Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.

Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3(+) T cell and F4/8...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu Han Liu, Vijesh Vaghjiani, Jing Yang Tee, Kelly To, Peng Cui, Ding Yuan Oh, Ursula Manuelpillai, Ban-Hock Toh, James Chan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e3a963e955e4e66ab94f88a15e7d859
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6e3a963e955e4e66ab94f88a15e7d859
record_format	dspace
spelling	oai:doaj.org-article:6e3a963e955e4e66ab94f88a15e7d8592021-11-18T07:20:48ZAmniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.1932-620310.1371/journal.pone.0035758https://doaj.org/article/6e3a963e955e4e66ab94f88a15e7d8592012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22563398/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3(+) T cell and F4/80(+) monocyte/macrophage infiltration and demyelination were significantly reduced with hAEC treatment. Besides the known secretion of prostaglandin E2 (PGE2), we report the novel finding that hAEC utilize transforming growth factor-β (TGF-β) for immunosuppression. Neutralization of TGF-β or PGE2 in splenocyte proliferation assays significantly reduced hAEC-induced suppression. Splenocytes from hAEC-treated mice showed a Th2 cytokine shift with significantly elevated IL-5 production. While transferred CFSE-labeled hAEC could be detected in the lung, none were identified in the CNS or in lymphoid organs. This is the first report documenting the therapeutic effect of hAEC in a MS-like model and suggest that hAEC may have potential for use as therapy for MS.Yu Han LiuVijesh VaghjianiJing Yang TeeKelly ToPeng CuiDing Yuan OhUrsula ManuelpillaiBan-Hock TohJames ChanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35758 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu Han Liu Vijesh Vaghjiani Jing Yang Tee Kelly To Peng Cui Ding Yuan Oh Ursula Manuelpillai Ban-Hock Toh James Chan Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.
description	Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3(+) T cell and F4/80(+) monocyte/macrophage infiltration and demyelination were significantly reduced with hAEC treatment. Besides the known secretion of prostaglandin E2 (PGE2), we report the novel finding that hAEC utilize transforming growth factor-β (TGF-β) for immunosuppression. Neutralization of TGF-β or PGE2 in splenocyte proliferation assays significantly reduced hAEC-induced suppression. Splenocytes from hAEC-treated mice showed a Th2 cytokine shift with significantly elevated IL-5 production. While transferred CFSE-labeled hAEC could be detected in the lung, none were identified in the CNS or in lymphoid organs. This is the first report documenting the therapeutic effect of hAEC in a MS-like model and suggest that hAEC may have potential for use as therapy for MS.
format	article
author	Yu Han Liu Vijesh Vaghjiani Jing Yang Tee Kelly To Peng Cui Ding Yuan Oh Ursula Manuelpillai Ban-Hock Toh James Chan
author_facet	Yu Han Liu Vijesh Vaghjiani Jing Yang Tee Kelly To Peng Cui Ding Yuan Oh Ursula Manuelpillai Ban-Hock Toh James Chan
author_sort	Yu Han Liu
title	Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.
title_short	Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.
title_full	Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.
title_fullStr	Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.
title_full_unstemmed	Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.
title_sort	amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6e3a963e955e4e66ab94f88a15e7d859
work_keys_str_mv	AT yuhanliu amnioticepithelialcellsfromthehumanplacentapotentlysuppressamousemodelofmultiplesclerosis AT vijeshvaghjiani amnioticepithelialcellsfromthehumanplacentapotentlysuppressamousemodelofmultiplesclerosis AT jingyangtee amnioticepithelialcellsfromthehumanplacentapotentlysuppressamousemodelofmultiplesclerosis AT kellyto amnioticepithelialcellsfromthehumanplacentapotentlysuppressamousemodelofmultiplesclerosis AT pengcui amnioticepithelialcellsfromthehumanplacentapotentlysuppressamousemodelofmultiplesclerosis AT dingyuanoh amnioticepithelialcellsfromthehumanplacentapotentlysuppressamousemodelofmultiplesclerosis AT ursulamanuelpillai amnioticepithelialcellsfromthehumanplacentapotentlysuppressamousemodelofmultiplesclerosis AT banhocktoh amnioticepithelialcellsfromthehumanplacentapotentlysuppressamousemodelofmultiplesclerosis AT jameschan amnioticepithelialcellsfromthehumanplacentapotentlysuppressamousemodelofmultiplesclerosis
_version_	1718423602984910848

Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.

Ejemplares similares