Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes

Abstract Mycobacterium bovis is the causative agent of bovine tuberculosis (bTB) in wildlife. Confirmation of M. bovis infection relies on mycobacterial culture, which is time-consuming. Collection and transportation of infectious material also pose a human health risk. PrimeStore Molecular Transpor...

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Autores principales:	Charlene Clarke, Katrin Smith, Samantha J. Goldswain, Christopher Helm, David V. Cooper, Tanya J. Kerr, Léanie Kleynhans, Paul D. van Helden, Robin M. Warren, Michele A. Miller, Wynand J. Goosen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e278a5b15124511bbb3bddac57174b5
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spelling	oai:doaj.org-article:6e278a5b15124511bbb3bddac57174b52021-12-02T14:25:21ZNovel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes10.1038/s41598-021-86682-52045-2322https://doaj.org/article/6e278a5b15124511bbb3bddac57174b52021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86682-5https://doaj.org/toc/2045-2322Abstract Mycobacterium bovis is the causative agent of bovine tuberculosis (bTB) in wildlife. Confirmation of M. bovis infection relies on mycobacterial culture, which is time-consuming. Collection and transportation of infectious material also pose a human health risk. PrimeStore Molecular Transport Medium (MTM) has been shown to effectively inactivate infectious organisms, making it a safe method for handling infectious samples. This study investigated an in-field sampling technique for rapid, safe detection of M. bovis in buffalo tissues. Potentially infected tissues from bTB test-positive buffaloes were swabbed at post-mortem examination and stored in PrimeStore MTM at ambient temperature until Xpert MTB/RIF Ultra testing was performed. Additionally, tissue samples were frozen and transported before homogenisation for culture and Ultra testing. Oral swabs were collected from M. bovis-unexposed buffaloes as a negative control cohort. Mycobacterium tuberculosis complex (MTBC) DNA was detected by Ultra in 13/16 tissue swabs and 9/16 matched tissue homogenates from culture-confirmed M. bovis-positive buffalo tissues. MTBC DNA was not detected in swabs from M. bovis-unexposed animals, showing the potentially high specificity of Ultra with PrimeStore swabs. PrimeStore MTM sample processing, in combination with the Ultra assay, has the potential to provide a safe, rapid post-mortem screening test for M. bovis in buffaloes.Charlene ClarkeKatrin SmithSamantha J. GoldswainChristopher HelmDavid V. CooperTanya J. KerrLéanie KleynhansPaul D. van HeldenRobin M. WarrenMichele A. MillerWynand J. GoosenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charlene Clarke Katrin Smith Samantha J. Goldswain Christopher Helm David V. Cooper Tanya J. Kerr Léanie Kleynhans Paul D. van Helden Robin M. Warren Michele A. Miller Wynand J. Goosen Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes
description	Abstract Mycobacterium bovis is the causative agent of bovine tuberculosis (bTB) in wildlife. Confirmation of M. bovis infection relies on mycobacterial culture, which is time-consuming. Collection and transportation of infectious material also pose a human health risk. PrimeStore Molecular Transport Medium (MTM) has been shown to effectively inactivate infectious organisms, making it a safe method for handling infectious samples. This study investigated an in-field sampling technique for rapid, safe detection of M. bovis in buffalo tissues. Potentially infected tissues from bTB test-positive buffaloes were swabbed at post-mortem examination and stored in PrimeStore MTM at ambient temperature until Xpert MTB/RIF Ultra testing was performed. Additionally, tissue samples were frozen and transported before homogenisation for culture and Ultra testing. Oral swabs were collected from M. bovis-unexposed buffaloes as a negative control cohort. Mycobacterium tuberculosis complex (MTBC) DNA was detected by Ultra in 13/16 tissue swabs and 9/16 matched tissue homogenates from culture-confirmed M. bovis-positive buffalo tissues. MTBC DNA was not detected in swabs from M. bovis-unexposed animals, showing the potentially high specificity of Ultra with PrimeStore swabs. PrimeStore MTM sample processing, in combination with the Ultra assay, has the potential to provide a safe, rapid post-mortem screening test for M. bovis in buffaloes.
format	article
author	Charlene Clarke Katrin Smith Samantha J. Goldswain Christopher Helm David V. Cooper Tanya J. Kerr Léanie Kleynhans Paul D. van Helden Robin M. Warren Michele A. Miller Wynand J. Goosen
author_facet	Charlene Clarke Katrin Smith Samantha J. Goldswain Christopher Helm David V. Cooper Tanya J. Kerr Léanie Kleynhans Paul D. van Helden Robin M. Warren Michele A. Miller Wynand J. Goosen
author_sort	Charlene Clarke
title	Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes
title_short	Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes
title_full	Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes
title_fullStr	Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes
title_full_unstemmed	Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes
title_sort	novel molecular transport medium used in combination with xpert mtb/rif ultra provides rapid detection of mycobacterium bovis in african buffaloes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6e278a5b15124511bbb3bddac57174b5
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Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes

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