Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.

A recent large outbreak of fungal infections by Exserohilum rostratum from contaminated compounding solutions has highlighted the need to rapidly screen available pharmaceuticals that could be useful in therapy. The present study utilized two newly-developed high throughput assays to screen approved...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wei Sun, Yoon-Dong Park, Janyce A Sugui, Annette Fothergill, Noel Southall, Paul Shinn, John C McKew, Kyung J Kwon-Chung, Wei Zheng, Peter R Williamson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6df2c262060b48b5a5bb9950eb518e88
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6df2c262060b48b5a5bb9950eb518e88
record_format	dspace
spelling	oai:doaj.org-article:6df2c262060b48b5a5bb9950eb518e882021-11-18T08:58:39ZRapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.1932-620310.1371/journal.pone.0070506https://doaj.org/article/6df2c262060b48b5a5bb9950eb518e882013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23990907/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A recent large outbreak of fungal infections by Exserohilum rostratum from contaminated compounding solutions has highlighted the need to rapidly screen available pharmaceuticals that could be useful in therapy. The present study utilized two newly-developed high throughput assays to screen approved drugs and pharmaceutically active compounds for identification of potential antifungal agents. Several known drugs were found that have potent effects against E. rostratum including the triazole antifungal posaconazole. Posaconazole is likely to be effective against infections involving septic joints and may provide an alternative for refractory central nervous system infections. The anti-E. rostratum activities of several other drugs including bithionol (an anti-parasitic drug), tacrolimus (an immunosuppressive agent) and floxuridine (an antimetabolite) were also identified from the drug repurposing screens. In addition, activities of other potential antifungal agents against E. rostratum were excluded, which may avoid unnecessary therapeutic trials and reveals the limited therapeutic alternatives for this outbreak. In summary, this study has demonstrated that drug repurposing screens can be quickly conducted within a useful time-frame. This would allow clinical implementation of identified alternative therapeutics and should be considered as part of the initial public health response to new outbreaks or rapidly-emerging microbial pathogens.Wei SunYoon-Dong ParkJanyce A SuguiAnnette FothergillNoel SouthallPaul ShinnJohn C McKewKyung J Kwon-ChungWei ZhengPeter R WilliamsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e70506 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Sun Yoon-Dong Park Janyce A Sugui Annette Fothergill Noel Southall Paul Shinn John C McKew Kyung J Kwon-Chung Wei Zheng Peter R Williamson Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.
description	A recent large outbreak of fungal infections by Exserohilum rostratum from contaminated compounding solutions has highlighted the need to rapidly screen available pharmaceuticals that could be useful in therapy. The present study utilized two newly-developed high throughput assays to screen approved drugs and pharmaceutically active compounds for identification of potential antifungal agents. Several known drugs were found that have potent effects against E. rostratum including the triazole antifungal posaconazole. Posaconazole is likely to be effective against infections involving septic joints and may provide an alternative for refractory central nervous system infections. The anti-E. rostratum activities of several other drugs including bithionol (an anti-parasitic drug), tacrolimus (an immunosuppressive agent) and floxuridine (an antimetabolite) were also identified from the drug repurposing screens. In addition, activities of other potential antifungal agents against E. rostratum were excluded, which may avoid unnecessary therapeutic trials and reveals the limited therapeutic alternatives for this outbreak. In summary, this study has demonstrated that drug repurposing screens can be quickly conducted within a useful time-frame. This would allow clinical implementation of identified alternative therapeutics and should be considered as part of the initial public health response to new outbreaks or rapidly-emerging microbial pathogens.
format	article
author	Wei Sun Yoon-Dong Park Janyce A Sugui Annette Fothergill Noel Southall Paul Shinn John C McKew Kyung J Kwon-Chung Wei Zheng Peter R Williamson
author_facet	Wei Sun Yoon-Dong Park Janyce A Sugui Annette Fothergill Noel Southall Paul Shinn John C McKew Kyung J Kwon-Chung Wei Zheng Peter R Williamson
author_sort	Wei Sun
title	Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.
title_short	Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.
title_full	Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.
title_fullStr	Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.
title_full_unstemmed	Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.
title_sort	rapid identification of antifungal compounds against exserohilum rostratum using high throughput drug repurposing screens.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6df2c262060b48b5a5bb9950eb518e88
work_keys_str_mv	AT weisun rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens AT yoondongpark rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens AT janyceasugui rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens AT annettefothergill rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens AT noelsouthall rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens AT paulshinn rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens AT johncmckew rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens AT kyungjkwonchung rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens AT weizheng rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens AT peterrwilliamson rapididentificationofantifungalcompoundsagainstexserohilumrostratumusinghighthroughputdrugrepurposingscreens
_version_	1718421087724765184

Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.

Ejemplares similares