Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, MsVDAC, from Medicago sativa Confers Cold and Drought Tolerance to Transgenic Tobacco

Voltage-dependent anion channels (VDACs) are highly conserved proteins that are involved in the translocation of tRNA and play a key role in modulating plant senescence and multiple pathways. However, the functions of VDACs in plants are still poorly understood. Here, a novel <...

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Autores principales:	Mei Yang, Xinhang Duan, Zhaoyu Wang, Hang Yin, Junrui Zang, Kai Zhu, Yumeng Wang, Pan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	alfalfa voltage-dependent anion channels ROS scavenging osmotic homeostasis stress-responsive genes Genetics QH426-470
Acceso en línea:	https://doaj.org/article/6dd626303bb141449c1bcd523c6dc603
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spelling	oai:doaj.org-article:6dd626303bb141449c1bcd523c6dc6032021-11-25T17:41:01ZOverexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, <i>MsVDAC</i>, from <i>Medicago sativa</i> Confers Cold and Drought Tolerance to Transgenic Tobacco10.3390/genes121117062073-4425https://doaj.org/article/6dd626303bb141449c1bcd523c6dc6032021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1706https://doaj.org/toc/2073-4425Voltage-dependent anion channels (VDACs) are highly conserved proteins that are involved in the translocation of tRNA and play a key role in modulating plant senescence and multiple pathways. However, the functions of <i>VDACs</i> in plants are still poorly understood. Here, a novel <i>VDAC</i> gene was isolated and identified from alfalfa (<i>Medicago sativa</i> L.). <i>MsVDAC</i> localized to the mitochondria, and its expression was highest in alfalfa roots and was induced in response to cold, drought and salt treatment. Overexpression of <i>MsVDAC</i> in tobacco significantly increased MDA, GSH, soluble sugars, soluble protein and proline contents under cold and drought stress. However, the activities of SOD and POD decreased in transgenic tobacco under cold stress, while the O<sub>2</sub><sup>-</sup> content increased. Stress-responsive genes including <i>LTP1</i>, <i>ERD10B</i> and <i>Hxk3</i> were upregulated in the transgenic plants under cold and drought stress. However, <i>GAPC</i>, <i>CBL1</i>, <i>BI-1</i>, <i>Cu/ZnSOD</i> and <i>MnSOD</i> were upregulated only in the transgenic tobacco plants under cold stress, and <i>GAPC</i>, <i>CBL1</i>, and <i>BI-1</i> were downregulated under drought stress. These results suggest that <i>MsVDAC</i> provides cold tolerance by regulating ROS scavenging, osmotic homeostasis and stress-responsive gene expression in plants, but the improved drought tolerance via <i>MsVDAC</i> may be mainly due to osmotic homeostasis and stress-responsive genes.Mei YangXinhang DuanZhaoyu WangHang YinJunrui ZangKai ZhuYumeng WangPan ZhangMDPI AGarticlealfalfavoltage-dependent anion channelsROS scavengingosmotic homeostasisstress-responsive genesGeneticsQH426-470ENGenes, Vol 12, Iss 1706, p 1706 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	alfalfa voltage-dependent anion channels ROS scavenging osmotic homeostasis stress-responsive genes Genetics QH426-470
spellingShingle	alfalfa voltage-dependent anion channels ROS scavenging osmotic homeostasis stress-responsive genes Genetics QH426-470 Mei Yang Xinhang Duan Zhaoyu Wang Hang Yin Junrui Zang Kai Zhu Yumeng Wang Pan Zhang Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, <i>MsVDAC</i>, from <i>Medicago sativa</i> Confers Cold and Drought Tolerance to Transgenic Tobacco
description	Voltage-dependent anion channels (VDACs) are highly conserved proteins that are involved in the translocation of tRNA and play a key role in modulating plant senescence and multiple pathways. However, the functions of <i>VDACs</i> in plants are still poorly understood. Here, a novel <i>VDAC</i> gene was isolated and identified from alfalfa (<i>Medicago sativa</i> L.). <i>MsVDAC</i> localized to the mitochondria, and its expression was highest in alfalfa roots and was induced in response to cold, drought and salt treatment. Overexpression of <i>MsVDAC</i> in tobacco significantly increased MDA, GSH, soluble sugars, soluble protein and proline contents under cold and drought stress. However, the activities of SOD and POD decreased in transgenic tobacco under cold stress, while the O<sub>2</sub><sup>-</sup> content increased. Stress-responsive genes including <i>LTP1</i>, <i>ERD10B</i> and <i>Hxk3</i> were upregulated in the transgenic plants under cold and drought stress. However, <i>GAPC</i>, <i>CBL1</i>, <i>BI-1</i>, <i>Cu/ZnSOD</i> and <i>MnSOD</i> were upregulated only in the transgenic tobacco plants under cold stress, and <i>GAPC</i>, <i>CBL1</i>, and <i>BI-1</i> were downregulated under drought stress. These results suggest that <i>MsVDAC</i> provides cold tolerance by regulating ROS scavenging, osmotic homeostasis and stress-responsive gene expression in plants, but the improved drought tolerance via <i>MsVDAC</i> may be mainly due to osmotic homeostasis and stress-responsive genes.
format	article
author	Mei Yang Xinhang Duan Zhaoyu Wang Hang Yin Junrui Zang Kai Zhu Yumeng Wang Pan Zhang
author_facet	Mei Yang Xinhang Duan Zhaoyu Wang Hang Yin Junrui Zang Kai Zhu Yumeng Wang Pan Zhang
author_sort	Mei Yang
title	Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, <i>MsVDAC</i>, from <i>Medicago sativa</i> Confers Cold and Drought Tolerance to Transgenic Tobacco
title_short	Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, <i>MsVDAC</i>, from <i>Medicago sativa</i> Confers Cold and Drought Tolerance to Transgenic Tobacco
title_full	Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, <i>MsVDAC</i>, from <i>Medicago sativa</i> Confers Cold and Drought Tolerance to Transgenic Tobacco
title_fullStr	Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, <i>MsVDAC</i>, from <i>Medicago sativa</i> Confers Cold and Drought Tolerance to Transgenic Tobacco
title_full_unstemmed	Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, <i>MsVDAC</i>, from <i>Medicago sativa</i> Confers Cold and Drought Tolerance to Transgenic Tobacco
title_sort	overexpression of a voltage-dependent anion-selective channel (vdac) protein-encoding gene, <i>msvdac</i>, from <i>medicago sativa</i> confers cold and drought tolerance to transgenic tobacco
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6dd626303bb141449c1bcd523c6dc603
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Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, <i>MsVDAC</i>, from <i>Medicago sativa</i> Confers Cold and Drought Tolerance to Transgenic Tobacco

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