True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy

Methods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with high sensitivity and throughput.

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Autores principales:	Christophe Jung, Peter Bandilla, Marc von Reutern, Max Schnepf, Susanne Rieder, Ulrich Unnerstall, Ulrike Gaul
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6de05cf9c14c415c94eeb469a7b93565
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spelling	oai:doaj.org-article:6de05cf9c14c415c94eeb469a7b935652021-12-02T15:33:41ZTrue equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy10.1038/s41467-018-03977-42041-1723https://doaj.org/article/6de05cf9c14c415c94eeb469a7b935652018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03977-4https://doaj.org/toc/2041-1723Methods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with high sensitivity and throughput.Christophe JungPeter BandillaMarc von ReuternMax SchnepfSusanne RiederUlrich UnnerstallUlrike GaulNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Christophe Jung Peter Bandilla Marc von Reutern Max Schnepf Susanne Rieder Ulrich Unnerstall Ulrike Gaul True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
description	Methods to measure selective transcription factor-DNA binding often lack sensitivity and are not performed in solution. Here the authors develop a method to perform fluorescence anisotropy measurements of transcription factor-DNA binding energies with high sensitivity and throughput.
format	article
author	Christophe Jung Peter Bandilla Marc von Reutern Max Schnepf Susanne Rieder Ulrich Unnerstall Ulrike Gaul
author_facet	Christophe Jung Peter Bandilla Marc von Reutern Max Schnepf Susanne Rieder Ulrich Unnerstall Ulrike Gaul
author_sort	Christophe Jung
title	True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_short	True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_full	True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_fullStr	True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_full_unstemmed	True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy
title_sort	true equilibrium measurement of transcription factor-dna binding affinities using automated polarization microscopy
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6de05cf9c14c415c94eeb469a7b93565
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True equilibrium measurement of transcription factor-DNA binding affinities using automated polarization microscopy

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