UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract

As a by-product in the sericulture industry, mulberry branches are not currently utilized effectively. Jialing 20 is an artificial triploids mulberry that widely cultivated in southwest China. In this study, the chemical composition of the Jialing 20 mulberry branch extract (MBE) was first analyzed...

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Autores principales:	Wei Xiang, Zhining Xia, Li Xu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mulberry branch UPLC-MS antioxidant α-glucosidase cholinesterase cardiovascular Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/6e40f01a61084fc2bd85a7d9a67350c6
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spelling	oai:doaj.org-article:6e40f01a61084fc2bd85a7d9a67350c62021-11-25T17:33:58ZUPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract10.3390/foods101126592304-8158https://doaj.org/article/6e40f01a61084fc2bd85a7d9a67350c62021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2659https://doaj.org/toc/2304-8158As a by-product in the sericulture industry, mulberry branches are not currently utilized effectively. Jialing 20 is an artificial triploids mulberry that widely cultivated in southwest China. In this study, the chemical composition of the Jialing 20 mulberry branch extract (MBE) was first analyzed by UPLC-MS/MS, and 42 components, including alkaloids, flavonoids, and coumarins, were obtained. Then, the antioxidant activities, hypoglycemic effect, Alzheimer’s disease inhibition, and cardiovascular protection of MBE were also evaluated in vitro. The IC<sub>50</sub> values for the scavenging DPPH and ABTS radicals were, respectively, 31.23 ± 0.57 μg/mL and 8.88 ± 0.36 μg/mL (IC<sub>50</sub> values of positive Vc were, respectively, 4.41 ± 0.19 μg/mL and 8.79 ± 0.41 μg/mL). The IC<sub>50</sub> value for inhibiting α-glucosidase was 1.90 ± 0.05 μg/mL (IC<sub>50</sub> value of positive acarbose was 0.03 μg/mL). The IC<sub>50</sub> values for inhibiting acetylcholinesterase and butyrylcholinesterase were, respectively, 179.47 ± 0.38 μg/mL and 101.82 ± 3.37 μg/mL (IC<sub>50</sub> values of positive berberine were, respectively, 1.27 ± 0.03 μg/mL and 57.41 ± 0.21 μg/mL). MBE (10 μg/mL and 40 μg/mL) significantly increased the survival rate of oxidized low-density lipoprotein- (ox-LDL) induced human umbilical vein endothelial cells (HUVECs) and significantly decreased the intracellular reactive oxygen species. These results suggest that the extracts of Jialing 20 mulberry branches could be used as a functional food additive.Wei XiangZhining XiaLi XuMDPI AGarticlemulberry branchUPLC-MSantioxidantα-glucosidasecholinesterasecardiovascularChemical technologyTP1-1185ENFoods, Vol 10, Iss 2659, p 2659 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mulberry branch UPLC-MS antioxidant α-glucosidase cholinesterase cardiovascular Chemical technology TP1-1185
spellingShingle	mulberry branch UPLC-MS antioxidant α-glucosidase cholinesterase cardiovascular Chemical technology TP1-1185 Wei Xiang Zhining Xia Li Xu UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract
description	As a by-product in the sericulture industry, mulberry branches are not currently utilized effectively. Jialing 20 is an artificial triploids mulberry that widely cultivated in southwest China. In this study, the chemical composition of the Jialing 20 mulberry branch extract (MBE) was first analyzed by UPLC-MS/MS, and 42 components, including alkaloids, flavonoids, and coumarins, were obtained. Then, the antioxidant activities, hypoglycemic effect, Alzheimer’s disease inhibition, and cardiovascular protection of MBE were also evaluated in vitro. The IC<sub>50</sub> values for the scavenging DPPH and ABTS radicals were, respectively, 31.23 ± 0.57 μg/mL and 8.88 ± 0.36 μg/mL (IC<sub>50</sub> values of positive Vc were, respectively, 4.41 ± 0.19 μg/mL and 8.79 ± 0.41 μg/mL). The IC<sub>50</sub> value for inhibiting α-glucosidase was 1.90 ± 0.05 μg/mL (IC<sub>50</sub> value of positive acarbose was 0.03 μg/mL). The IC<sub>50</sub> values for inhibiting acetylcholinesterase and butyrylcholinesterase were, respectively, 179.47 ± 0.38 μg/mL and 101.82 ± 3.37 μg/mL (IC<sub>50</sub> values of positive berberine were, respectively, 1.27 ± 0.03 μg/mL and 57.41 ± 0.21 μg/mL). MBE (10 μg/mL and 40 μg/mL) significantly increased the survival rate of oxidized low-density lipoprotein- (ox-LDL) induced human umbilical vein endothelial cells (HUVECs) and significantly decreased the intracellular reactive oxygen species. These results suggest that the extracts of Jialing 20 mulberry branches could be used as a functional food additive.
format	article
author	Wei Xiang Zhining Xia Li Xu
author_facet	Wei Xiang Zhining Xia Li Xu
author_sort	Wei Xiang
title	UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract
title_short	UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract
title_full	UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract
title_fullStr	UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract
title_full_unstemmed	UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract
title_sort	uplc-ms/ms profiling, antioxidant, α-glucosidase inhibitory, cholinesterase inhibitory, and cardiovascular protection potentials of jialing 20 (<i>morus multicaulis</i> perr.) mulberry branch extract
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6e40f01a61084fc2bd85a7d9a67350c6
work_keys_str_mv	AT weixiang uplcmsmsprofilingantioxidantaglucosidaseinhibitorycholinesteraseinhibitoryandcardiovascularprotectionpotentialsofjialing20imorusmulticaulisiperrmulberrybranchextract AT zhiningxia uplcmsmsprofilingantioxidantaglucosidaseinhibitorycholinesteraseinhibitoryandcardiovascularprotectionpotentialsofjialing20imorusmulticaulisiperrmulberrybranchextract AT lixu uplcmsmsprofilingantioxidantaglucosidaseinhibitorycholinesteraseinhibitoryandcardiovascularprotectionpotentialsofjialing20imorusmulticaulisiperrmulberrybranchextract
_version_	1718412227737812992

UPLC-MS/MS Profiling, Antioxidant, α-Glucosidase Inhibitory, Cholinesterase Inhibitory, and Cardiovascular Protection Potentials of Jialing 20 (<i>Morus multicaulis</i> Perr.) Mulberry Branch Extract

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