Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals

This study evaluated the presence of Human Papillomavirus (HPV) DNA in the cervix and peripheral blood of women with cervical intraepithelial neoplasia (CIN I, II, and III) and healthy individuals. Overall, 139 paired peripheral blood and cervix samples of healthy women and women with CIN I, II, and...

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Autores principales:	Kamylla Conceição Gomes Nascimento, Élyda Gonçalves Lima, Zhilbelly Mota Nunes, Marconi Rêgo Barros Júnior, Marcus Vinícius de Aragão Batista, Antonio Roberto Lucena Araujo, Jacinto da Costa Silva Neto, Bárbara Simas Chagas, Ana Pavla Almeida Diniz Gurgel, Antonio Carlos de Freitas
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	human papillomavirus DNA cervix peripheral blood cervical lesions healthy individuals Medicine R
Acceso en línea:	https://doaj.org/article/6e737adee35542aeb57fde7609a8599a
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spelling	oai:doaj.org-article:6e737adee35542aeb57fde7609a8599a2021-11-11T17:48:54ZDetection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals10.3390/jcm102152092077-0383https://doaj.org/article/6e737adee35542aeb57fde7609a8599a2021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5209https://doaj.org/toc/2077-0383This study evaluated the presence of Human Papillomavirus (HPV) DNA in the cervix and peripheral blood of women with cervical intraepithelial neoplasia (CIN I, II, and III) and healthy individuals. Overall, 139 paired peripheral blood and cervix samples of healthy women and women with CIN I, II, and III (<i>n</i> = 68) were tested for HPV DNA by using standard procedures. Polymerase chain reaction (PCR) sequencing determined HPV types. Quantification of HPV16 E6 and E2 genes was performed to determine viral load and physical state. HPV DNA was detected in the cervix (21.1% in healthy individuals; 48.8–55.5% in CIN patients), blood (46.4% in healthy individuals; 44.1–77.7% in CIN patients) and paired peripheral blood and cervix samples (24% in healthy individuals; 32.5–44.4% in CIN patients). The most frequent types found in the cervix were HPV16, 18, 31, 33, 58, and 70, while HPV16, 18, 33, 58, and 66 were the most frequent types found in the blood. HPV DNA in the cervix was associated with previous sexually transmitted infections (STIs) (<i>p</i> = 0.023; OR: 2.978; CI:1.34–7.821), HPV DNA in the blood (<i>p</i> = 0.000; OR: 8.283; CI:3.700–18.540), and cervical lesions (CIN I/II or III) (<i>p</i> = 0.007). Binomial logistic regression showed that HPV DNA in the blood (<i>p</i> = 0.000; OR: 9.324; CI:3.612–24.072) and cervical lesions (<i>p</i> = 0.011; OR: 3.622; CI:1.338–9.806) were associated with HPV DNA in the cervix. However, we did not find an association between HPV DNA in the blood and cervical lesions (<i>p</i> = 0.385). Our results showed that only HPV DNA found in the cervix was associated with cervical lesions.Kamylla Conceição Gomes NascimentoÉlyda Gonçalves LimaZhilbelly Mota NunesMarconi Rêgo Barros JúniorMarcus Vinícius de Aragão BatistaAntonio Roberto Lucena AraujoJacinto da Costa Silva NetoBárbara Simas ChagasAna Pavla Almeida Diniz GurgelAntonio Carlos de FreitasMDPI AGarticlehuman papillomavirus DNAcervixperipheral bloodcervical lesionshealthy individualsMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5209, p 5209 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	human papillomavirus DNA cervix peripheral blood cervical lesions healthy individuals Medicine R
spellingShingle	human papillomavirus DNA cervix peripheral blood cervical lesions healthy individuals Medicine R Kamylla Conceição Gomes Nascimento Élyda Gonçalves Lima Zhilbelly Mota Nunes Marconi Rêgo Barros Júnior Marcus Vinícius de Aragão Batista Antonio Roberto Lucena Araujo Jacinto da Costa Silva Neto Bárbara Simas Chagas Ana Pavla Almeida Diniz Gurgel Antonio Carlos de Freitas Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals
description	This study evaluated the presence of Human Papillomavirus (HPV) DNA in the cervix and peripheral blood of women with cervical intraepithelial neoplasia (CIN I, II, and III) and healthy individuals. Overall, 139 paired peripheral blood and cervix samples of healthy women and women with CIN I, II, and III (<i>n</i> = 68) were tested for HPV DNA by using standard procedures. Polymerase chain reaction (PCR) sequencing determined HPV types. Quantification of HPV16 E6 and E2 genes was performed to determine viral load and physical state. HPV DNA was detected in the cervix (21.1% in healthy individuals; 48.8–55.5% in CIN patients), blood (46.4% in healthy individuals; 44.1–77.7% in CIN patients) and paired peripheral blood and cervix samples (24% in healthy individuals; 32.5–44.4% in CIN patients). The most frequent types found in the cervix were HPV16, 18, 31, 33, 58, and 70, while HPV16, 18, 33, 58, and 66 were the most frequent types found in the blood. HPV DNA in the cervix was associated with previous sexually transmitted infections (STIs) (<i>p</i> = 0.023; OR: 2.978; CI:1.34–7.821), HPV DNA in the blood (<i>p</i> = 0.000; OR: 8.283; CI:3.700–18.540), and cervical lesions (CIN I/II or III) (<i>p</i> = 0.007). Binomial logistic regression showed that HPV DNA in the blood (<i>p</i> = 0.000; OR: 9.324; CI:3.612–24.072) and cervical lesions (<i>p</i> = 0.011; OR: 3.622; CI:1.338–9.806) were associated with HPV DNA in the cervix. However, we did not find an association between HPV DNA in the blood and cervical lesions (<i>p</i> = 0.385). Our results showed that only HPV DNA found in the cervix was associated with cervical lesions.
format	article
author	Kamylla Conceição Gomes Nascimento Élyda Gonçalves Lima Zhilbelly Mota Nunes Marconi Rêgo Barros Júnior Marcus Vinícius de Aragão Batista Antonio Roberto Lucena Araujo Jacinto da Costa Silva Neto Bárbara Simas Chagas Ana Pavla Almeida Diniz Gurgel Antonio Carlos de Freitas
author_facet	Kamylla Conceição Gomes Nascimento Élyda Gonçalves Lima Zhilbelly Mota Nunes Marconi Rêgo Barros Júnior Marcus Vinícius de Aragão Batista Antonio Roberto Lucena Araujo Jacinto da Costa Silva Neto Bárbara Simas Chagas Ana Pavla Almeida Diniz Gurgel Antonio Carlos de Freitas
author_sort	Kamylla Conceição Gomes Nascimento
title	Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals
title_short	Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals
title_full	Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals
title_fullStr	Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals
title_full_unstemmed	Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals
title_sort	detection of human papillomavirus dna in paired peripheral blood and cervix samples in patients with cervical lesions and healthy individuals
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6e737adee35542aeb57fde7609a8599a
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Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals

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