Association of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease
Ebtissam Saleh Al-Meghaiseeb,1 Abdulrahman A Al-Robayan,1 Mulfi Mubarak Al-Otaibi,1 Misbahul Arfin,2 Abdulrahman K Al-Asmari2 1Department of Gastroenterology, 2Research Centre, Prince Sultan Military Medical City, Riyadh, Saudi Arabia Abstract: Inflammatory bowel disease (IBD) is a complex, multifac...
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Dove Medical Press
2016
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oai:doaj.org-article:6e5ea4bf19e849a280903702fc183d622021-12-02T08:52:07ZAssociation of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease1178-7031https://doaj.org/article/6e5ea4bf19e849a280903702fc183d622016-06-01T00:00:00Zhttps://www.dovepress.com/association-of-tumor-necrosis-factor-alpha-and--beta-gene-polymorphism-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Ebtissam Saleh Al-Meghaiseeb,1 Abdulrahman A Al-Robayan,1 Mulfi Mubarak Al-Otaibi,1 Misbahul Arfin,2 Abdulrahman K Al-Asmari2 1Department of Gastroenterology, 2Research Centre, Prince Sultan Military Medical City, Riyadh, Saudi Arabia Abstract: Inflammatory bowel disease (IBD) is a complex, multifactorial, chronic inflammatory disorder of the gastrointestinal tract in which immune dysregulation caused by genetic and/or environmental factors plays an important role. The aim of this case–­control study was to evaluate the association of tumor necrosis factor-alpha (TNF-α) (308) and -β (+252) polymorphisms with susceptibility of IBD. A total of 379 Saudi subjects including 179 IBD patients (ulcerative colitis (UC) =84 and Crohn’s disease (CD) =95) and 200 age- and sex-matched healthy controls were recruited. TNF-a and TNF-b genes were amplified using an amplification refractory mutation systems polymerase chain reaction methodology to detect TNF-α (–308) and -β (+252) polymorphisms. The frequency of the GA genotype of TNF-α (–308G/A) was higher, and the frequencies of the GG and AA genotypes were significantly lower in IBD patients compared with those in controls, indicating that genotype GA-positive individuals are susceptible to IBD and that the GG and AA genotypes exert a protective effect. The frequency of allele A of TNF-α (–308G/A) was significantly higher and that of allele G was lower in IBD patients compared with those in controls, indicating an association of allele A with IBD risk in Saudi patients. On stratification of IBD patients into UC and CD, an almost similar pattern was noticed in both the groups. The results of TNF-β (+252A/G) polymorphisms showed a significant increase in the frequency of the GG genotype in IBD patients, suggesting a positive association of GG genotype with IBD risk. On stratification of IBD patients into UC and CD, the genotype GG of TNF-β was associated with susceptibility risk to UC but not CD. The frequencies of alleles and genotypes of both TNF-α and-β polymorphisms are not affected by sex or type of IBD (familial or sporadic). TNF-α (–308G/A) and TNF-β (+252A/G) polymorphisms are associated with risk of developing IBD in Saudi population. Keywords: tumor necrosis factor, polymorphism, inflammatory bowel disease, Saudis, Crohn’s disease, ulcerative colitis Al-Meghaiseeb ESAl-Robayan AAAl-Otaibi MMArfin MAl-Asmari AKDove Medical PressarticleTumor necrosis factorPolymorphismInflammatory bowel diseaseSaudisCrohn's diseaseulcerative colitisPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2016, Iss Issue 1, Pp 133-140 (2016) |
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DOAJ |
| collection |
DOAJ |
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EN |
| topic |
Tumor necrosis factor Polymorphism Inflammatory bowel disease Saudis Crohn's disease ulcerative colitis Pathology RB1-214 Therapeutics. Pharmacology RM1-950 |
| spellingShingle |
Tumor necrosis factor Polymorphism Inflammatory bowel disease Saudis Crohn's disease ulcerative colitis Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Al-Meghaiseeb ES Al-Robayan AA Al-Otaibi MM Arfin M Al-Asmari AK Association of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease |
| description |
Ebtissam Saleh Al-Meghaiseeb,1 Abdulrahman A Al-Robayan,1 Mulfi Mubarak Al-Otaibi,1 Misbahul Arfin,2 Abdulrahman K Al-Asmari2 1Department of Gastroenterology, 2Research Centre, Prince Sultan Military Medical City, Riyadh, Saudi Arabia Abstract: Inflammatory bowel disease (IBD) is a complex, multifactorial, chronic inflammatory disorder of the gastrointestinal tract in which immune dysregulation caused by genetic and/or environmental factors plays an important role. The aim of this case–­control study was to evaluate the association of tumor necrosis factor-alpha (TNF-α) (308) and -β (+252) polymorphisms with susceptibility of IBD. A total of 379 Saudi subjects including 179 IBD patients (ulcerative colitis (UC) =84 and Crohn’s disease (CD) =95) and 200 age- and sex-matched healthy controls were recruited. TNF-a and TNF-b genes were amplified using an amplification refractory mutation systems polymerase chain reaction methodology to detect TNF-α (–308) and -β (+252) polymorphisms. The frequency of the GA genotype of TNF-α (–308G/A) was higher, and the frequencies of the GG and AA genotypes were significantly lower in IBD patients compared with those in controls, indicating that genotype GA-positive individuals are susceptible to IBD and that the GG and AA genotypes exert a protective effect. The frequency of allele A of TNF-α (–308G/A) was significantly higher and that of allele G was lower in IBD patients compared with those in controls, indicating an association of allele A with IBD risk in Saudi patients. On stratification of IBD patients into UC and CD, an almost similar pattern was noticed in both the groups. The results of TNF-β (+252A/G) polymorphisms showed a significant increase in the frequency of the GG genotype in IBD patients, suggesting a positive association of GG genotype with IBD risk. On stratification of IBD patients into UC and CD, the genotype GG of TNF-β was associated with susceptibility risk to UC but not CD. The frequencies of alleles and genotypes of both TNF-α and-β polymorphisms are not affected by sex or type of IBD (familial or sporadic). TNF-α (–308G/A) and TNF-β (+252A/G) polymorphisms are associated with risk of developing IBD in Saudi population. Keywords: tumor necrosis factor, polymorphism, inflammatory bowel disease, Saudis, Crohn’s disease, ulcerative colitis |
| format |
article |
| author |
Al-Meghaiseeb ES Al-Robayan AA Al-Otaibi MM Arfin M Al-Asmari AK |
| author_facet |
Al-Meghaiseeb ES Al-Robayan AA Al-Otaibi MM Arfin M Al-Asmari AK |
| author_sort |
Al-Meghaiseeb ES |
| title |
Association of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease |
| title_short |
Association of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease |
| title_full |
Association of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease |
| title_fullStr |
Association of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease |
| title_full_unstemmed |
Association of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease |
| title_sort |
association of tumor necrosis factor-α and -β gene polymorphisms in inflammatory bowel disease |
| publisher |
Dove Medical Press |
| publishDate |
2016 |
| url |
https://doaj.org/article/6e5ea4bf19e849a280903702fc183d62 |
| work_keys_str_mv |
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| _version_ |
1718398367989497856 |