The leukocyte telomere length, single nucleotide polymorphisms near TERC gene and risk of COPD

Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction and is associated with chronic local and systemic inflammation and oxidative stress. The enhanced oxidative stress and inflammation have been reported to affect telomere length (TL). Furthermore, a numb...

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Autores principales:	Tanya Tacheva, Shanbeh Zienolddiny, Dimo Dimov, Denitsa Vlaykova, Tatyana Vlaykova
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	COPD Telomeres Telomerase Polymorphism Medicine R
Acceso en línea:	https://doaj.org/article/6e60c1e57cee4ecd89ca3c88590d8288
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spelling	oai:doaj.org-article:6e60c1e57cee4ecd89ca3c88590d82882021-11-13T15:05:05ZThe leukocyte telomere length, single nucleotide polymorphisms near TERC gene and risk of COPD10.7717/peerj.121902167-8359https://doaj.org/article/6e60c1e57cee4ecd89ca3c88590d82882021-11-01T00:00:00Zhttps://peerj.com/articles/12190.pdfhttps://peerj.com/articles/12190/https://doaj.org/toc/2167-8359Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction and is associated with chronic local and systemic inflammation and oxidative stress. The enhanced oxidative stress and inflammation have been reported to affect telomere length (TL). Furthermore, a number of SNPs at loci encoding the main components of the telomerase genes, TERT and TERC have been shown to correlate with TL. We aimed to explore the leukocyte TL and genotypes for single nucleotide polymorphisms, rs12696304 (C > G) and rs10936599 (C > T) near TERC in COPD cases and matched healthy controls using q-PCR technologies. Successful assessment of TL was performed for 91 patients and 88 controls. The patients had shorter TL (17919.36 ± 1203.01 bp) compared to controls (21 271.48 ± 1891.36 bp) although not significant (p = 0.137). The TL did not associate with the gender, age, spirometric indexes, smoking habits but tended to correlate negatively with BMI (Rho = − 0.215, p = 0.076) in the controls, but not in COPD patients. The genotype frequencies of the SNPs rs12696304 and rs10936599 were compared between patients and controls and the odds ratios (OR) for developing COPD were calculated. The carriers of the common homozygous (CC) genotypes of the SNPs had higher risk for COPD, compared to carriers of the variants alleles (rs12696304 CG+GG vs. CC; OR: 0.615, 95% CI [0.424–0.894], p = 0.011 and for rs10936599 CT+TT vs. CC OR = 0.668, 95% CI [0.457–0.976], p = 0.044). Analysis on the combined effects of the TERC rs12696304 (C > G) and rs10936599 (C > T) genotypes, CC/CC genotype combination was associated with higher risk for COPD (p < 0.0001) and marginally lower FEV1% pr. in patients with GOLD II (p = 0.052). There was no association between the SNP genotypes and TL. In summary, our results suggest that COPD patients may have shorter TL, and rs12696304 and rs10936599 near TERC may affect the risk of COPD independently of TL.Tanya TachevaShanbeh ZienolddinyDimo DimovDenitsa VlaykovaTatyana VlaykovaPeerJ Inc.articleCOPDTelomeresTelomerasePolymorphismMedicineRENPeerJ, Vol 9, p e12190 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COPD Telomeres Telomerase Polymorphism Medicine R
spellingShingle	COPD Telomeres Telomerase Polymorphism Medicine R Tanya Tacheva Shanbeh Zienolddiny Dimo Dimov Denitsa Vlaykova Tatyana Vlaykova The leukocyte telomere length, single nucleotide polymorphisms near TERC gene and risk of COPD
description	Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction and is associated with chronic local and systemic inflammation and oxidative stress. The enhanced oxidative stress and inflammation have been reported to affect telomere length (TL). Furthermore, a number of SNPs at loci encoding the main components of the telomerase genes, TERT and TERC have been shown to correlate with TL. We aimed to explore the leukocyte TL and genotypes for single nucleotide polymorphisms, rs12696304 (C > G) and rs10936599 (C > T) near TERC in COPD cases and matched healthy controls using q-PCR technologies. Successful assessment of TL was performed for 91 patients and 88 controls. The patients had shorter TL (17919.36 ± 1203.01 bp) compared to controls (21 271.48 ± 1891.36 bp) although not significant (p = 0.137). The TL did not associate with the gender, age, spirometric indexes, smoking habits but tended to correlate negatively with BMI (Rho = − 0.215, p = 0.076) in the controls, but not in COPD patients. The genotype frequencies of the SNPs rs12696304 and rs10936599 were compared between patients and controls and the odds ratios (OR) for developing COPD were calculated. The carriers of the common homozygous (CC) genotypes of the SNPs had higher risk for COPD, compared to carriers of the variants alleles (rs12696304 CG+GG vs. CC; OR: 0.615, 95% CI [0.424–0.894], p = 0.011 and for rs10936599 CT+TT vs. CC OR = 0.668, 95% CI [0.457–0.976], p = 0.044). Analysis on the combined effects of the TERC rs12696304 (C > G) and rs10936599 (C > T) genotypes, CC/CC genotype combination was associated with higher risk for COPD (p < 0.0001) and marginally lower FEV1% pr. in patients with GOLD II (p = 0.052). There was no association between the SNP genotypes and TL. In summary, our results suggest that COPD patients may have shorter TL, and rs12696304 and rs10936599 near TERC may affect the risk of COPD independently of TL.
format	article
author	Tanya Tacheva Shanbeh Zienolddiny Dimo Dimov Denitsa Vlaykova Tatyana Vlaykova
author_facet	Tanya Tacheva Shanbeh Zienolddiny Dimo Dimov Denitsa Vlaykova Tatyana Vlaykova
author_sort	Tanya Tacheva
title	The leukocyte telomere length, single nucleotide polymorphisms near TERC gene and risk of COPD
title_short	The leukocyte telomere length, single nucleotide polymorphisms near TERC gene and risk of COPD
title_full	The leukocyte telomere length, single nucleotide polymorphisms near TERC gene and risk of COPD
title_fullStr	The leukocyte telomere length, single nucleotide polymorphisms near TERC gene and risk of COPD
title_full_unstemmed	The leukocyte telomere length, single nucleotide polymorphisms near TERC gene and risk of COPD
title_sort	leukocyte telomere length, single nucleotide polymorphisms near terc gene and risk of copd
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/6e60c1e57cee4ecd89ca3c88590d8288
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The leukocyte telomere length, single nucleotide polymorphisms near TERC gene and risk of COPD

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