Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1

ABSTRACT Bacterial viruses, or bacteriophages, are highly abundant in the biosphere and have a major impact on microbial populations. Many examples of phage interactions with their hosts, including establishment of dormant lysogenic and active lytic states, have been characterized at the level of th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Leanid Laganenka, Timur Sander, Alexander Lagonenko, Yu Chen, Hannes Link, Victor Sourjik
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Escherichia coli bacteriophage lysis cyclic AMP quorum sensing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6e05b1b058324b10a609d89d7833c1df
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6e05b1b058324b10a609d89d7833c1df
record_format	dspace
spelling	oai:doaj.org-article:6e05b1b058324b10a609d89d7833c1df2021-11-15T15:59:42ZQuorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T110.1128/mBio.01884-192150-7511https://doaj.org/article/6e05b1b058324b10a609d89d7833c1df2019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01884-19https://doaj.org/toc/2150-7511ABSTRACT Bacterial viruses, or bacteriophages, are highly abundant in the biosphere and have a major impact on microbial populations. Many examples of phage interactions with their hosts, including establishment of dormant lysogenic and active lytic states, have been characterized at the level of the individual cell. However, much less is known about the dependence of these interactions on host metabolism and signal exchange within bacterial communities. In this report, we describe a lysogenic state of the enterobacterial phage T1, previously known as a classical lytic phage, and characterize the underlying regulatory circuitry. We show that the transition from lysogeny to lysis depends on bacterial population density, perceived via interspecies autoinducer 2. Lysis is further controlled by the metabolic state of the cell, mediated by the cyclic-3′,5′-AMP (cAMP) receptor protein (CRP) of the host. We hypothesize that such combinations of cell density and metabolic sensing may be common in phage-host interactions. IMPORTANCE The dynamics of microbial communities are heavily shaped by bacterium-bacteriophage interactions. But despite the apparent importance of bacteriophages, our understanding of the mechanisms controlling phage dynamics in bacterial populations, and particularly of the differences between the decisions that are made in the dormant lysogenic and active lytic states, remains limited. In this report, we show that enterobacterial phage T1, previously described as a lytic phage, is able to undergo lysogeny. We further demonstrate that the lysogeny-to-lysis decision occurs in response to changes in the density of the bacterial population, mediated by interspecies quorum-sensing signal AI-2, and in the metabolic state of the cell, mediated by cAMP receptor protein. We hypothesize that this strategy enables the phage to maximize its chances of self-amplification and spreading in bacterial population upon induction of the lytic cycle and that it might be common in phage-host interactions.Leanid LaganenkaTimur SanderAlexander LagonenkoYu ChenHannes LinkVictor SourjikAmerican Society for MicrobiologyarticleEscherichia colibacteriophage lysiscyclic AMPquorum sensingMicrobiologyQR1-502ENmBio, Vol 10, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli bacteriophage lysis cyclic AMP quorum sensing Microbiology QR1-502
spellingShingle	Escherichia coli bacteriophage lysis cyclic AMP quorum sensing Microbiology QR1-502 Leanid Laganenka Timur Sander Alexander Lagonenko Yu Chen Hannes Link Victor Sourjik Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1
description	ABSTRACT Bacterial viruses, or bacteriophages, are highly abundant in the biosphere and have a major impact on microbial populations. Many examples of phage interactions with their hosts, including establishment of dormant lysogenic and active lytic states, have been characterized at the level of the individual cell. However, much less is known about the dependence of these interactions on host metabolism and signal exchange within bacterial communities. In this report, we describe a lysogenic state of the enterobacterial phage T1, previously known as a classical lytic phage, and characterize the underlying regulatory circuitry. We show that the transition from lysogeny to lysis depends on bacterial population density, perceived via interspecies autoinducer 2. Lysis is further controlled by the metabolic state of the cell, mediated by the cyclic-3′,5′-AMP (cAMP) receptor protein (CRP) of the host. We hypothesize that such combinations of cell density and metabolic sensing may be common in phage-host interactions. IMPORTANCE The dynamics of microbial communities are heavily shaped by bacterium-bacteriophage interactions. But despite the apparent importance of bacteriophages, our understanding of the mechanisms controlling phage dynamics in bacterial populations, and particularly of the differences between the decisions that are made in the dormant lysogenic and active lytic states, remains limited. In this report, we show that enterobacterial phage T1, previously described as a lytic phage, is able to undergo lysogeny. We further demonstrate that the lysogeny-to-lysis decision occurs in response to changes in the density of the bacterial population, mediated by interspecies quorum-sensing signal AI-2, and in the metabolic state of the cell, mediated by cAMP receptor protein. We hypothesize that this strategy enables the phage to maximize its chances of self-amplification and spreading in bacterial population upon induction of the lytic cycle and that it might be common in phage-host interactions.
format	article
author	Leanid Laganenka Timur Sander Alexander Lagonenko Yu Chen Hannes Link Victor Sourjik
author_facet	Leanid Laganenka Timur Sander Alexander Lagonenko Yu Chen Hannes Link Victor Sourjik
author_sort	Leanid Laganenka
title	Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1
title_short	Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1
title_full	Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1
title_fullStr	Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1
title_full_unstemmed	Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1
title_sort	quorum sensing and metabolic state of the host control lysogeny-lysis switch of bacteriophage t1
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/6e05b1b058324b10a609d89d7833c1df
work_keys_str_mv	AT leanidlaganenka quorumsensingandmetabolicstateofthehostcontrollysogenylysisswitchofbacteriophaget1 AT timursander quorumsensingandmetabolicstateofthehostcontrollysogenylysisswitchofbacteriophaget1 AT alexanderlagonenko quorumsensingandmetabolicstateofthehostcontrollysogenylysisswitchofbacteriophaget1 AT yuchen quorumsensingandmetabolicstateofthehostcontrollysogenylysisswitchofbacteriophaget1 AT hanneslink quorumsensingandmetabolicstateofthehostcontrollysogenylysisswitchofbacteriophaget1 AT victorsourjik quorumsensingandmetabolicstateofthehostcontrollysogenylysisswitchofbacteriophaget1
_version_	1718426973299015680

Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1

Ejemplares similares