Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods

Abstract. Prihastanti E, Hastuti ED, Suedy SWA. 2020. Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods. Biodiversitas 21: 5845-5849. Several efforts have been made to preserve Citrus reti...

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Autores principales:	Erma Prihastanti, ENDAH D. HASTUTI, SRI W.A. SUEDY
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	citrus reticulata var. tawangmangu, citrus reticulata var. garut, callus growth, stem explants Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6e373ee0800944e1908ed4734b7ff312
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spelling	oai:doaj.org-article:6e373ee0800944e1908ed4734b7ff3122021-11-22T00:52:00ZShort Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods1412-033X2085-472210.13057/biodiv/d211248https://doaj.org/article/6e373ee0800944e1908ed4734b7ff3122020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6013https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Prihastanti E, Hastuti ED, Suedy SWA. 2020. Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods. Biodiversitas 21: 5845-5849. Several efforts have been made to preserve Citrus reticulata var. Tawangmangu and Citrus reticulata var. Garut as indigenous Indonesian mandarin cultivars, including in vitro tissue culture methods. This study aimed to determine growth differences of the stem explants of C. reticulata var. Tawangmangu and C. reticulata var. Garut,which planted on the same Murashige and Skoog (MS) media. The treatment groups were derived from different explants, grown in 4 separate culture bottles for 35 days at 25°C. The observed parameters included the percentage of explants indicating callus development browned-colored explants, and the contaminated explants. Among C. reticulata var. Tawangmangu explants, 23.53% indicated callus development, 29.42% were browned-colored explants, and 0% indicated contamination. In contrast, among the C. reticulata var. Garut explants, 0% indicated callus development, 7.14% brown-colored, and 7.14% indicated contamination. The stems explants from C. reticulata var. Tawangmangu showed a tendency to develop calluses, but the explants of C. reticulata var. Garut was able to support the growth of shoots. C. reticulata var. Tawangmangu and Garut mandarin stem explants showed differences of shoot growth because physiological conditions varied according to the variety.Erma PrihastantiENDAH D. HASTUTISRI W.A. SUEDYMBI & UNS Soloarticlecitrus reticulata var. tawangmangu, citrus reticulata var. garut, callus growth, stem explantsBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	citrus reticulata var. tawangmangu, citrus reticulata var. garut, callus growth, stem explants Biology (General) QH301-705.5
spellingShingle	citrus reticulata var. tawangmangu, citrus reticulata var. garut, callus growth, stem explants Biology (General) QH301-705.5 Erma Prihastanti ENDAH D. HASTUTI SRI W.A. SUEDY Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods
description	Abstract. Prihastanti E, Hastuti ED, Suedy SWA. 2020. Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods. Biodiversitas 21: 5845-5849. Several efforts have been made to preserve Citrus reticulata var. Tawangmangu and Citrus reticulata var. Garut as indigenous Indonesian mandarin cultivars, including in vitro tissue culture methods. This study aimed to determine growth differences of the stem explants of C. reticulata var. Tawangmangu and C. reticulata var. Garut,which planted on the same Murashige and Skoog (MS) media. The treatment groups were derived from different explants, grown in 4 separate culture bottles for 35 days at 25°C. The observed parameters included the percentage of explants indicating callus development browned-colored explants, and the contaminated explants. Among C. reticulata var. Tawangmangu explants, 23.53% indicated callus development, 29.42% were browned-colored explants, and 0% indicated contamination. In contrast, among the C. reticulata var. Garut explants, 0% indicated callus development, 7.14% brown-colored, and 7.14% indicated contamination. The stems explants from C. reticulata var. Tawangmangu showed a tendency to develop calluses, but the explants of C. reticulata var. Garut was able to support the growth of shoots. C. reticulata var. Tawangmangu and Garut mandarin stem explants showed differences of shoot growth because physiological conditions varied according to the variety.
format	article
author	Erma Prihastanti ENDAH D. HASTUTI SRI W.A. SUEDY
author_facet	Erma Prihastanti ENDAH D. HASTUTI SRI W.A. SUEDY
author_sort	Erma Prihastanti
title	Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods
title_short	Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods
title_full	Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods
title_fullStr	Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods
title_full_unstemmed	Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods
title_sort	short communication: comparing the growth of stem explants between citrus reticulata var. tawangmangu and c. reticulata var. garut using in vitro culture methods
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/6e373ee0800944e1908ed4734b7ff312
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Short Communication: Comparing the growth of stem explants between Citrus reticulata var. Tawangmangu and C. reticulata var. Garut using in vitro culture methods

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