eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae
The eukaryotic translation initiation factor 3 (eIF3) complex consists of essential and non-essential sub-complexes. Non-essential eIF3 complex subunits, such as eIF3e, eIF3j, eIF3k, and eIF3l, modulate stress tolerance and enhance the lifespan of Neurospora crassa and Caenorhabditis elegans. Howeve...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:6dd7d93e364947a9b2e712b42099c4472021-11-30T20:04:01ZeIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae1664-462X10.3389/fpls.2021.748120https://doaj.org/article/6dd7d93e364947a9b2e712b42099c4472021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.748120/fullhttps://doaj.org/toc/1664-462XThe eukaryotic translation initiation factor 3 (eIF3) complex consists of essential and non-essential sub-complexes. Non-essential eIF3 complex subunits, such as eIF3e, eIF3j, eIF3k, and eIF3l, modulate stress tolerance and enhance the lifespan of Neurospora crassa and Caenorhabditis elegans. However, there is limited knowledge of the role of the non-essential eIF3 sub-complex in the pathophysiological development of plant fungal pathogens. Here, we deployed genetic and biochemical techniques to explore the influence of a hypothetical protein containing eIF3k domain in Magnaporthe oryzae Oryzae (MoOeIF3k) on reproduction, hyphae morphogenesis, stress tolerance, and pathogenesis. Also, the targeted disruption of MoOeIF3k suppressed vegetative growth and asexual sporulation in ΔMoOeif3k strains significantly. We demonstrated that MoOeIF3k promotes the initiation and development of the rice blast disease by positively regulating the mobilization and degradation of glycogen, appressorium integrity, host penetration, and colonization during host–pathogen interaction. For the first time, we demonstrated that the eIF3k subunit supports the survival of the blast fungus by suppressing vegetative growth and possibly regulating the conversions and utilization of stored cellular energy reserves under starvation conditions. We also observed that the deletion of MoOeIF3k accelerated ribosomal RNA (rRNA) generation in the ΔMoOeif3k strains with a corresponding increase in total protein output. In summary, this study unravels the pathophysiological significance of eIF3k filamentous fungi. The findings also underscored the need to systematically evaluate the individual subunits of the non-essential eIF3 sub-complex during host–pathogen interaction. Further studies are required to unravel the influence of synergetic coordination between translation and transcriptional regulatory machinery on the pathogenesis of filamentous fungi pathogens.Lili LinLili LinJiaying CaoJiaying CaoAnqiang DuAnqiang DuQiuli AnQiuli AnXiaomin ChenXiaomin ChenShuangshuang YuanShuangshuang YuanWajjiha BatoolWajjiha BatoolAmmarah ShabbirAmmarah ShabbirDongmei ZhangDongmei ZhangZonghua WangZonghua WangZonghua WangJustice NorvienyekuJustice NorvienyekuJustice NorvienyekuFrontiers Media S.A.articleMagnaporthe oryzae Oryzaeribosomal RNA (rRNA)non-essential eIF3 complexappressoriumnutrition starvationPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
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| topic |
Magnaporthe oryzae Oryzae ribosomal RNA (rRNA) non-essential eIF3 complex appressorium nutrition starvation Plant culture SB1-1110 |
| spellingShingle |
Magnaporthe oryzae Oryzae ribosomal RNA (rRNA) non-essential eIF3 complex appressorium nutrition starvation Plant culture SB1-1110 Lili Lin Lili Lin Jiaying Cao Jiaying Cao Anqiang Du Anqiang Du Qiuli An Qiuli An Xiaomin Chen Xiaomin Chen Shuangshuang Yuan Shuangshuang Yuan Wajjiha Batool Wajjiha Batool Ammarah Shabbir Ammarah Shabbir Dongmei Zhang Dongmei Zhang Zonghua Wang Zonghua Wang Zonghua Wang Justice Norvienyeku Justice Norvienyeku Justice Norvienyeku eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae |
| description |
The eukaryotic translation initiation factor 3 (eIF3) complex consists of essential and non-essential sub-complexes. Non-essential eIF3 complex subunits, such as eIF3e, eIF3j, eIF3k, and eIF3l, modulate stress tolerance and enhance the lifespan of Neurospora crassa and Caenorhabditis elegans. However, there is limited knowledge of the role of the non-essential eIF3 sub-complex in the pathophysiological development of plant fungal pathogens. Here, we deployed genetic and biochemical techniques to explore the influence of a hypothetical protein containing eIF3k domain in Magnaporthe oryzae Oryzae (MoOeIF3k) on reproduction, hyphae morphogenesis, stress tolerance, and pathogenesis. Also, the targeted disruption of MoOeIF3k suppressed vegetative growth and asexual sporulation in ΔMoOeif3k strains significantly. We demonstrated that MoOeIF3k promotes the initiation and development of the rice blast disease by positively regulating the mobilization and degradation of glycogen, appressorium integrity, host penetration, and colonization during host–pathogen interaction. For the first time, we demonstrated that the eIF3k subunit supports the survival of the blast fungus by suppressing vegetative growth and possibly regulating the conversions and utilization of stored cellular energy reserves under starvation conditions. We also observed that the deletion of MoOeIF3k accelerated ribosomal RNA (rRNA) generation in the ΔMoOeif3k strains with a corresponding increase in total protein output. In summary, this study unravels the pathophysiological significance of eIF3k filamentous fungi. The findings also underscored the need to systematically evaluate the individual subunits of the non-essential eIF3 sub-complex during host–pathogen interaction. Further studies are required to unravel the influence of synergetic coordination between translation and transcriptional regulatory machinery on the pathogenesis of filamentous fungi pathogens. |
| format |
article |
| author |
Lili Lin Lili Lin Jiaying Cao Jiaying Cao Anqiang Du Anqiang Du Qiuli An Qiuli An Xiaomin Chen Xiaomin Chen Shuangshuang Yuan Shuangshuang Yuan Wajjiha Batool Wajjiha Batool Ammarah Shabbir Ammarah Shabbir Dongmei Zhang Dongmei Zhang Zonghua Wang Zonghua Wang Zonghua Wang Justice Norvienyeku Justice Norvienyeku Justice Norvienyeku |
| author_facet |
Lili Lin Lili Lin Jiaying Cao Jiaying Cao Anqiang Du Anqiang Du Qiuli An Qiuli An Xiaomin Chen Xiaomin Chen Shuangshuang Yuan Shuangshuang Yuan Wajjiha Batool Wajjiha Batool Ammarah Shabbir Ammarah Shabbir Dongmei Zhang Dongmei Zhang Zonghua Wang Zonghua Wang Zonghua Wang Justice Norvienyeku Justice Norvienyeku Justice Norvienyeku |
| author_sort |
Lili Lin |
| title |
eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae |
| title_short |
eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae |
| title_full |
eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae |
| title_fullStr |
eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae |
| title_full_unstemmed |
eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae |
| title_sort |
eif3k domain-containing protein regulates conidiogenesis, appressorium turgor, virulence, stress tolerance, and physiological and pathogenic development of magnaporthe oryzae oryzae |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/6dd7d93e364947a9b2e712b42099c447 |
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