Evaluation of an Antibody Detecting Point of Care Test for Diagnosis of <i>Taenia solium</i> Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study

The lack of cheap, easy-to-use, rapid diagnostic tests has led to the development of several rapid diagnostic tests for cysticercosis. The new prototype two-strip, <i>Taenia solium</i> point of care test (TS POC) detects antibodies against taeniosis (TS POC T) and cysticercosis (TS POC C...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chishimba Mubanga, Inge Van Damme, Chiara Trevisan, Veronika Schmidt, Isaac K. Phiri, Gideon Zulu, John Noh, Sukwan Handali, Richard Mambo, Mwelwa Chembensofu, Maxwell Masuku, Dries Reynders, Famke Jansen, Emmanuel Bottieau, Pascal Magnussen, Andrea S. Winkler, Pierre Dorny, Kabemba E. Mwape, Sarah Gabriël
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Taenia solium</i> human cysticercosis point of care test diagnosis screening antigen ELISA Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6dd3eb62c73e475297336f095b8a6a38
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6dd3eb62c73e475297336f095b8a6a38
record_format	dspace
spelling	oai:doaj.org-article:6dd3eb62c73e475297336f095b8a6a382021-11-25T17:21:48ZEvaluation of an Antibody Detecting Point of Care Test for Diagnosis of <i>Taenia solium</i> Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study10.3390/diagnostics111121212075-4418https://doaj.org/article/6dd3eb62c73e475297336f095b8a6a382021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/2121https://doaj.org/toc/2075-4418The lack of cheap, easy-to-use, rapid diagnostic tests has led to the development of several rapid diagnostic tests for cysticercosis. The new prototype two-strip, <i>Taenia solium</i> point of care test (TS POC) detects antibodies against taeniosis (TS POC T) and cysticercosis (TS POC CC). This study evaluated the diagnostic performance of the TS POC CC in the Sinda district in eastern Zambia. A sample of 1254 participants was recruited and tested with the TS POC. Out of the 1249 participants with a valid TS POC result, 177 (14%) tested positive while 1072 (86%) tested negative. All individuals with a positive TS POC and a subset of negative TS POC participants were selected for serum sampling, and were subjected to the recombinant glycoprotein T24H enzyme-linked immunoelectrotransfer blot (rT24H EITB) and the serum B60/158 (serum Ag) enzyme-linked immunosorbent assay (Ag ELISA). Performance characteristics were estimated using a Bayesian approach with probabilistic constraints. Based on 255 complete cases, the estimated sensitivity and specificity of the TS POC CC test were 35% (95% CI: 14–63%) and 87% (95% CI: 83–90%), respectively. The diagnostic performance needs to be improved, possibly by titrating antigen and other reagents’ concentration in the strip to produce a performance similar to existing cysticercosis tests such as the rT24H EITB.Chishimba MubangaInge Van DammeChiara TrevisanVeronika SchmidtIsaac K. PhiriGideon ZuluJohn NohSukwan HandaliRichard MamboMwelwa ChembensofuMaxwell MasukuDries ReyndersFamke JansenEmmanuel BottieauPascal MagnussenAndrea S. WinklerPierre DornyKabemba E. MwapeSarah GabriëlMDPI AGarticle<i>Taenia solium</i>human cysticercosispoint of care testdiagnosisscreeningantigen ELISAMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 2121, p 2121 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Taenia solium</i> human cysticercosis point of care test diagnosis screening antigen ELISA Medicine (General) R5-920
spellingShingle	<i>Taenia solium</i> human cysticercosis point of care test diagnosis screening antigen ELISA Medicine (General) R5-920 Chishimba Mubanga Inge Van Damme Chiara Trevisan Veronika Schmidt Isaac K. Phiri Gideon Zulu John Noh Sukwan Handali Richard Mambo Mwelwa Chembensofu Maxwell Masuku Dries Reynders Famke Jansen Emmanuel Bottieau Pascal Magnussen Andrea S. Winkler Pierre Dorny Kabemba E. Mwape Sarah Gabriël Evaluation of an Antibody Detecting Point of Care Test for Diagnosis of <i>Taenia solium</i> Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study
description	The lack of cheap, easy-to-use, rapid diagnostic tests has led to the development of several rapid diagnostic tests for cysticercosis. The new prototype two-strip, <i>Taenia solium</i> point of care test (TS POC) detects antibodies against taeniosis (TS POC T) and cysticercosis (TS POC CC). This study evaluated the diagnostic performance of the TS POC CC in the Sinda district in eastern Zambia. A sample of 1254 participants was recruited and tested with the TS POC. Out of the 1249 participants with a valid TS POC result, 177 (14%) tested positive while 1072 (86%) tested negative. All individuals with a positive TS POC and a subset of negative TS POC participants were selected for serum sampling, and were subjected to the recombinant glycoprotein T24H enzyme-linked immunoelectrotransfer blot (rT24H EITB) and the serum B60/158 (serum Ag) enzyme-linked immunosorbent assay (Ag ELISA). Performance characteristics were estimated using a Bayesian approach with probabilistic constraints. Based on 255 complete cases, the estimated sensitivity and specificity of the TS POC CC test were 35% (95% CI: 14–63%) and 87% (95% CI: 83–90%), respectively. The diagnostic performance needs to be improved, possibly by titrating antigen and other reagents’ concentration in the strip to produce a performance similar to existing cysticercosis tests such as the rT24H EITB.
format	article
author	Chishimba Mubanga Inge Van Damme Chiara Trevisan Veronika Schmidt Isaac K. Phiri Gideon Zulu John Noh Sukwan Handali Richard Mambo Mwelwa Chembensofu Maxwell Masuku Dries Reynders Famke Jansen Emmanuel Bottieau Pascal Magnussen Andrea S. Winkler Pierre Dorny Kabemba E. Mwape Sarah Gabriël
author_facet	Chishimba Mubanga Inge Van Damme Chiara Trevisan Veronika Schmidt Isaac K. Phiri Gideon Zulu John Noh Sukwan Handali Richard Mambo Mwelwa Chembensofu Maxwell Masuku Dries Reynders Famke Jansen Emmanuel Bottieau Pascal Magnussen Andrea S. Winkler Pierre Dorny Kabemba E. Mwape Sarah Gabriël
author_sort	Chishimba Mubanga
title	Evaluation of an Antibody Detecting Point of Care Test for Diagnosis of <i>Taenia solium</i> Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study
title_short	Evaluation of an Antibody Detecting Point of Care Test for Diagnosis of <i>Taenia solium</i> Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study
title_full	Evaluation of an Antibody Detecting Point of Care Test for Diagnosis of <i>Taenia solium</i> Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study
title_fullStr	Evaluation of an Antibody Detecting Point of Care Test for Diagnosis of <i>Taenia solium</i> Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study
title_full_unstemmed	Evaluation of an Antibody Detecting Point of Care Test for Diagnosis of <i>Taenia solium</i> Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study
title_sort	evaluation of an antibody detecting point of care test for diagnosis of <i>taenia solium</i> cysticercosis in a zambian rural community: a prospective diagnostic accuracy study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6dd3eb62c73e475297336f095b8a6a38
work_keys_str_mv	AT chishimbamubanga evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT ingevandamme evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT chiaratrevisan evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT veronikaschmidt evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT isaackphiri evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT gideonzulu evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT johnnoh evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT sukwanhandali evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT richardmambo evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT mwelwachembensofu evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT maxwellmasuku evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT driesreynders evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT famkejansen evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT emmanuelbottieau evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT pascalmagnussen evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT andreaswinkler evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT pierredorny evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT kabembaemwape evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy AT sarahgabriel evaluationofanantibodydetectingpointofcaretestfordiagnosisofitaeniasoliumicysticercosisinazambianruralcommunityaprospectivediagnosticaccuracystudy
_version_	1718412476418097152

Evaluation of an Antibody Detecting Point of Care Test for Diagnosis of <i>Taenia solium</i> Cysticercosis in a Zambian Rural Community: A Prospective Diagnostic Accuracy Study

Ejemplares similares