Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase

Due to their transient nature, enzyme-substrate complexes are difficult to characterize structurally. Here, the authors capture the reactive reduced form of xenobiotic reductase A bound to its substrate and show that the oxidation state of the flavin cofactor affects the interaction of the substrate...

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Auteurs principaux:	Tobias Werther, Stefan Wahlefeld, Johannes Salewski, Uwe Kuhlmann, Ingo Zebger, Peter Hildebrandt, Holger Dobbek
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2017
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/6e5f0d2118e441349f25a2bde1c8e4d9
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spelling	oai:doaj.org-article:6e5f0d2118e441349f25a2bde1c8e4d92021-12-02T14:41:07ZRedox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase10.1038/ncomms160842041-1723https://doaj.org/article/6e5f0d2118e441349f25a2bde1c8e4d92017-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms16084https://doaj.org/toc/2041-1723Due to their transient nature, enzyme-substrate complexes are difficult to characterize structurally. Here, the authors capture the reactive reduced form of xenobiotic reductase A bound to its substrate and show that the oxidation state of the flavin cofactor affects the interaction of the substrate with the enzyme.Tobias WertherStefan WahlefeldJohannes SalewskiUwe KuhlmannIngo ZebgerPeter HildebrandtHolger DobbekNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Tobias Werther Stefan Wahlefeld Johannes Salewski Uwe Kuhlmann Ingo Zebger Peter Hildebrandt Holger Dobbek Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase
description	Due to their transient nature, enzyme-substrate complexes are difficult to characterize structurally. Here, the authors capture the reactive reduced form of xenobiotic reductase A bound to its substrate and show that the oxidation state of the flavin cofactor affects the interaction of the substrate with the enzyme.
format	article
author	Tobias Werther Stefan Wahlefeld Johannes Salewski Uwe Kuhlmann Ingo Zebger Peter Hildebrandt Holger Dobbek
author_facet	Tobias Werther Stefan Wahlefeld Johannes Salewski Uwe Kuhlmann Ingo Zebger Peter Hildebrandt Holger Dobbek
author_sort	Tobias Werther
title	Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase
title_short	Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase
title_full	Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase
title_fullStr	Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase
title_full_unstemmed	Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase
title_sort	redox-dependent substrate-cofactor interactions in the michaelis-complex of a flavin-dependent oxidoreductase
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6e5f0d2118e441349f25a2bde1c8e4d9
work_keys_str_mv	AT tobiaswerther redoxdependentsubstratecofactorinteractionsinthemichaeliscomplexofaflavindependentoxidoreductase AT stefanwahlefeld redoxdependentsubstratecofactorinteractionsinthemichaeliscomplexofaflavindependentoxidoreductase AT johannessalewski redoxdependentsubstratecofactorinteractionsinthemichaeliscomplexofaflavindependentoxidoreductase AT uwekuhlmann redoxdependentsubstratecofactorinteractionsinthemichaeliscomplexofaflavindependentoxidoreductase AT ingozebger redoxdependentsubstratecofactorinteractionsinthemichaeliscomplexofaflavindependentoxidoreductase AT peterhildebrandt redoxdependentsubstratecofactorinteractionsinthemichaeliscomplexofaflavindependentoxidoreductase AT holgerdobbek redoxdependentsubstratecofactorinteractionsinthemichaeliscomplexofaflavindependentoxidoreductase
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Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase

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