Intrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats

Nitric oxide (NO) has been shown to stimulate differentiation and increase the survival of ganglionic sympathetic neurons. The proportion of neuronal NOS-immunoreactive sympathetic preganglionic neurons is particularly high in newborn rats and decreases with maturation. However, the role of NO in th...

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Autores principales:	Ekaterina K. Selivanova, Anastasia A. Shvetsova, Anna A. Borzykh, Dina K. Gaynullina, Oxana O. Kiryukhina, Elena V. Lukoshkova, Viktoria M. Potekhina, Vladislav S. Kuzmin, Olga S. Tarasova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nitric oxide sympathetic innervation early postnatal development vasculature blood pressure Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/6e0d0c862ecb49b6b47429b202528a5b
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spelling	oai:doaj.org-article:6e0d0c862ecb49b6b47429b202528a5b2021-11-25T17:55:25ZIntrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats10.3390/ijms2222123271422-00671661-6596https://doaj.org/article/6e0d0c862ecb49b6b47429b202528a5b2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12327https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Nitric oxide (NO) has been shown to stimulate differentiation and increase the survival of ganglionic sympathetic neurons. The proportion of neuronal NOS-immunoreactive sympathetic preganglionic neurons is particularly high in newborn rats and decreases with maturation. However, the role of NO in the development of vascular sympathetic innervation has never been studied before. We tested the hypothesis that intrauterine NO deficiency weakened the development of vascular sympathetic innervation and thereby changed the contractility of peripheral arteries and blood pressure level in two-week-old offspring. Pregnant rats consumed NOS inhibitor L-NAME (250 mg/L in drinking water) from gestational day 10 until delivery. Pups in the L-NAME group had a reduced body weight and blood level of NO metabolites at 1–2 postnatal days. Saphenous arteries from two-week-old L-NAME offspring demonstrated a lower density of sympathetic innervation, a smaller inner diameter, reduced maximal active force and decreased α-actin/β-actin mRNA expression ratio compared to the controls. Importantly, pups in the L-NAME group exhibited decreased blood pressure levels before, but not after, ganglionic blockade with chlorisondamine. In conclusion, intrauterine L-NAME exposure is followed by the impaired development of the sympathetic nervous system in early postnatal life, which is accompanied by the structural and functional remodeling of arterial blood vessels.Ekaterina K. SelivanovaAnastasia A. ShvetsovaAnna A. BorzykhDina K. GaynullinaOxana O. KiryukhinaElena V. LukoshkovaViktoria M. PotekhinaVladislav S. KuzminOlga S. TarasovaMDPI AGarticlenitric oxidesympathetic innervationearly postnatal developmentvasculatureblood pressureBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12327, p 12327 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nitric oxide sympathetic innervation early postnatal development vasculature blood pressure Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	nitric oxide sympathetic innervation early postnatal development vasculature blood pressure Biology (General) QH301-705.5 Chemistry QD1-999 Ekaterina K. Selivanova Anastasia A. Shvetsova Anna A. Borzykh Dina K. Gaynullina Oxana O. Kiryukhina Elena V. Lukoshkova Viktoria M. Potekhina Vladislav S. Kuzmin Olga S. Tarasova Intrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats
description	Nitric oxide (NO) has been shown to stimulate differentiation and increase the survival of ganglionic sympathetic neurons. The proportion of neuronal NOS-immunoreactive sympathetic preganglionic neurons is particularly high in newborn rats and decreases with maturation. However, the role of NO in the development of vascular sympathetic innervation has never been studied before. We tested the hypothesis that intrauterine NO deficiency weakened the development of vascular sympathetic innervation and thereby changed the contractility of peripheral arteries and blood pressure level in two-week-old offspring. Pregnant rats consumed NOS inhibitor L-NAME (250 mg/L in drinking water) from gestational day 10 until delivery. Pups in the L-NAME group had a reduced body weight and blood level of NO metabolites at 1–2 postnatal days. Saphenous arteries from two-week-old L-NAME offspring demonstrated a lower density of sympathetic innervation, a smaller inner diameter, reduced maximal active force and decreased α-actin/β-actin mRNA expression ratio compared to the controls. Importantly, pups in the L-NAME group exhibited decreased blood pressure levels before, but not after, ganglionic blockade with chlorisondamine. In conclusion, intrauterine L-NAME exposure is followed by the impaired development of the sympathetic nervous system in early postnatal life, which is accompanied by the structural and functional remodeling of arterial blood vessels.
format	article
author	Ekaterina K. Selivanova Anastasia A. Shvetsova Anna A. Borzykh Dina K. Gaynullina Oxana O. Kiryukhina Elena V. Lukoshkova Viktoria M. Potekhina Vladislav S. Kuzmin Olga S. Tarasova
author_facet	Ekaterina K. Selivanova Anastasia A. Shvetsova Anna A. Borzykh Dina K. Gaynullina Oxana O. Kiryukhina Elena V. Lukoshkova Viktoria M. Potekhina Vladislav S. Kuzmin Olga S. Tarasova
author_sort	Ekaterina K. Selivanova
title	Intrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats
title_short	Intrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats
title_full	Intrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats
title_fullStr	Intrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats
title_full_unstemmed	Intrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats
title_sort	intrauterine l-name exposure weakens the development of sympathetic innervation and induces the remodeling of arterial vessels in two-week-old rats
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6e0d0c862ecb49b6b47429b202528a5b
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Intrauterine L-NAME Exposure Weakens the Development of Sympathetic Innervation and Induces the Remodeling of Arterial Vessels in Two-Week-Old Rats

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