Rapid detection of the H275Y oseltamivir resistance mutation in influenza A/H1N1 2009 by single base pair RT-PCR and high-resolution melting.

Rapid detection of the H275Y oseltamivir resistance mutation in influenza A/H1N1 2009 by single base pair RT-PCR and high-resolution melting.

<h4>Introduction</h4>We aimed to design a real-time reverse-transcriptase-PCR (rRT-PCR), high-resolution melting (HRM) assay to detect the H275Y mutation that confers oseltamivir resistance in influenza A/H1N1 2009 viruses.<h4>Findings</h4>A novel strategy of amplifying a sin...

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Autores principales: Steven Y C Tong, Farshid Dakh, Aeron C Hurt, Yi-Mo Deng, Kevin Freeman, Peter K Fagan, Ian G Barr, Philip M Giffard
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2011
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/6e5b1f5b85a949688f884df01347d8af
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