The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.

The ability to reproduce is essential in all branches of life. In metazoans, this process is initiated by formation of the germline, a group of cells that are destined to form the future gonads, the tissue that will produce the gametes. The molecular mechanisms underlying germline formation differs...

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Autores principales:	Yin Ho Vong, Lavanya Sivashanmugam, Rebecca Leech, Andreas Zaucker, Alex Jones, Karuna Sampath
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/6e6948addf58422b8ec77c196822fdba
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spelling	oai:doaj.org-article:6e6948addf58422b8ec77c196822fdba2021-12-02T20:02:58ZThe RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.1553-73901553-740410.1371/journal.pgen.1009667https://doaj.org/article/6e6948addf58422b8ec77c196822fdba2021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009667https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The ability to reproduce is essential in all branches of life. In metazoans, this process is initiated by formation of the germline, a group of cells that are destined to form the future gonads, the tissue that will produce the gametes. The molecular mechanisms underlying germline formation differs between species. In zebrafish, development of the germline is dependent on the specification, migration and proliferation of progenitors called the primordial germ cells (PGCs). PGC specification is dependent on a maternally provided cytoplasmic complex of ribonucleoproteins (RNPs), the germplasm. Here, we show that the conserved RNA-binding protein (RBP), Igf2bp3, has an essential role during early embryonic development and germline development. Loss of Igf2bp3 leads to an expanded yolk syncytial layer (YSL) in early embryos, reduced germline RNA expression, and mis-regulated germline development. We show that loss of maternal Igf2bp3 function results in translational de-regulation of a Nodal reporter during the mid-blastula transition. Furthermore, maternal igf2bp3 mutants exhibit reduced expression of germplasm transcripts, defects in chemokine guidance, abnormal PGC behavior and germ cell death. Consistently, adult igf2bp3 mutants show a strong male bias. Our findings suggest that Igf2bp3 is essential for normal embryonic and germline development, and acts as a key regulator of sexual development.Yin Ho VongLavanya SivashanmugamRebecca LeechAndreas ZauckerAlex JonesKaruna SampathPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 7, p e1009667 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Yin Ho Vong Lavanya Sivashanmugam Rebecca Leech Andreas Zaucker Alex Jones Karuna Sampath The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.
description	The ability to reproduce is essential in all branches of life. In metazoans, this process is initiated by formation of the germline, a group of cells that are destined to form the future gonads, the tissue that will produce the gametes. The molecular mechanisms underlying germline formation differs between species. In zebrafish, development of the germline is dependent on the specification, migration and proliferation of progenitors called the primordial germ cells (PGCs). PGC specification is dependent on a maternally provided cytoplasmic complex of ribonucleoproteins (RNPs), the germplasm. Here, we show that the conserved RNA-binding protein (RBP), Igf2bp3, has an essential role during early embryonic development and germline development. Loss of Igf2bp3 leads to an expanded yolk syncytial layer (YSL) in early embryos, reduced germline RNA expression, and mis-regulated germline development. We show that loss of maternal Igf2bp3 function results in translational de-regulation of a Nodal reporter during the mid-blastula transition. Furthermore, maternal igf2bp3 mutants exhibit reduced expression of germplasm transcripts, defects in chemokine guidance, abnormal PGC behavior and germ cell death. Consistently, adult igf2bp3 mutants show a strong male bias. Our findings suggest that Igf2bp3 is essential for normal embryonic and germline development, and acts as a key regulator of sexual development.
format	article
author	Yin Ho Vong Lavanya Sivashanmugam Rebecca Leech Andreas Zaucker Alex Jones Karuna Sampath
author_facet	Yin Ho Vong Lavanya Sivashanmugam Rebecca Leech Andreas Zaucker Alex Jones Karuna Sampath
author_sort	Yin Ho Vong
title	The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.
title_short	The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.
title_full	The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.
title_fullStr	The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.
title_full_unstemmed	The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.
title_sort	rna-binding protein igf2bp3 is critical for embryonic and germline development in zebrafish.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6e6948addf58422b8ec77c196822fdba
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The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.

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