A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications

Abstract The effectiveness of nanoparticles (NP) in nanomedicine depends on their ability to extravasate from vasculature towards the target tissue. This is determined by their permeability across the endothelial barrier. Unfortunately, a quantitative study of the diffusion permeability coefficients...

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Autores principales:	Yan Teck Ho, Giulia Adriani, Sebastian Beyer, Phan-Thien Nhan, Roger D. Kamm, James Chen Yong Kah
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e0f5d2ae4834e9e8112c4f27b90b1d4
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spelling	oai:doaj.org-article:6e0f5d2ae4834e9e8112c4f27b90b1d42021-12-02T16:06:46ZA Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications10.1038/s41598-017-00750-32045-2322https://doaj.org/article/6e0f5d2ae4834e9e8112c4f27b90b1d42017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00750-3https://doaj.org/toc/2045-2322Abstract The effectiveness of nanoparticles (NP) in nanomedicine depends on their ability to extravasate from vasculature towards the target tissue. This is determined by their permeability across the endothelial barrier. Unfortunately, a quantitative study of the diffusion permeability coefficients (Pd) of NPs is difficult with in vivo models. Here, we utilize a relevant model of vascular-tissue interface with tunable endothelial permeability in vitro based on microfluidics. Human umbilical vein endothelial cells (HUVECs) grown in microfluidic devices were treated with Angiopoietin 1 and cyclic adenosine monophosphate (cAMP) to vary the Pd of the HUVECs monolayer towards fluorescent polystyrene NPs (pNPs) of different sizes, which was determined from image analysis of their fluorescence intensity when diffusing across the monolayer. Using 70 kDa dextran as a probe, untreated HUVECs yielded a Pd that approximated tumor vasculature while HUVECs treated with 25 μg/mL cAMP had Pd that approximated healthy vasculature in vivo. As the size of pNPs increased, its Pd decreased in tumor vasculature, but remained largely unchanged in healthy vasculature, demonstrating a trend similar to tumor selectivity for smaller NPs. This microfluidic model of vascular-tissue interface can be used in any laboratory to perform quantitative assessment of the tumor selectivity of nanomedicine-based systems.Yan Teck HoGiulia AdrianiSebastian BeyerPhan-Thien NhanRoger D. KammJames Chen Yong KahNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Teck Ho Giulia Adriani Sebastian Beyer Phan-Thien Nhan Roger D. Kamm James Chen Yong Kah A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
description	Abstract The effectiveness of nanoparticles (NP) in nanomedicine depends on their ability to extravasate from vasculature towards the target tissue. This is determined by their permeability across the endothelial barrier. Unfortunately, a quantitative study of the diffusion permeability coefficients (Pd) of NPs is difficult with in vivo models. Here, we utilize a relevant model of vascular-tissue interface with tunable endothelial permeability in vitro based on microfluidics. Human umbilical vein endothelial cells (HUVECs) grown in microfluidic devices were treated with Angiopoietin 1 and cyclic adenosine monophosphate (cAMP) to vary the Pd of the HUVECs monolayer towards fluorescent polystyrene NPs (pNPs) of different sizes, which was determined from image analysis of their fluorescence intensity when diffusing across the monolayer. Using 70 kDa dextran as a probe, untreated HUVECs yielded a Pd that approximated tumor vasculature while HUVECs treated with 25 μg/mL cAMP had Pd that approximated healthy vasculature in vivo. As the size of pNPs increased, its Pd decreased in tumor vasculature, but remained largely unchanged in healthy vasculature, demonstrating a trend similar to tumor selectivity for smaller NPs. This microfluidic model of vascular-tissue interface can be used in any laboratory to perform quantitative assessment of the tumor selectivity of nanomedicine-based systems.
format	article
author	Yan Teck Ho Giulia Adriani Sebastian Beyer Phan-Thien Nhan Roger D. Kamm James Chen Yong Kah
author_facet	Yan Teck Ho Giulia Adriani Sebastian Beyer Phan-Thien Nhan Roger D. Kamm James Chen Yong Kah
author_sort	Yan Teck Ho
title	A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
title_short	A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
title_full	A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
title_fullStr	A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
title_full_unstemmed	A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
title_sort	facile method to probe the vascular permeability of nanoparticles in nanomedicine applications
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6e0f5d2ae4834e9e8112c4f27b90b1d4
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A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications

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