Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection

This article describes the initial study on the simultaneous determination of multiclass antibiotic residues in imported and local frozen poultry specimens, including turkey gizzard and muscle tissues, and chicken muscle tissues, commonly consumed in Ogun State, Nigeria. Minced tissues were treated...

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Autores principales:	Abdulrasaq O. Oyedeji, Titus A.M. Msagati, Akan B. Williams, Nsikak U. Benson
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Antibiotic residues Food contamination Solid-phase extraction High-performance liquid chromatography Poultry tissues Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/6e1ea0cdba1e4939bdc907da17a2771a
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spelling	oai:doaj.org-article:6e1ea0cdba1e4939bdc907da17a2771a2021-12-04T04:35:24ZDetection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection2405-844010.1016/j.heliyon.2021.e08469https://doaj.org/article/6e1ea0cdba1e4939bdc907da17a2771a2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S240584402102572Xhttps://doaj.org/toc/2405-8440This article describes the initial study on the simultaneous determination of multiclass antibiotic residues in imported and local frozen poultry specimens, including turkey gizzard and muscle tissues, and chicken muscle tissues, commonly consumed in Ogun State, Nigeria. Minced tissues were treated with phosphate buffer adjusted to pH 7 that was cleaned using C18 SPE-column (Supelclean™) cartridge. For the determination of six antibiotic residues including fluoroquinolones, sulfonamides, and macrolides, a solid-phase extraction method was used, followed by extract analysis using high-performance liquid chromatography–diode array detection (HPLC–DAD). The coefficient of determination (R2) for the external standards for all the analytes ranged between 0.963 and 0.999. The limit of detection (LOD) and quantification (LOQ) ranged between 5.37 – 55.4 μg/kg, and 17.9–185 μg/kg, respectively. Enrofloxacin, sulfadimethoxine, sulfamerazine, and tylosin showed high concentration levels in the frozen poultry beyond acceptable maximum residue limits (MRLs). The six drugs considered in this study were present at higher concentrations in domestic chicken tissues than the permissible level. This suggests that farmers do not observe the cessation period before poultry birds previously treated with antibiotics are sold to consumers thus exposing them to potentially hazardous antibiotic residues.Abdulrasaq O. OyedejiTitus A.M. MsagatiAkan B. WilliamsNsikak U. BensonElsevierarticleAntibiotic residuesFood contaminationSolid-phase extractionHigh-performance liquid chromatographyPoultry tissuesScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 12, Pp e08469- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antibiotic residues Food contamination Solid-phase extraction High-performance liquid chromatography Poultry tissues Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Antibiotic residues Food contamination Solid-phase extraction High-performance liquid chromatography Poultry tissues Science (General) Q1-390 Social sciences (General) H1-99 Abdulrasaq O. Oyedeji Titus A.M. Msagati Akan B. Williams Nsikak U. Benson Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection
description	This article describes the initial study on the simultaneous determination of multiclass antibiotic residues in imported and local frozen poultry specimens, including turkey gizzard and muscle tissues, and chicken muscle tissues, commonly consumed in Ogun State, Nigeria. Minced tissues were treated with phosphate buffer adjusted to pH 7 that was cleaned using C18 SPE-column (Supelclean™) cartridge. For the determination of six antibiotic residues including fluoroquinolones, sulfonamides, and macrolides, a solid-phase extraction method was used, followed by extract analysis using high-performance liquid chromatography–diode array detection (HPLC–DAD). The coefficient of determination (R2) for the external standards for all the analytes ranged between 0.963 and 0.999. The limit of detection (LOD) and quantification (LOQ) ranged between 5.37 – 55.4 μg/kg, and 17.9–185 μg/kg, respectively. Enrofloxacin, sulfadimethoxine, sulfamerazine, and tylosin showed high concentration levels in the frozen poultry beyond acceptable maximum residue limits (MRLs). The six drugs considered in this study were present at higher concentrations in domestic chicken tissues than the permissible level. This suggests that farmers do not observe the cessation period before poultry birds previously treated with antibiotics are sold to consumers thus exposing them to potentially hazardous antibiotic residues.
format	article
author	Abdulrasaq O. Oyedeji Titus A.M. Msagati Akan B. Williams Nsikak U. Benson
author_facet	Abdulrasaq O. Oyedeji Titus A.M. Msagati Akan B. Williams Nsikak U. Benson
author_sort	Abdulrasaq O. Oyedeji
title	Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection
title_short	Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection
title_full	Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection
title_fullStr	Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection
title_full_unstemmed	Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection
title_sort	detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/6e1ea0cdba1e4939bdc907da17a2771a
work_keys_str_mv	AT abdulrasaqooyedeji detectionandquantificationofmulticlassantibioticresiduesinpoultryproductsusingsolidphaseextractionandhighperformanceliquidchromatographywithdiodearraydetection AT titusammsagati detectionandquantificationofmulticlassantibioticresiduesinpoultryproductsusingsolidphaseextractionandhighperformanceliquidchromatographywithdiodearraydetection AT akanbwilliams detectionandquantificationofmulticlassantibioticresiduesinpoultryproductsusingsolidphaseextractionandhighperformanceliquidchromatographywithdiodearraydetection AT nsikakubenson detectionandquantificationofmulticlassantibioticresiduesinpoultryproductsusingsolidphaseextractionandhighperformanceliquidchromatographywithdiodearraydetection
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Detection and quantification of multiclass antibiotic residues in poultry products using solid-phase extraction and high-performance liquid chromatography with diode array detection

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