Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide

Abstract Penicillium digitatum is the primary spoilage fungus that causes green mold during postharvest in citrus. To reduce economic losses, developing more efficient and less toxic natural antimicrobial agents is urgently required. We previously found that the X33 antimicrobial oligopeptide (X33 A...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shuhua Lin, Yuanxiu Wang, Qunlin Lu, Bin Zhang, Xiaoyu Wu
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	X33 antimicrobial oligopeptide Penicillium digitatum Antimicrobial mechanism Transcriptomics Metabolomics Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/6dfc35410c6a48dfb4da0d9c54093025
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6dfc35410c6a48dfb4da0d9c54093025
record_format	dspace
spelling	oai:doaj.org-article:6dfc35410c6a48dfb4da0d9c540930252021-12-05T12:03:55ZCombined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide10.1186/s40643-021-00472-52197-4365https://doaj.org/article/6dfc35410c6a48dfb4da0d9c540930252021-12-01T00:00:00Zhttps://doi.org/10.1186/s40643-021-00472-5https://doaj.org/toc/2197-4365Abstract Penicillium digitatum is the primary spoilage fungus that causes green mold during postharvest in citrus. To reduce economic losses, developing more efficient and less toxic natural antimicrobial agents is urgently required. We previously found that the X33 antimicrobial oligopeptide (X33 AMOP), produced by Streptomyces lavendulae X33, exhibited a sterilization effect on P. digitatum. In this study, the effects, and physiological mechanisms of X33 AMOP as an inhibitor of P. digitatum were investigated. The transcriptional and metabolome profiling of P. digitatum exposed to X33 AMOP revealed 3648 genes and 190 metabolites that were prominently changed. The omics analyses suggested that X33 AMOP mainly inhibited P. digitatum growth by affecting cell integrity, genetic information delivery, oxidative stress tolerance, and energy metabolism. These findings provide helpful information regarding the antimicrobial mechanism of X33 AMOP against P. digitatum at the molecular level and indicate that X33 AMOP is a potential candidate to control P. digitatum. Graphical AbstractShuhua LinYuanxiu WangQunlin LuBin ZhangXiaoyu WuSpringerOpenarticleX33 antimicrobial oligopeptidePenicillium digitatumAntimicrobial mechanismTranscriptomicsMetabolomicsTechnologyTChemical technologyTP1-1185BiotechnologyTP248.13-248.65ENBioresources and Bioprocessing, Vol 8, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	X33 antimicrobial oligopeptide Penicillium digitatum Antimicrobial mechanism Transcriptomics Metabolomics Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65
spellingShingle	X33 antimicrobial oligopeptide Penicillium digitatum Antimicrobial mechanism Transcriptomics Metabolomics Technology T Chemical technology TP1-1185 Biotechnology TP248.13-248.65 Shuhua Lin Yuanxiu Wang Qunlin Lu Bin Zhang Xiaoyu Wu Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide
description	Abstract Penicillium digitatum is the primary spoilage fungus that causes green mold during postharvest in citrus. To reduce economic losses, developing more efficient and less toxic natural antimicrobial agents is urgently required. We previously found that the X33 antimicrobial oligopeptide (X33 AMOP), produced by Streptomyces lavendulae X33, exhibited a sterilization effect on P. digitatum. In this study, the effects, and physiological mechanisms of X33 AMOP as an inhibitor of P. digitatum were investigated. The transcriptional and metabolome profiling of P. digitatum exposed to X33 AMOP revealed 3648 genes and 190 metabolites that were prominently changed. The omics analyses suggested that X33 AMOP mainly inhibited P. digitatum growth by affecting cell integrity, genetic information delivery, oxidative stress tolerance, and energy metabolism. These findings provide helpful information regarding the antimicrobial mechanism of X33 AMOP against P. digitatum at the molecular level and indicate that X33 AMOP is a potential candidate to control P. digitatum. Graphical Abstract
format	article
author	Shuhua Lin Yuanxiu Wang Qunlin Lu Bin Zhang Xiaoyu Wu
author_facet	Shuhua Lin Yuanxiu Wang Qunlin Lu Bin Zhang Xiaoyu Wu
author_sort	Shuhua Lin
title	Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide
title_short	Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide
title_full	Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide
title_fullStr	Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide
title_full_unstemmed	Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide
title_sort	combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of penicillium digitatum by x33 antimicrobial oligopeptide
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/6dfc35410c6a48dfb4da0d9c54093025
work_keys_str_mv	AT shuhualin combinedtranscriptomeandmetabolomeanalysesrevealthepotentialmechanismfortheinhibitionofpenicilliumdigitatumbyx33antimicrobialoligopeptide AT yuanxiuwang combinedtranscriptomeandmetabolomeanalysesrevealthepotentialmechanismfortheinhibitionofpenicilliumdigitatumbyx33antimicrobialoligopeptide AT qunlinlu combinedtranscriptomeandmetabolomeanalysesrevealthepotentialmechanismfortheinhibitionofpenicilliumdigitatumbyx33antimicrobialoligopeptide AT binzhang combinedtranscriptomeandmetabolomeanalysesrevealthepotentialmechanismfortheinhibitionofpenicilliumdigitatumbyx33antimicrobialoligopeptide AT xiaoyuwu combinedtranscriptomeandmetabolomeanalysesrevealthepotentialmechanismfortheinhibitionofpenicilliumdigitatumbyx33antimicrobialoligopeptide
_version_	1718372261174444032

Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide

Ejemplares similares