Diffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.

We assessed brain abnormalities in rats exposed prenatally to radiation (X-rays) using magnetic resonance imaging (MRI) and histological experiments. Pregnant rats were divided into 4 groups: the control group (n = 3) and 3 groups that were exposed to different radiation doses (0.5, 1.0, or 1.5 Gy;...

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Autores principales:	Shigeyoshi Saito, Kazuhiko Sawada, Miwa Hirose, Yuki Mori, Yoshichika Yoshioka, Kenya Murase
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6dddd3dcf1994ea0a9d174ea1b43b90a
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spelling	oai:doaj.org-article:6dddd3dcf1994ea0a9d174ea1b43b90a2021-11-25T06:01:21ZDiffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.1932-620310.1371/journal.pone.0107368https://doaj.org/article/6dddd3dcf1994ea0a9d174ea1b43b90a2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107368https://doaj.org/toc/1932-6203We assessed brain abnormalities in rats exposed prenatally to radiation (X-rays) using magnetic resonance imaging (MRI) and histological experiments. Pregnant rats were divided into 4 groups: the control group (n = 3) and 3 groups that were exposed to different radiation doses (0.5, 1.0, or 1.5 Gy; n = 3 each). Brain abnormalities were assessed in 32 neonatal male rats (8 per group). Ex vivo T2-weighted imaging and diffusion tensor imaging (DTI) were performed using 11.7-T MRI. The expression of markers of myelin production (Kluver-Barrera staining, KB), nonpyramidal cells (calbindin-D28k staining, CaBP), and pyramidal cells (staining of the nonphosphorylated heavy-chain neurofilament SMI-32) were histologically evaluated. Decreased brain volume, increased ventricle volume, and thinner cortices were observed by MRI in irradiated rats. However, no abnormalities in the cortical 6-layered structure were observed via KB staining in radiation-exposed rats. The DTI color-coded map revealed a dose-dependent reduction in the anisotropic signal (vertical direction), which did not represent reduced numbers of pyramidal cells; rather, it indicated a signal reduction relative to the vertical direction because of low nerve cell density in the entire cortex. We conclude that DTI and histological experiments are useful tools for assessing cortical and hippocampal abnormalities after prenatal exposure to radiation in rats.Shigeyoshi SaitoKazuhiko SawadaMiwa HiroseYuki MoriYoshichika YoshiokaKenya MurasePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107368 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shigeyoshi Saito Kazuhiko Sawada Miwa Hirose Yuki Mori Yoshichika Yoshioka Kenya Murase Diffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.
description	We assessed brain abnormalities in rats exposed prenatally to radiation (X-rays) using magnetic resonance imaging (MRI) and histological experiments. Pregnant rats were divided into 4 groups: the control group (n = 3) and 3 groups that were exposed to different radiation doses (0.5, 1.0, or 1.5 Gy; n = 3 each). Brain abnormalities were assessed in 32 neonatal male rats (8 per group). Ex vivo T2-weighted imaging and diffusion tensor imaging (DTI) were performed using 11.7-T MRI. The expression of markers of myelin production (Kluver-Barrera staining, KB), nonpyramidal cells (calbindin-D28k staining, CaBP), and pyramidal cells (staining of the nonphosphorylated heavy-chain neurofilament SMI-32) were histologically evaluated. Decreased brain volume, increased ventricle volume, and thinner cortices were observed by MRI in irradiated rats. However, no abnormalities in the cortical 6-layered structure were observed via KB staining in radiation-exposed rats. The DTI color-coded map revealed a dose-dependent reduction in the anisotropic signal (vertical direction), which did not represent reduced numbers of pyramidal cells; rather, it indicated a signal reduction relative to the vertical direction because of low nerve cell density in the entire cortex. We conclude that DTI and histological experiments are useful tools for assessing cortical and hippocampal abnormalities after prenatal exposure to radiation in rats.
format	article
author	Shigeyoshi Saito Kazuhiko Sawada Miwa Hirose Yuki Mori Yoshichika Yoshioka Kenya Murase
author_facet	Shigeyoshi Saito Kazuhiko Sawada Miwa Hirose Yuki Mori Yoshichika Yoshioka Kenya Murase
author_sort	Shigeyoshi Saito
title	Diffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.
title_short	Diffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.
title_full	Diffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.
title_fullStr	Diffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.
title_full_unstemmed	Diffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.
title_sort	diffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/6dddd3dcf1994ea0a9d174ea1b43b90a
work_keys_str_mv	AT shigeyoshisaito diffusiontensorimagingofbrainabnormalitiesinducedbyprenatalexposuretoradiationinrodents AT kazuhikosawada diffusiontensorimagingofbrainabnormalitiesinducedbyprenatalexposuretoradiationinrodents AT miwahirose diffusiontensorimagingofbrainabnormalitiesinducedbyprenatalexposuretoradiationinrodents AT yukimori diffusiontensorimagingofbrainabnormalitiesinducedbyprenatalexposuretoradiationinrodents AT yoshichikayoshioka diffusiontensorimagingofbrainabnormalitiesinducedbyprenatalexposuretoradiationinrodents AT kenyamurase diffusiontensorimagingofbrainabnormalitiesinducedbyprenatalexposuretoradiationinrodents
_version_	1718414288337502208

Diffusion tensor imaging of brain abnormalities induced by prenatal exposure to radiation in rodents.

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