The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.

RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase...

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Autores principales:	Yang Qiu, Zhaowei Wang, Yongxiang Liu, Yajuan Han, Meng Miao, Nan Qi, Jie Yang, Hongjie Xia, Xiaofeng Li, Cheng-Feng Qin, Yuanyang Hu, Xi Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e1ee54e0c5c45ef823173106c5f2041
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spelling	oai:doaj.org-article:6e1ee54e0c5c45ef823173106c5f20412021-11-18T08:31:07ZThe self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.1932-620310.1371/journal.pone.0089628https://doaj.org/article/6e1ee54e0c5c45ef823173106c5f20412014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24586921/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase required for viral RNA replication. Here, we studied the direct effects of mitochondrial membrane lipids (MMLs) on WhNV protein A activity in vitro. Our investigations revealed the self-interaction of WhNV protein A is accomplished via two different patterns (i.e., homotypic and heterotypic self-interactions via different interfaces). MMLs stimulated the protein A self-interaction, and this stimulation exhibited selectivity for specific phospholipids. Moreover, we found that specific phospholipids differently favor the two self-interaction patterns. Furthermore, manipulating specific phospholipid metabolism affected protein A self-interaction and the activity of protein A to replicate RNA in cells. Taken together, our findings reveal the direct effects of membrane lipids on a nodaviral RNA replicase.Yang QiuZhaowei WangYongxiang LiuYajuan HanMeng MiaoNan QiJie YangHongjie XiaXiaofeng LiCheng-Feng QinYuanyang HuXi ZhouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e89628 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Qiu Zhaowei Wang Yongxiang Liu Yajuan Han Meng Miao Nan Qi Jie Yang Hongjie Xia Xiaofeng Li Cheng-Feng Qin Yuanyang Hu Xi Zhou The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.
description	RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase required for viral RNA replication. Here, we studied the direct effects of mitochondrial membrane lipids (MMLs) on WhNV protein A activity in vitro. Our investigations revealed the self-interaction of WhNV protein A is accomplished via two different patterns (i.e., homotypic and heterotypic self-interactions via different interfaces). MMLs stimulated the protein A self-interaction, and this stimulation exhibited selectivity for specific phospholipids. Moreover, we found that specific phospholipids differently favor the two self-interaction patterns. Furthermore, manipulating specific phospholipid metabolism affected protein A self-interaction and the activity of protein A to replicate RNA in cells. Taken together, our findings reveal the direct effects of membrane lipids on a nodaviral RNA replicase.
format	article
author	Yang Qiu Zhaowei Wang Yongxiang Liu Yajuan Han Meng Miao Nan Qi Jie Yang Hongjie Xia Xiaofeng Li Cheng-Feng Qin Yuanyang Hu Xi Zhou
author_facet	Yang Qiu Zhaowei Wang Yongxiang Liu Yajuan Han Meng Miao Nan Qi Jie Yang Hongjie Xia Xiaofeng Li Cheng-Feng Qin Yuanyang Hu Xi Zhou
author_sort	Yang Qiu
title	The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.
title_short	The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.
title_full	The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.
title_fullStr	The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.
title_full_unstemmed	The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.
title_sort	self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/6e1ee54e0c5c45ef823173106c5f2041
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The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.

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