Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression
Abstract Using Chlamydia trachomatis (Ct) as a complex model organism, we describe a method to generate bacterial whole-proteome microarrays using cell-free, on-chip protein expression. Expression constructs were generated by two successive PCRs directly from bacterial genomic DNA. Bacterial protein...
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Autores principales: | Katrin Hufnagel, Smiths Lueong, Martina Willhauck-Fleckenstein, Agnes Hotz-Wagenblatt, Beiping Miao, Andrea Bauer, Angelika Michel, Julia Butt, Michael Pawlita, Jörg D. Hoheisel, Tim Waterboer |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2018
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Materias: | |
Acceso en línea: | https://doaj.org/article/6e5136bb68724a66a4edce0dbcc7a62e |
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