MAPK/ERK and JNK pathways regulate lipid synthesis and cell growth of Chlamydomonas reinhardtii under osmotic stress, respectively

Abstract Microalgae have great potential for the production of biofuels due to the ability of the organism to accumulate large quantities of storage lipids under stress conditions. Mitogen activated protein kinase (MAPK) signaling cascades are widely recognized for their role in stress response sign...

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Autores principales:	Ahreum Yang, William I. Suh, Nam Kyu Kang, Bongsoo Lee, Yong Keun Chang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Reinhardtii Environmental Osmotic Stress Lipid Production Extracellular Signal-regulated Kinase Neutral Lipid Accumulation Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e2861b80a0b46a0bf34217b610792f5
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spelling	oai:doaj.org-article:6e2861b80a0b46a0bf34217b610792f52021-12-02T15:08:11ZMAPK/ERK and JNK pathways regulate lipid synthesis and cell growth of Chlamydomonas reinhardtii under osmotic stress, respectively10.1038/s41598-018-32216-52045-2322https://doaj.org/article/6e2861b80a0b46a0bf34217b610792f52018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32216-5https://doaj.org/toc/2045-2322Abstract Microalgae have great potential for the production of biofuels due to the ability of the organism to accumulate large quantities of storage lipids under stress conditions. Mitogen activated protein kinase (MAPK) signaling cascades are widely recognized for their role in stress response signal transduction in eukaryotes. To assess the correlation between MAPK activation and lipid productivity, Chlamydomonas reinhardtii was studied under various concentrations of NaCl. The results demonstrated that C. reinhardtii exhibits elevated levels of extracellular-signal regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) activities after undergoing osmotic stress, as well as an increase in cellular lipid content. To establish a more direct causal link between both kinases and lipid productivity, C. reinhardtii was subjected to biochemically induced regulation of ERK and JNK pathways. Activating the MEK-ERK pathway via C6 ceramide treatment increased ERK activation and lipid production simultaneously, while PD98059 mediated inhibition of the pathway yielded opposite results. Interestingly, suppression of the JNK pathway with SP600125 resulted in a substantial decrease in cell viability under osmotic stress. These results suggest that ERK and JNK MAP kinases have important roles in microalgal lipid accumulation and cell growth under osmotic stress, respectively.Ahreum YangWilliam I. SuhNam Kyu KangBongsoo LeeYong Keun ChangNature PortfolioarticleReinhardtiiEnvironmental Osmotic StressLipid ProductionExtracellular Signal-regulated KinaseNeutral Lipid AccumulationMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Reinhardtii Environmental Osmotic Stress Lipid Production Extracellular Signal-regulated Kinase Neutral Lipid Accumulation Medicine R Science Q
spellingShingle	Reinhardtii Environmental Osmotic Stress Lipid Production Extracellular Signal-regulated Kinase Neutral Lipid Accumulation Medicine R Science Q Ahreum Yang William I. Suh Nam Kyu Kang Bongsoo Lee Yong Keun Chang MAPK/ERK and JNK pathways regulate lipid synthesis and cell growth of Chlamydomonas reinhardtii under osmotic stress, respectively
description	Abstract Microalgae have great potential for the production of biofuels due to the ability of the organism to accumulate large quantities of storage lipids under stress conditions. Mitogen activated protein kinase (MAPK) signaling cascades are widely recognized for their role in stress response signal transduction in eukaryotes. To assess the correlation between MAPK activation and lipid productivity, Chlamydomonas reinhardtii was studied under various concentrations of NaCl. The results demonstrated that C. reinhardtii exhibits elevated levels of extracellular-signal regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) activities after undergoing osmotic stress, as well as an increase in cellular lipid content. To establish a more direct causal link between both kinases and lipid productivity, C. reinhardtii was subjected to biochemically induced regulation of ERK and JNK pathways. Activating the MEK-ERK pathway via C6 ceramide treatment increased ERK activation and lipid production simultaneously, while PD98059 mediated inhibition of the pathway yielded opposite results. Interestingly, suppression of the JNK pathway with SP600125 resulted in a substantial decrease in cell viability under osmotic stress. These results suggest that ERK and JNK MAP kinases have important roles in microalgal lipid accumulation and cell growth under osmotic stress, respectively.
format	article
author	Ahreum Yang William I. Suh Nam Kyu Kang Bongsoo Lee Yong Keun Chang
author_facet	Ahreum Yang William I. Suh Nam Kyu Kang Bongsoo Lee Yong Keun Chang
author_sort	Ahreum Yang
title	MAPK/ERK and JNK pathways regulate lipid synthesis and cell growth of Chlamydomonas reinhardtii under osmotic stress, respectively
title_short	MAPK/ERK and JNK pathways regulate lipid synthesis and cell growth of Chlamydomonas reinhardtii under osmotic stress, respectively
title_full	MAPK/ERK and JNK pathways regulate lipid synthesis and cell growth of Chlamydomonas reinhardtii under osmotic stress, respectively
title_fullStr	MAPK/ERK and JNK pathways regulate lipid synthesis and cell growth of Chlamydomonas reinhardtii under osmotic stress, respectively
title_full_unstemmed	MAPK/ERK and JNK pathways regulate lipid synthesis and cell growth of Chlamydomonas reinhardtii under osmotic stress, respectively
title_sort	mapk/erk and jnk pathways regulate lipid synthesis and cell growth of chlamydomonas reinhardtii under osmotic stress, respectively
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6e2861b80a0b46a0bf34217b610792f5
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MAPK/ERK and JNK pathways regulate lipid synthesis and cell growth of Chlamydomonas reinhardtii under osmotic stress, respectively

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