Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription

Abstract As a desirable agricultural trait, multilocular trait of rapeseed (Brassica rapa; Brassica napus; Brassica juncea), always represents higher yield per plant compared with bilocular plants. We previously isolated a trilocular gene locus, Bjmc1, and identified a set of molecular markers linke...

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Autores principales:	Ping Xu, Shiqin Cao, Kaining Hu, Xiaohua Wang, Wei Huang, Gang Wang, Zewen Lv, Zhongsong Liu, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Tingdong Fu, Jinxiong Shen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e20cb7c6ec64b8fa3c987b35681be54
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spelling	oai:doaj.org-article:6e20cb7c6ec64b8fa3c987b35681be542021-12-02T12:32:13ZTrilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription10.1038/s41598-017-03755-02045-2322https://doaj.org/article/6e20cb7c6ec64b8fa3c987b35681be542017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03755-0https://doaj.org/toc/2045-2322Abstract As a desirable agricultural trait, multilocular trait of rapeseed (Brassica rapa; Brassica napus; Brassica juncea), always represents higher yield per plant compared with bilocular plants. We previously isolated a trilocular gene locus, Bjmc1, and identified a set of molecular markers linked to the trilocular gene. With a map-based cloning, we identified that the BjMc1 was located in B genome of Brassica juncea, and it was a CLAVATA1 (CLV1) gene homologue. The insertion of a copia-LTR retrotransposable element 1 (RTE1) into the coding region of BjMc1 interrupted its transcription in rapeseed, leading to the trilocular phenotype. Phylogenetic analysis showed that Mc1 genes were conserved and widespread in land plants. Two amino acid sites had undergone positive selection in the ancestor of Mc1 genes, and then purifying selection was the dominant force after the divergence of dicots and monocots from their common ancestor in the evolutionary process, indicating that Mc1 genes are conserved in modern land plants. Our results provided new insights in molecular regulatory mechanism of multilocularity in rapeseed, and better understanding of molecular mechanism in crop yield improvement.Ping XuShiqin CaoKaining HuXiaohua WangWei HuangGang WangZewen LvZhongsong LiuJing WenBin YiChaozhi MaJinxing TuTingdong FuJinxiong ShenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ping Xu Shiqin Cao Kaining Hu Xiaohua Wang Wei Huang Gang Wang Zewen Lv Zhongsong Liu Jing Wen Bin Yi Chaozhi Ma Jinxing Tu Tingdong Fu Jinxiong Shen Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription
description	Abstract As a desirable agricultural trait, multilocular trait of rapeseed (Brassica rapa; Brassica napus; Brassica juncea), always represents higher yield per plant compared with bilocular plants. We previously isolated a trilocular gene locus, Bjmc1, and identified a set of molecular markers linked to the trilocular gene. With a map-based cloning, we identified that the BjMc1 was located in B genome of Brassica juncea, and it was a CLAVATA1 (CLV1) gene homologue. The insertion of a copia-LTR retrotransposable element 1 (RTE1) into the coding region of BjMc1 interrupted its transcription in rapeseed, leading to the trilocular phenotype. Phylogenetic analysis showed that Mc1 genes were conserved and widespread in land plants. Two amino acid sites had undergone positive selection in the ancestor of Mc1 genes, and then purifying selection was the dominant force after the divergence of dicots and monocots from their common ancestor in the evolutionary process, indicating that Mc1 genes are conserved in modern land plants. Our results provided new insights in molecular regulatory mechanism of multilocularity in rapeseed, and better understanding of molecular mechanism in crop yield improvement.
format	article
author	Ping Xu Shiqin Cao Kaining Hu Xiaohua Wang Wei Huang Gang Wang Zewen Lv Zhongsong Liu Jing Wen Bin Yi Chaozhi Ma Jinxing Tu Tingdong Fu Jinxiong Shen
author_facet	Ping Xu Shiqin Cao Kaining Hu Xiaohua Wang Wei Huang Gang Wang Zewen Lv Zhongsong Liu Jing Wen Bin Yi Chaozhi Ma Jinxing Tu Tingdong Fu Jinxiong Shen
author_sort	Ping Xu
title	Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription
title_short	Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription
title_full	Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription
title_fullStr	Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription
title_full_unstemmed	Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription
title_sort	trilocular phenotype in brassica juncea l. resulted from interruption of clavata1 gene homologue (bjmc1) transcription
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6e20cb7c6ec64b8fa3c987b35681be54
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Trilocular phenotype in Brassica juncea L. resulted from interruption of CLAVATA1 gene homologue (BjMc1) transcription

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