Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors

Abstract Infections by Burkholderia cenocepacia lead to life-threatening disease in immunocompromised individuals, including those living with cystic fibrosis (CF). While genetic variation in various B. cenocepacia strains has been reported, it remains unclear how the chemical environment of CF lung...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Olakunle A. Jaiyesimi, Andrew C. McAvoy, David N. Fogg, Neha Garg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6dcc81b4117f43e08055b06a144a68e5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6dcc81b4117f43e08055b06a144a68e5
record_format	dspace
spelling	oai:doaj.org-article:6dcc81b4117f43e08055b06a144a68e52021-11-08T10:55:37ZMetabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors10.1038/s41598-021-00421-42045-2322https://doaj.org/article/6dcc81b4117f43e08055b06a144a68e52021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00421-4https://doaj.org/toc/2045-2322Abstract Infections by Burkholderia cenocepacia lead to life-threatening disease in immunocompromised individuals, including those living with cystic fibrosis (CF). While genetic variation in various B. cenocepacia strains has been reported, it remains unclear how the chemical environment of CF lung influences the production of small molecule virulence factors by these strains. Here we compare metabolomes of three clinical B. cenocepacia strains in synthetic CF sputum medium (SCFM2) and in a routine laboratory medium (LB), in the presence and absence of the antibiotic trimethoprim. Using a mass spectrometry-based untargeted metabolomics approach, we identify several compound classes which are differentially produced in SCFM2 compared to LB media, including siderophores, antimicrobials, quorum sensing signals, and various lipids. Furthermore, we describe that specific metabolites are induced in the presence of the antibiotic trimethoprim only in SCFM2 when compared to LB. Herein, C13-acyl-homoserine lactone, a quorum sensing signal previously not known to be produced by B. cenocepacia as well as pyochelin-type siderophores were exclusively detected during growth in SCFM2 in the presence of trimethoprim. The comparative metabolomics approach described in this study provides insight into environment-dependent production of secondary metabolites by B. cenocepacia strains and suggests future work which could identify personalized strain-specific regulatory mechanisms involved in production of secondary metabolites. Investigations into whether antibiotics with different mechanisms of action induce similar metabolic alterations will inform development of combination treatments aimed at effective clearance of Burkholderia spp. pathogens.Olakunle A. JaiyesimiAndrew C. McAvoyDavid N. FoggNeha GargNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olakunle A. Jaiyesimi Andrew C. McAvoy David N. Fogg Neha Garg Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors
description	Abstract Infections by Burkholderia cenocepacia lead to life-threatening disease in immunocompromised individuals, including those living with cystic fibrosis (CF). While genetic variation in various B. cenocepacia strains has been reported, it remains unclear how the chemical environment of CF lung influences the production of small molecule virulence factors by these strains. Here we compare metabolomes of three clinical B. cenocepacia strains in synthetic CF sputum medium (SCFM2) and in a routine laboratory medium (LB), in the presence and absence of the antibiotic trimethoprim. Using a mass spectrometry-based untargeted metabolomics approach, we identify several compound classes which are differentially produced in SCFM2 compared to LB media, including siderophores, antimicrobials, quorum sensing signals, and various lipids. Furthermore, we describe that specific metabolites are induced in the presence of the antibiotic trimethoprim only in SCFM2 when compared to LB. Herein, C13-acyl-homoserine lactone, a quorum sensing signal previously not known to be produced by B. cenocepacia as well as pyochelin-type siderophores were exclusively detected during growth in SCFM2 in the presence of trimethoprim. The comparative metabolomics approach described in this study provides insight into environment-dependent production of secondary metabolites by B. cenocepacia strains and suggests future work which could identify personalized strain-specific regulatory mechanisms involved in production of secondary metabolites. Investigations into whether antibiotics with different mechanisms of action induce similar metabolic alterations will inform development of combination treatments aimed at effective clearance of Burkholderia spp. pathogens.
format	article
author	Olakunle A. Jaiyesimi Andrew C. McAvoy David N. Fogg Neha Garg
author_facet	Olakunle A. Jaiyesimi Andrew C. McAvoy David N. Fogg Neha Garg
author_sort	Olakunle A. Jaiyesimi
title	Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors
title_short	Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors
title_full	Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors
title_fullStr	Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors
title_full_unstemmed	Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors
title_sort	metabolomic profiling of burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6dcc81b4117f43e08055b06a144a68e5
work_keys_str_mv	AT olakunleajaiyesimi metabolomicprofilingofburkholderiacenocepaciainsyntheticcysticfibrosissputummediumrevealsnutrientenvironmentspecificproductionofvirulencefactors AT andrewcmcavoy metabolomicprofilingofburkholderiacenocepaciainsyntheticcysticfibrosissputummediumrevealsnutrientenvironmentspecificproductionofvirulencefactors AT davidnfogg metabolomicprofilingofburkholderiacenocepaciainsyntheticcysticfibrosissputummediumrevealsnutrientenvironmentspecificproductionofvirulencefactors AT nehagarg metabolomicprofilingofburkholderiacenocepaciainsyntheticcysticfibrosissputummediumrevealsnutrientenvironmentspecificproductionofvirulencefactors
_version_	1718442534017957888

Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors

Ejemplares similares