Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.

Since the advent of highly pathogenic variants of avian influenza virus (HPAIV), the main focus of avian influenza research has been the characterization and detection of HPAIV hemagglutinin (HA) from H5 and H7 subtypes. However, due to the high mutation and reassortation rate of influenza viruses,...

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Autores principales:	Matthias Mueller, Sandra Renzullo, Roxann Brooks, Nicolas Ruggli, Martin A Hofmann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e0b0152afef41d7af7d05cf780b9d2b
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spelling	oai:doaj.org-article:6e0b0152afef41d7af7d05cf780b9d2b2021-11-25T06:26:04ZAntigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.1932-620310.1371/journal.pone.0009097https://doaj.org/article/6e0b0152afef41d7af7d05cf780b9d2b2010-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20140098/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Since the advent of highly pathogenic variants of avian influenza virus (HPAIV), the main focus of avian influenza research has been the characterization and detection of HPAIV hemagglutinin (HA) from H5 and H7 subtypes. However, due to the high mutation and reassortation rate of influenza viruses, in theory any influenza strain may acquire increased pathogenicity irrespective of its subtype. A comprehensive antigenic characterization of influenza viruses encompassing all 16 HA and 9 neuraminidase subtypes will provide information useful for the design of differential diagnostic tools, and possibly, vaccines. We have expressed recombinant HA proteins from 3 different influenza virus HA subtypes in the baculovirus system. These proteins were used to generate polyclonal rabbit antisera, which were subsequently employed in epitope scanning analysis using peptide libraries spanning the entire HA. Here, we report the identification and characterization of linear, HA subtype-specific as well as inter subtype-conserved epitopes along the HA proteins. Selected subtype-specific epitopes were shown to be suitable for the differentiation of anti-HA antibodies in an ELISA.Matthias MuellerSandra RenzulloRoxann BrooksNicolas RuggliMartin A HofmannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 2, p e9097 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthias Mueller Sandra Renzullo Roxann Brooks Nicolas Ruggli Martin A Hofmann Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.
description	Since the advent of highly pathogenic variants of avian influenza virus (HPAIV), the main focus of avian influenza research has been the characterization and detection of HPAIV hemagglutinin (HA) from H5 and H7 subtypes. However, due to the high mutation and reassortation rate of influenza viruses, in theory any influenza strain may acquire increased pathogenicity irrespective of its subtype. A comprehensive antigenic characterization of influenza viruses encompassing all 16 HA and 9 neuraminidase subtypes will provide information useful for the design of differential diagnostic tools, and possibly, vaccines. We have expressed recombinant HA proteins from 3 different influenza virus HA subtypes in the baculovirus system. These proteins were used to generate polyclonal rabbit antisera, which were subsequently employed in epitope scanning analysis using peptide libraries spanning the entire HA. Here, we report the identification and characterization of linear, HA subtype-specific as well as inter subtype-conserved epitopes along the HA proteins. Selected subtype-specific epitopes were shown to be suitable for the differentiation of anti-HA antibodies in an ELISA.
format	article
author	Matthias Mueller Sandra Renzullo Roxann Brooks Nicolas Ruggli Martin A Hofmann
author_facet	Matthias Mueller Sandra Renzullo Roxann Brooks Nicolas Ruggli Martin A Hofmann
author_sort	Matthias Mueller
title	Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.
title_short	Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.
title_full	Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.
title_fullStr	Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.
title_full_unstemmed	Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.
title_sort	antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/6e0b0152afef41d7af7d05cf780b9d2b
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Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes.

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