Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression

Abstract Using Chlamydia trachomatis (Ct) as a complex model organism, we describe a method to generate bacterial whole-proteome microarrays using cell-free, on-chip protein expression. Expression constructs were generated by two successive PCRs directly from bacterial genomic DNA. Bacterial protein...

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Autores principales: Katrin Hufnagel, Smiths Lueong, Martina Willhauck-Fleckenstein, Agnes Hotz-Wagenblatt, Beiping Miao, Andrea Bauer, Angelika Michel, Julia Butt, Michael Pawlita, Jörg D. Hoheisel, Tim Waterboer
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/6e5136bb68724a66a4edce0dbcc7a62e
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