337-7https://doaj.org/toc/2045-2322Abstract Optical diffraction tomography (ODT) enables imaging of unlabeled intracellular components by measuring the three-dimensional (3D) refractive index (RI). We aimed to detect intracellular monosodium urate (MSU) crystals in synovial leukocytes derived from gout patients using ODT. The 3D RI values of the synthetic MSU crystals, measured by ODT, ranged between 1.383 and 1.440. After adding synthetic MSU crystals to a macrophage, RI tomograms were reconstructed using ODT, and the reconstructed RI tomograms discerned intracellular and extracellular MSU crystals. We observed unlabeled synthetic MSU crystal entry into the cytoplasm of a macrophage through time-lapse imaging. Furthermore, using gout patient-derived synovial leukocytes, we successfully obtained RI tomogram images of intracellular MSU crystals. The 3D RI identification of MSU crystals was verified with birefringence through polarization-sensitive ODT measurements. Together, our results provide evidence that this novel ODT can identify birefringent MSU crystals in synovial leukocytes of patients with gout.Sangwoo ParkLucy Eunju LeeHanna KimJi Eun KimSeung Jun LeeSunggyu YoonSeungwoo ShinHeemin KangYongKeun ParkJason Jungsik SongSeongsoo LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Sangwoo Park
Lucy Eunju Lee
Hanna Kim
Ji Eun Kim
Seung Jun Lee
Sunggyu Yoon
Seungwoo Shin
Heemin Kang
YongKeun Park
Jason Jungsik Song
Seongsoo Lee
Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography
description Abstract Optical diffraction tomography (ODT) enables imaging of unlabeled intracellular components by measuring the three-dimensional (3D) refractive index (RI). We aimed to detect intracellular monosodium urate (MSU) crystals in synovial leukocytes derived from gout patients using ODT. The 3D RI values of the synthetic MSU crystals, measured by ODT, ranged between 1.383 and 1.440. After adding synthetic MSU crystals to a macrophage, RI tomograms were reconstructed using ODT, and the reconstructed RI tomograms discerned intracellular and extracellular MSU crystals. We observed unlabeled synthetic MSU crystal entry into the cytoplasm of a macrophage through time-lapse imaging. Furthermore, using gout patient-derived synovial leukocytes, we successfully obtained RI tomogram images of intracellular MSU crystals. The 3D RI identification of MSU crystals was verified with birefringence through polarization-sensitive ODT measurements. Together, our results provide evidence that this novel ODT can identify birefringent MSU crystals in synovial leukocytes of patients with gout.
format article
author Sangwoo Park
Lucy Eunju Lee
Hanna Kim
Ji Eun Kim
Seung Jun Lee
Sunggyu Yoon
Seungwoo Shin
Heemin Kang
YongKeun Park
Jason Jungsik Song
Seongsoo Lee
author_facet Sangwoo Park
Lucy Eunju Lee
Hanna Kim
Ji Eun Kim
Seung Jun Lee
Sunggyu Yoon
Seungwoo Shin
Heemin Kang
YongKeun Park
Jason Jungsik Song
Seongsoo Lee
author_sort Sangwoo Park
title Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography
title_short Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography
title_full Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography
title_fullStr Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography
title_full_unstemmed Detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography
title_sort detection of intracellular monosodium urate crystals in gout synovial fluid using optical diffraction tomography
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/6dca21b9171d472a882f4b6f51d445b5
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