Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma

Background: The chemokine receptor CCR7 is crucial for an intact immune function, but its expression is also associated with clinical outcome in several malignancies. No data exist on the expression of CCR7 in adrenocortical tumors. Methods: CCR7 expression was investigated by qRT-PCR and immunohist...

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Autores principales:	Carmina Teresa Fuss, Katharina Other, Britta Heinze, Laura-Sophie Landwehr, Armin Wiegering, Charis Kalogirou, Stefanie Hahner, Martin Fassnacht
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	CCR7 chemokine receptor adrenocortical carcinoma adrenal tumors Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/6e5f1b1434b84e37aee4d630bada9b3d
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spelling	oai:doaj.org-article:6e5f1b1434b84e37aee4d630bada9b3d2021-11-25T17:02:44ZExpression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma10.3390/cancers132256932072-6694https://doaj.org/article/6e5f1b1434b84e37aee4d630bada9b3d2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5693https://doaj.org/toc/2072-6694Background: The chemokine receptor CCR7 is crucial for an intact immune function, but its expression is also associated with clinical outcome in several malignancies. No data exist on the expression of CCR7 in adrenocortical tumors. Methods: CCR7 expression was investigated by qRT-PCR and immunohistochemistry in 4 normal adrenal glands, 59 adrenocortical adenomas, and 181 adrenocortical carcinoma (ACC) samples. Results: CCR7 is highly expressed in the outer adrenocortical zones and medulla. Aldosterone-producing adenomas showed lower CCR7 protein levels (H-score 1.3 ± 1.0) compared to non-functioning (2.4 ± 0.5) and cortisol-producing adenomas (2.3 ± 0.6), whereas protein expression was variable in ACC (1.8 ± 0.8). In ACC, CCR7 protein expression was significantly higher in lymph node metastases (2.5 ± 0.5) compared to primary tumors (1.8±0.8) or distant metastases (2.0 ± 0.4; <i>p</i> < 0.01). mRNA levels of CCR7 were not significantly different between ACCs, normal adrenals, and adrenocortical adenomas. In contrast to other tumor entities, neither CCR7 protein nor mRNA expression significantly impacted patients’ survival. Conclusion: We show that CCR7 is expressed on mRNA and protein level across normal adrenals, benign adrenocortical tumors, as well as ACCs. Given that CCR7 did not influence survival in ACC, it is probably not involved in tumor progression, but it could play a role in adrenocortical homeostasis.Carmina Teresa FussKatharina OtherBritta HeinzeLaura-Sophie LandwehrArmin WiegeringCharis KalogirouStefanie HahnerMartin FassnachtMDPI AGarticleCCR7chemokine receptoradrenocortical carcinomaadrenal tumorsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5693, p 5693 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CCR7 chemokine receptor adrenocortical carcinoma adrenal tumors Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	CCR7 chemokine receptor adrenocortical carcinoma adrenal tumors Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Carmina Teresa Fuss Katharina Other Britta Heinze Laura-Sophie Landwehr Armin Wiegering Charis Kalogirou Stefanie Hahner Martin Fassnacht Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma
description	Background: The chemokine receptor CCR7 is crucial for an intact immune function, but its expression is also associated with clinical outcome in several malignancies. No data exist on the expression of CCR7 in adrenocortical tumors. Methods: CCR7 expression was investigated by qRT-PCR and immunohistochemistry in 4 normal adrenal glands, 59 adrenocortical adenomas, and 181 adrenocortical carcinoma (ACC) samples. Results: CCR7 is highly expressed in the outer adrenocortical zones and medulla. Aldosterone-producing adenomas showed lower CCR7 protein levels (H-score 1.3 ± 1.0) compared to non-functioning (2.4 ± 0.5) and cortisol-producing adenomas (2.3 ± 0.6), whereas protein expression was variable in ACC (1.8 ± 0.8). In ACC, CCR7 protein expression was significantly higher in lymph node metastases (2.5 ± 0.5) compared to primary tumors (1.8±0.8) or distant metastases (2.0 ± 0.4; <i>p</i> < 0.01). mRNA levels of CCR7 were not significantly different between ACCs, normal adrenals, and adrenocortical adenomas. In contrast to other tumor entities, neither CCR7 protein nor mRNA expression significantly impacted patients’ survival. Conclusion: We show that CCR7 is expressed on mRNA and protein level across normal adrenals, benign adrenocortical tumors, as well as ACCs. Given that CCR7 did not influence survival in ACC, it is probably not involved in tumor progression, but it could play a role in adrenocortical homeostasis.
format	article
author	Carmina Teresa Fuss Katharina Other Britta Heinze Laura-Sophie Landwehr Armin Wiegering Charis Kalogirou Stefanie Hahner Martin Fassnacht
author_facet	Carmina Teresa Fuss Katharina Other Britta Heinze Laura-Sophie Landwehr Armin Wiegering Charis Kalogirou Stefanie Hahner Martin Fassnacht
author_sort	Carmina Teresa Fuss
title	Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma
title_short	Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma
title_full	Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma
title_fullStr	Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma
title_full_unstemmed	Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma
title_sort	expression of the chemokine receptor ccr7 in the normal adrenal gland and adrenal tumors and its correlation with clinical outcome in adrenocortical carcinoma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6e5f1b1434b84e37aee4d630bada9b3d
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Expression of the Chemokine Receptor CCR7 in the Normal Adrenal Gland and Adrenal Tumors and Its Correlation with Clinical Outcome in Adrenocortical Carcinoma

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