Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease

Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds causing important economic losses in the poultry industry worldwide. We have previously developed a plant-based vaccine candidate for infectious bursal disea...

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Autores principales:	María Soledad Lucero, Silvina Chimeno Zoth, Juan Jaton, María José Gravisaco, Silvina Pinto, Matías Richetta, Analía Berinstein, Evangelina Gómez
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	plant-based vaccine infectious bursal disease VP2 oral immunization chickens Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/6e6acd818c144b32bd6f0321e415a754
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spelling	oai:doaj.org-article:6e6acd818c144b32bd6f0321e415a7542021-11-18T05:37:45ZOral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease1664-462X10.3389/fpls.2021.741469https://doaj.org/article/6e6acd818c144b32bd6f0321e415a7542021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.741469/fullhttps://doaj.org/toc/1664-462XInfectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds causing important economic losses in the poultry industry worldwide. We have previously developed a plant-based vaccine candidate for infectious bursal disease (IBD) that is able to protect against infection with IBDV when administered through intramuscular (im) route. Given that oral vaccination is non-invasive and stimulates the immunity of the mucosal gastrointestinal surface, the initial site of contact and entry of IBDV, the aim of this work was to study if our immunogen was also able to elicit a protective immune response when orally administered. We demonstrated that 85% of the animals that received two oral doses of the vaccine formulation and all animals that were orally boosted after an im prime scheme developed virus neutralizing antibodies and were protected against IBDV infection, evidenced by the bursa/body weight (BB) ratio, absence of T-cell infiltration, and low viral load in bursa. Although mild to moderate bursal damage was observed in some of these animals, these lesions were not as severe as the ones observed in challenged control groups, which also presented signs of acute inflammation, bursal atrophy, T-cell infiltration, and absence of viral clearance. These results show that two immunizations with our recombinant immunogen are able to induce a specific and protective immune response in chicken against IBDV when orally administered in a prime/boost scheme or when the oral boost follows an im prime scheme. In conclusion, our oral plant-based vaccine candidate could represent a viable alternative to conventional vaccines and is of great interest to the poultry industry.María Soledad LuceroSilvina Chimeno ZothJuan JatonMaría José GravisacoSilvina PintoMatías RichettaAnalía BerinsteinEvangelina GómezFrontiers Media S.A.articleplant-based vaccineinfectious bursal diseaseVP2oral immunizationchickensPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant-based vaccine infectious bursal disease VP2 oral immunization chickens Plant culture SB1-1110
spellingShingle	plant-based vaccine infectious bursal disease VP2 oral immunization chickens Plant culture SB1-1110 María Soledad Lucero Silvina Chimeno Zoth Juan Jaton María José Gravisaco Silvina Pinto Matías Richetta Analía Berinstein Evangelina Gómez Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease
description	Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds causing important economic losses in the poultry industry worldwide. We have previously developed a plant-based vaccine candidate for infectious bursal disease (IBD) that is able to protect against infection with IBDV when administered through intramuscular (im) route. Given that oral vaccination is non-invasive and stimulates the immunity of the mucosal gastrointestinal surface, the initial site of contact and entry of IBDV, the aim of this work was to study if our immunogen was also able to elicit a protective immune response when orally administered. We demonstrated that 85% of the animals that received two oral doses of the vaccine formulation and all animals that were orally boosted after an im prime scheme developed virus neutralizing antibodies and were protected against IBDV infection, evidenced by the bursa/body weight (BB) ratio, absence of T-cell infiltration, and low viral load in bursa. Although mild to moderate bursal damage was observed in some of these animals, these lesions were not as severe as the ones observed in challenged control groups, which also presented signs of acute inflammation, bursal atrophy, T-cell infiltration, and absence of viral clearance. These results show that two immunizations with our recombinant immunogen are able to induce a specific and protective immune response in chicken against IBDV when orally administered in a prime/boost scheme or when the oral boost follows an im prime scheme. In conclusion, our oral plant-based vaccine candidate could represent a viable alternative to conventional vaccines and is of great interest to the poultry industry.
format	article
author	María Soledad Lucero Silvina Chimeno Zoth Juan Jaton María José Gravisaco Silvina Pinto Matías Richetta Analía Berinstein Evangelina Gómez
author_facet	María Soledad Lucero Silvina Chimeno Zoth Juan Jaton María José Gravisaco Silvina Pinto Matías Richetta Analía Berinstein Evangelina Gómez
author_sort	María Soledad Lucero
title	Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease
title_short	Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease
title_full	Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease
title_fullStr	Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease
title_full_unstemmed	Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease
title_sort	oral immunization with plant-based vaccine induces a protective response against infectious bursal disease
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6e6acd818c144b32bd6f0321e415a754
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Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease

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