Slowdown of Translational Elongation in <italic toggle="yes">Escherichia coli</italic> under Hyperosmotic Stress

ABSTRACT In nature, bacteria frequently experience many adverse conditions, including heat, oxidation, acidity, and hyperosmolarity, which all tend to slow down if not outright stop cell growth. Previous work on bacterial stress mainly focused on understanding gene regulatory responses. Much less is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiongfeng Dai, Manlu Zhu, Mya Warren, Rohan Balakrishnan, Hiroyuki Okano, James R. Williamson, Kurt Fredrick, Terence Hwa
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	hyperosmotic stress protein synthesis ribosome content translational elongation rate Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6ded3cae351240e9885173243923a252
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6ded3cae351240e9885173243923a252
record_format	dspace
spelling	oai:doaj.org-article:6ded3cae351240e9885173243923a2522021-11-15T15:53:25ZSlowdown of Translational Elongation in <italic toggle="yes">Escherichia coli</italic> under Hyperosmotic Stress10.1128/mBio.02375-172150-7511https://doaj.org/article/6ded3cae351240e9885173243923a2522018-03-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02375-17https://doaj.org/toc/2150-7511ABSTRACT In nature, bacteria frequently experience many adverse conditions, including heat, oxidation, acidity, and hyperosmolarity, which all tend to slow down if not outright stop cell growth. Previous work on bacterial stress mainly focused on understanding gene regulatory responses. Much less is known about how stresses compromise protein synthesis, which is the major driver of cell growth. Here, we quantitatively characterize the translational capacity of Escherichia coli cells growing exponentially under hyperosmotic stress. We found that hyperosmotic stress affects bacterial protein synthesis through reduction of the translational elongation rate, which is largely compensated for by an increase in the cellular ribosome content compared with nutrient limitation at a similar growth rate. The slowdown of translational elongation is attributed to a reduction in the rate of binding of tRNA ternary complexes to the ribosomes. IMPORTANCE Hyperosmotic stress is a common stress condition confronted by E. coli during infection of the urinary tract. It can significantly compromise the bacterial growth rate. Protein translation capacity is a critical component of bacterial growth. In this study, we find for the first time that hyperosmotic stress causes substantial slowdown in bacterial ribosome translation elongation. The slowdown of translation elongation originates from a reduced binding rate of tRNA ternary complex to the ribosomes.Xiongfeng DaiManlu ZhuMya WarrenRohan BalakrishnanHiroyuki OkanoJames R. WilliamsonKurt FredrickTerence HwaAmerican Society for Microbiologyarticlehyperosmotic stressprotein synthesisribosome contenttranslational elongation rateMicrobiologyQR1-502ENmBio, Vol 9, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	hyperosmotic stress protein synthesis ribosome content translational elongation rate Microbiology QR1-502
spellingShingle	hyperosmotic stress protein synthesis ribosome content translational elongation rate Microbiology QR1-502 Xiongfeng Dai Manlu Zhu Mya Warren Rohan Balakrishnan Hiroyuki Okano James R. Williamson Kurt Fredrick Terence Hwa Slowdown of Translational Elongation in <italic toggle="yes">Escherichia coli</italic> under Hyperosmotic Stress
description	ABSTRACT In nature, bacteria frequently experience many adverse conditions, including heat, oxidation, acidity, and hyperosmolarity, which all tend to slow down if not outright stop cell growth. Previous work on bacterial stress mainly focused on understanding gene regulatory responses. Much less is known about how stresses compromise protein synthesis, which is the major driver of cell growth. Here, we quantitatively characterize the translational capacity of Escherichia coli cells growing exponentially under hyperosmotic stress. We found that hyperosmotic stress affects bacterial protein synthesis through reduction of the translational elongation rate, which is largely compensated for by an increase in the cellular ribosome content compared with nutrient limitation at a similar growth rate. The slowdown of translational elongation is attributed to a reduction in the rate of binding of tRNA ternary complexes to the ribosomes. IMPORTANCE Hyperosmotic stress is a common stress condition confronted by E. coli during infection of the urinary tract. It can significantly compromise the bacterial growth rate. Protein translation capacity is a critical component of bacterial growth. In this study, we find for the first time that hyperosmotic stress causes substantial slowdown in bacterial ribosome translation elongation. The slowdown of translation elongation originates from a reduced binding rate of tRNA ternary complex to the ribosomes.
format	article
author	Xiongfeng Dai Manlu Zhu Mya Warren Rohan Balakrishnan Hiroyuki Okano James R. Williamson Kurt Fredrick Terence Hwa
author_facet	Xiongfeng Dai Manlu Zhu Mya Warren Rohan Balakrishnan Hiroyuki Okano James R. Williamson Kurt Fredrick Terence Hwa
author_sort	Xiongfeng Dai
title	Slowdown of Translational Elongation in <italic toggle="yes">Escherichia coli</italic> under Hyperosmotic Stress
title_short	Slowdown of Translational Elongation in <italic toggle="yes">Escherichia coli</italic> under Hyperosmotic Stress
title_full	Slowdown of Translational Elongation in <italic toggle="yes">Escherichia coli</italic> under Hyperosmotic Stress
title_fullStr	Slowdown of Translational Elongation in <italic toggle="yes">Escherichia coli</italic> under Hyperosmotic Stress
title_full_unstemmed	Slowdown of Translational Elongation in <italic toggle="yes">Escherichia coli</italic> under Hyperosmotic Stress
title_sort	slowdown of translational elongation in <italic toggle="yes">escherichia coli</italic> under hyperosmotic stress
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/6ded3cae351240e9885173243923a252
work_keys_str_mv	AT xiongfengdai slowdownoftranslationalelongationinitalictoggleyesescherichiacoliitalicunderhyperosmoticstress AT manluzhu slowdownoftranslationalelongationinitalictoggleyesescherichiacoliitalicunderhyperosmoticstress AT myawarren slowdownoftranslationalelongationinitalictoggleyesescherichiacoliitalicunderhyperosmoticstress AT rohanbalakrishnan slowdownoftranslationalelongationinitalictoggleyesescherichiacoliitalicunderhyperosmoticstress AT hiroyukiokano slowdownoftranslationalelongationinitalictoggleyesescherichiacoliitalicunderhyperosmoticstress AT jamesrwilliamson slowdownoftranslationalelongationinitalictoggleyesescherichiacoliitalicunderhyperosmoticstress AT kurtfredrick slowdownoftranslationalelongationinitalictoggleyesescherichiacoliitalicunderhyperosmoticstress AT terencehwa slowdownoftranslationalelongationinitalictoggleyesescherichiacoliitalicunderhyperosmoticstress
_version_	1718427280660758528

Slowdown of Translational Elongation in <italic toggle="yes">Escherichia coli</italic> under Hyperosmotic Stress

Ejemplares similares