Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing

Efficient deconvolution of antibody targets is needed for phenotype-based discovery. Here, the authors investigate a deconvolution approach based on pooled CRISPR Cas9 to achieve 97% deconvolution success rate.

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Auteurs principaux:	Jenny Mattsson, Ludvig Ekdahl, Fredrik Junghus, Ram Ajore, Eva Erlandsson, Abhishek Niroula, Maroulio Pertesi, Björn Frendéus, Ingrid Teige, Björn Nilsson
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/6e2a5135b9de48a8b79f53b16495e80f
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spelling	oai:doaj.org-article:6e2a5135b9de48a8b79f53b16495e80f2021-12-02T15:52:44ZAccelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing10.1038/s41467-021-21518-42041-1723https://doaj.org/article/6e2a5135b9de48a8b79f53b16495e80f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21518-4https://doaj.org/toc/2041-1723Efficient deconvolution of antibody targets is needed for phenotype-based discovery. Here, the authors investigate a deconvolution approach based on pooled CRISPR Cas9 to achieve 97% deconvolution success rate.Jenny MattssonLudvig EkdahlFredrik JunghusRam AjoreEva ErlandssonAbhishek NiroulaMaroulio PertesiBjörn FrendéusIngrid TeigeBjörn NilssonNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jenny Mattsson Ludvig Ekdahl Fredrik Junghus Ram Ajore Eva Erlandsson Abhishek Niroula Maroulio Pertesi Björn Frendéus Ingrid Teige Björn Nilsson Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing
description	Efficient deconvolution of antibody targets is needed for phenotype-based discovery. Here, the authors investigate a deconvolution approach based on pooled CRISPR Cas9 to achieve 97% deconvolution success rate.
format	article
author	Jenny Mattsson Ludvig Ekdahl Fredrik Junghus Ram Ajore Eva Erlandsson Abhishek Niroula Maroulio Pertesi Björn Frendéus Ingrid Teige Björn Nilsson
author_facet	Jenny Mattsson Ludvig Ekdahl Fredrik Junghus Ram Ajore Eva Erlandsson Abhishek Niroula Maroulio Pertesi Björn Frendéus Ingrid Teige Björn Nilsson
author_sort	Jenny Mattsson
title	Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing
title_short	Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing
title_full	Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing
title_fullStr	Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing
title_full_unstemmed	Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing
title_sort	accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6e2a5135b9de48a8b79f53b16495e80f
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Accelerating target deconvolution for therapeutic antibody candidates using highly parallelized genome editing

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