Study of global transcriptional changes of N-GlcNAc2 proteins-producing T24 bladder carcinoma cells under glucose deprivation.

Increased levels of N-linked (β-N- acetylglucosamine)2 [N-GlcNAc2]-modified proteins have been recognized to be an effective response to glucose deprivation. In the first step of this study, using a next generation sequencer, we investigated the global transcriptional changes induced by glucose depr...

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Auteurs principaux:	Takahiro Isono, Tokuhiro Chano, Hidetoshi Okabe, Masafumi Suzaki
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2013
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/6e005975e0484845aa28d86312dd7a55
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spelling	oai:doaj.org-article:6e005975e0484845aa28d86312dd7a552021-11-18T07:51:11ZStudy of global transcriptional changes of N-GlcNAc2 proteins-producing T24 bladder carcinoma cells under glucose deprivation.1932-620310.1371/journal.pone.0060397https://doaj.org/article/6e005975e0484845aa28d86312dd7a552013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23560094/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Increased levels of N-linked (β-N- acetylglucosamine)2 [N-GlcNAc2]-modified proteins have been recognized to be an effective response to glucose deprivation. In the first step of this study, using a next generation sequencer, we investigated the global transcriptional changes induced by glucose deprivation in a T24 bladder carcinoma cell line, producing N-GlcNAc2-modified proteins under glucose deprivation. Our transcriptome analysis revealed significant up-regulation of the UDP-GlcNAc biosynthesis pathway and unfolded protein response genes, and down-regulation of G2/M transition-related genes containing mitotic kinases. Our biological analysis confirmed that N-GlcNAc2-modified proteins were localized with BiP proteins in the ER. G2/M arrest was caused by glucose deprivation in T24 cells. Moreover, the knockdown of unfolded protein response genes induced the expressional recovery of mitotic kinases under glucose deprivation. Taken together, our results suggest N-GlcNAc2-modified proteins produced under glucose deprivation caused unfolded protein response in the ER, and that this response induced G2/M arrest.Takahiro IsonoTokuhiro ChanoHidetoshi OkabeMasafumi SuzakiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60397 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takahiro Isono Tokuhiro Chano Hidetoshi Okabe Masafumi Suzaki Study of global transcriptional changes of N-GlcNAc2 proteins-producing T24 bladder carcinoma cells under glucose deprivation.
description	Increased levels of N-linked (β-N- acetylglucosamine)2 [N-GlcNAc2]-modified proteins have been recognized to be an effective response to glucose deprivation. In the first step of this study, using a next generation sequencer, we investigated the global transcriptional changes induced by glucose deprivation in a T24 bladder carcinoma cell line, producing N-GlcNAc2-modified proteins under glucose deprivation. Our transcriptome analysis revealed significant up-regulation of the UDP-GlcNAc biosynthesis pathway and unfolded protein response genes, and down-regulation of G2/M transition-related genes containing mitotic kinases. Our biological analysis confirmed that N-GlcNAc2-modified proteins were localized with BiP proteins in the ER. G2/M arrest was caused by glucose deprivation in T24 cells. Moreover, the knockdown of unfolded protein response genes induced the expressional recovery of mitotic kinases under glucose deprivation. Taken together, our results suggest N-GlcNAc2-modified proteins produced under glucose deprivation caused unfolded protein response in the ER, and that this response induced G2/M arrest.
format	article
author	Takahiro Isono Tokuhiro Chano Hidetoshi Okabe Masafumi Suzaki
author_facet	Takahiro Isono Tokuhiro Chano Hidetoshi Okabe Masafumi Suzaki
author_sort	Takahiro Isono
title	Study of global transcriptional changes of N-GlcNAc2 proteins-producing T24 bladder carcinoma cells under glucose deprivation.
title_short	Study of global transcriptional changes of N-GlcNAc2 proteins-producing T24 bladder carcinoma cells under glucose deprivation.
title_full	Study of global transcriptional changes of N-GlcNAc2 proteins-producing T24 bladder carcinoma cells under glucose deprivation.
title_fullStr	Study of global transcriptional changes of N-GlcNAc2 proteins-producing T24 bladder carcinoma cells under glucose deprivation.
title_full_unstemmed	Study of global transcriptional changes of N-GlcNAc2 proteins-producing T24 bladder carcinoma cells under glucose deprivation.
title_sort	study of global transcriptional changes of n-glcnac2 proteins-producing t24 bladder carcinoma cells under glucose deprivation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6e005975e0484845aa28d86312dd7a55
work_keys_str_mv	AT takahiroisono studyofglobaltranscriptionalchangesofnglcnac2proteinsproducingt24bladdercarcinomacellsunderglucosedeprivation AT tokuhirochano studyofglobaltranscriptionalchangesofnglcnac2proteinsproducingt24bladdercarcinomacellsunderglucosedeprivation AT hidetoshiokabe studyofglobaltranscriptionalchangesofnglcnac2proteinsproducingt24bladdercarcinomacellsunderglucosedeprivation AT masafumisuzaki studyofglobaltranscriptionalchangesofnglcnac2proteinsproducingt24bladdercarcinomacellsunderglucosedeprivation
_version_	1718422858882875392

Study of global transcriptional changes of N-GlcNAc2 proteins-producing T24 bladder carcinoma cells under glucose deprivation.

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