Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells.

Trophoblast giant cells (TGCs), a mouse trophoblast subtype, have large amounts of cytoplasm and high ploidy levels via endocycles. The diverse functions and gene expression profiles of TGCs have been studied well, but their nuclear structures remain unknown. In this study, we focus on Lamin B1, a n...

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Autores principales:	Hiromu Morimoto, Misuzu Ueno, Hideyuki Tanabe, Tomohiro Kono, Hidehiko Ogawa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e079b277a444030bd2c7a0fe7a9cd33
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spelling	oai:doaj.org-article:6e079b277a444030bd2c7a0fe7a9cd332021-12-02T20:07:00ZProgesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells.1932-620310.1371/journal.pone.0254674https://doaj.org/article/6e079b277a444030bd2c7a0fe7a9cd332021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254674https://doaj.org/toc/1932-6203Trophoblast giant cells (TGCs), a mouse trophoblast subtype, have large amounts of cytoplasm and high ploidy levels via endocycles. The diverse functions and gene expression profiles of TGCs have been studied well, but their nuclear structures remain unknown. In this study, we focus on Lamin B1, a nuclear lamina, and clarify its expression dynamics, regulation and roles in TGC functions. TGCs that differentiated from trophoblast stem cells were used. From days 0 to 9 after differentiation, the number of TGCs gradually increased, but the amount of LMNB1 peaked at day 3 and then slightly decreased. An immunostaining experiment showed that LMNB1-depleted TGCs increased after day 6 of differentiation. These LMNB1-depleted TGCs diffused peripheral localization of the heterochromatin marker H3K9me2 in the nuclei. However, LMINB1-knock down was not affected TGCs specific gene expression. We found that the death of TGCs also increased after day 6 of differentiation. Moreover, Lamin B1 loss and the cell death in TGCs were protected by 10-6 M progesterone. Our results conclude that progesterone protects against Lamin B1 loss and prolongs the life and function of TGCs.Hiromu MorimotoMisuzu UenoHideyuki TanabeTomohiro KonoHidehiko OgawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254674 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiromu Morimoto Misuzu Ueno Hideyuki Tanabe Tomohiro Kono Hidehiko Ogawa Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells.
description	Trophoblast giant cells (TGCs), a mouse trophoblast subtype, have large amounts of cytoplasm and high ploidy levels via endocycles. The diverse functions and gene expression profiles of TGCs have been studied well, but their nuclear structures remain unknown. In this study, we focus on Lamin B1, a nuclear lamina, and clarify its expression dynamics, regulation and roles in TGC functions. TGCs that differentiated from trophoblast stem cells were used. From days 0 to 9 after differentiation, the number of TGCs gradually increased, but the amount of LMNB1 peaked at day 3 and then slightly decreased. An immunostaining experiment showed that LMNB1-depleted TGCs increased after day 6 of differentiation. These LMNB1-depleted TGCs diffused peripheral localization of the heterochromatin marker H3K9me2 in the nuclei. However, LMINB1-knock down was not affected TGCs specific gene expression. We found that the death of TGCs also increased after day 6 of differentiation. Moreover, Lamin B1 loss and the cell death in TGCs were protected by 10-6 M progesterone. Our results conclude that progesterone protects against Lamin B1 loss and prolongs the life and function of TGCs.
format	article
author	Hiromu Morimoto Misuzu Ueno Hideyuki Tanabe Tomohiro Kono Hidehiko Ogawa
author_facet	Hiromu Morimoto Misuzu Ueno Hideyuki Tanabe Tomohiro Kono Hidehiko Ogawa
author_sort	Hiromu Morimoto
title	Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells.
title_short	Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells.
title_full	Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells.
title_fullStr	Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells.
title_full_unstemmed	Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells.
title_sort	progesterone depletion results in lamin b1 loss and induction of cell death in mouse trophoblast giant cells.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6e079b277a444030bd2c7a0fe7a9cd33
work_keys_str_mv	AT hiromumorimoto progesteronedepletionresultsinlaminb1lossandinductionofcelldeathinmousetrophoblastgiantcells AT misuzuueno progesteronedepletionresultsinlaminb1lossandinductionofcelldeathinmousetrophoblastgiantcells AT hideyukitanabe progesteronedepletionresultsinlaminb1lossandinductionofcelldeathinmousetrophoblastgiantcells AT tomohirokono progesteronedepletionresultsinlaminb1lossandinductionofcelldeathinmousetrophoblastgiantcells AT hidehikoogawa progesteronedepletionresultsinlaminb1lossandinductionofcelldeathinmousetrophoblastgiantcells
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Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells.

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