Apobec 3G efficiently reduces infectivity of the human exogenous gammaretrovirus XMRV.

<h4>Background</h4>The human exogenous gammaretrovirus XMRV is thought to be implicated in prostate cancer and chronic fatigue syndrome. Besides pressing epidemiologic questions, the elucidation of the tissue and cell tropism of the virus, as well as its sensitivity to retroviral restric...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kristin Stieler, Nicole Fischer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e64feb7013946b7bd4244efffdbec1d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6e64feb7013946b7bd4244efffdbec1d
record_format	dspace
spelling	oai:doaj.org-article:6e64feb7013946b7bd4244efffdbec1d2021-12-02T20:19:48ZApobec 3G efficiently reduces infectivity of the human exogenous gammaretrovirus XMRV.1932-620310.1371/journal.pone.0011738https://doaj.org/article/6e64feb7013946b7bd4244efffdbec1d2010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20668529/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The human exogenous gammaretrovirus XMRV is thought to be implicated in prostate cancer and chronic fatigue syndrome. Besides pressing epidemiologic questions, the elucidation of the tissue and cell tropism of the virus, as well as its sensitivity to retroviral restriction factors is of fundamental importance. The Apobec3 (A3) proteins, a family of cytidine deaminases, are one important group of host proteins that control primary infection and efficient viral spread.<h4>Methodology/principal findings</h4>Here we demonstrate that XMRV is resistant to human Apobec 3B, 3C and 3F, while being highly susceptible to the human A3G protein, a factor which is known to confer antiviral activity against most retroviruses. We show that XMRV as well as MoMLV virions package Apobec proteins independent of their specific restriction activity. hA3G was found to be a potent inhibitor of XMRV as well as of MoMLV infectivity. In contrast to MoMLV, XMRV infection can also be partially reduced by low concentrations of mA3. Interestingly, established prostate cancer cell lines, which are highly susceptible to XMRV infection, do not or only weakly express hA3G.<h4>Conclusions</h4>Our findings confirm and extend recently published data that show restriction of XMRV infection by hA3G. The results will be of value to explore which cells are infected with XMRV and efficiently support viral spread in vivo. Furthermore, the observation that XMRV infection can be reduced by mA3 is of interest with regard to the current natural reservoir of XMRV infection.Kristin StielerNicole FischerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11738 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristin Stieler Nicole Fischer Apobec 3G efficiently reduces infectivity of the human exogenous gammaretrovirus XMRV.
description	<h4>Background</h4>The human exogenous gammaretrovirus XMRV is thought to be implicated in prostate cancer and chronic fatigue syndrome. Besides pressing epidemiologic questions, the elucidation of the tissue and cell tropism of the virus, as well as its sensitivity to retroviral restriction factors is of fundamental importance. The Apobec3 (A3) proteins, a family of cytidine deaminases, are one important group of host proteins that control primary infection and efficient viral spread.<h4>Methodology/principal findings</h4>Here we demonstrate that XMRV is resistant to human Apobec 3B, 3C and 3F, while being highly susceptible to the human A3G protein, a factor which is known to confer antiviral activity against most retroviruses. We show that XMRV as well as MoMLV virions package Apobec proteins independent of their specific restriction activity. hA3G was found to be a potent inhibitor of XMRV as well as of MoMLV infectivity. In contrast to MoMLV, XMRV infection can also be partially reduced by low concentrations of mA3. Interestingly, established prostate cancer cell lines, which are highly susceptible to XMRV infection, do not or only weakly express hA3G.<h4>Conclusions</h4>Our findings confirm and extend recently published data that show restriction of XMRV infection by hA3G. The results will be of value to explore which cells are infected with XMRV and efficiently support viral spread in vivo. Furthermore, the observation that XMRV infection can be reduced by mA3 is of interest with regard to the current natural reservoir of XMRV infection.
format	article
author	Kristin Stieler Nicole Fischer
author_facet	Kristin Stieler Nicole Fischer
author_sort	Kristin Stieler
title	Apobec 3G efficiently reduces infectivity of the human exogenous gammaretrovirus XMRV.
title_short	Apobec 3G efficiently reduces infectivity of the human exogenous gammaretrovirus XMRV.
title_full	Apobec 3G efficiently reduces infectivity of the human exogenous gammaretrovirus XMRV.
title_fullStr	Apobec 3G efficiently reduces infectivity of the human exogenous gammaretrovirus XMRV.
title_full_unstemmed	Apobec 3G efficiently reduces infectivity of the human exogenous gammaretrovirus XMRV.
title_sort	apobec 3g efficiently reduces infectivity of the human exogenous gammaretrovirus xmrv.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/6e64feb7013946b7bd4244efffdbec1d
work_keys_str_mv	AT kristinstieler apobec3gefficientlyreducesinfectivityofthehumanexogenousgammaretrovirusxmrv AT nicolefischer apobec3gefficientlyreducesinfectivityofthehumanexogenousgammaretrovirusxmrv
_version_	1718374168955715584

Apobec 3G efficiently reduces infectivity of the human exogenous gammaretrovirus XMRV.

Ejemplares similares